Advance in the induction of the differentiation of pluripotent stem cells into corneal epithelial cells

doi:10.3877/cma.j.issn.2095-1221.2017.06.008

Abstract

Abstract:

The induction of the differentiation of pluripotent stem cells into corneal epithelial cells for the treatment of limbal stem cell deficiency hasbroad prospect. Currently, great achievements have been made in the technique of inducing the pluripotent stem cells into the corneal epithelial cells. However, problems such as low efficiency and long induction time still exist. In order to provide seed cells with high proliferative ability for the reconstruction of ocular surface and provide reference and guidance for clinical application, we summarized the methods of including pluripotent stem cells into the corneal epithelial cells. Additionally, we analyzed the clinical application prospect of pluripotent stem cells differentiate into corneal epithelial cells.

Key words: Pluripotent stem cells, Corneal epithelial cells, Limbal stem cell deficiency

Rong Xiong, Gang Feng, Zhanmei Li. Advance in the induction of the differentiation of pluripotent stem cells into corneal epithelial cells[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2017, 07(06): 356-359.

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URL: https://zhxbygxbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-1221.2017.06.008

https://zhxbygxbzz.cma-cmc.com.cn/EN/Y2017/V07/I06/356

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References 24

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来源	诱导方法	诱导培养基	特殊添加物	饲养层细胞	血清	载体	阳性率	诱导时间
小鼠IPS^[14]	模拟微环境	DMEM/F12（3/1）	bFGF、EGF、NGF	角膜缘成纤维细胞	10﹪ FCS	Ⅳ型胶原	13﹪	12 d
人ESCs^[15]	模拟微环境	DMEM/F12（3/1）和LFCM	EGF、腺嘌呤、霍乱毒素、胰岛素	3T3成纤维细胞	10﹪ FCS	Ⅳ型胶原、粘连蛋白、纤连蛋白	\	21 d
人IPS^[16]	模拟微环境	GMEM	NEAA、2-ME、丙酮酸钠	MMC-treated PA6 feeder layer	10﹪KSR	无	\	12~16 weeks
人ESCs^[17]	模拟微环境	mTeSR-1和LFCM	无	无	无	基质胶	99﹪	16 ~ 21 d
人IPS^[21]	小分子诱导	RegES medium	SB-505124；IWP-2 bFGF	无	无	Ⅳ型胶原	95﹪	5 weeks

来源	诱导方法	诱导培养基	特殊添加物	饲养层细胞	血清	载体	阳性率	诱导时间
小鼠IPS^[14]	模拟微环境	DMEM/F12（3/1）	bFGF、EGF、NGF	角膜缘成纤维细胞	10﹪ FCS	Ⅳ型胶原	13﹪	12 d
人ESCs^[15]	模拟微环境	DMEM/F12（3/1）和LFCM	EGF、腺嘌呤、霍乱毒素、胰岛素	3T3成纤维细胞	10﹪ FCS	Ⅳ型胶原、粘连蛋白、纤连蛋白	\	21 d
人IPS^[16]	模拟微环境	GMEM	NEAA、2-ME、丙酮酸钠	MMC-treated PA6 feeder layer	10﹪KSR	无	\	12~16 weeks
人ESCs^[17]	模拟微环境	mTeSR-1和LFCM	无	无	无	基质胶	99﹪	16 ~ 21 d
人IPS^[21]	小分子诱导	RegES medium	SB-505124；IWP-2 bFGF	无	无	Ⅳ型胶原	95﹪	5 weeks

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