Advances in 3D bioprinting in the field of kidney regeneration

doi:10.3877/cma.j.issn.2095-1221.2024.03.009

Abstract

Abstract:

The incidence of chronic kidney disease (CKD) continues to increase globally and progresses to end-stage renal disease (ESRD) . For patients with ESRD, dialysis or kidney transplantation has always been the leading clinical treatment. However, dialysis treatment is associated with several complications and limits a patient's lifestyle and social activities. Kidney transplantation is another viable treatment option for ESRD but is limited due to issues such as donor shortage and rejection. Therefore, many strategies for kidney regeneration are under investigation. As a tissue engineering technology, 3D bioprinting has made progress in fabricating in vitro kidney tissue models and kidney-like structures. It is expected to solve the problem of organ donor shortage by creating artificial organs/tissues. While the technology has been adapted to a variety of cell types and biomaterials, many issues still need to be addressed. Examples include cell and biomaterial selection and simulating vascular networks. This article aims to review the latest progress of 3D bioprinting in the field of kidney regeneration, including commonly used 3D bioprinting technologies and biomaterial selection, 3D bioprinting to generate partial nephrons, and challenges and future development directions.

Key words: 3D bioprinting, Bioink, Kidney cells, Induced pluripotent stem cells

Xuyan Guo, Zhirong Luo, Qi Xue, Linmeng Wang, Yunhua Ji, Bo Zhang. Advances in 3D bioprinting in the field of kidney regeneration[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2024, 14(03): 181-185.

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References 49

1	Layton AT. Modeling transport and flow regulatory mechanisms of the kidney[J]. ISRN Biomath, 2012, 2012(2012):170594. doi:10.5402/2012/170594.
2	Jansen K, Schuurmans CCL, Jansen J, et al. Hydrogel-based cell therapies for kidney regeneration: current trends in biofabrication and in vivo repair[J]. Curr Pharm Des, 2017, 23(26):3845-3857.
3	Jansen J, Fedecostante M, Wilmer MJ, et al. Biotechnological challenges of bioartificial kidney engineering[J]. Biotechnol Adv, 2014, 32(7):1317-1327.
4	Mota C, Camarero-Espinosa S, Baker MB, et al. Bioprinting: from tissue and organ development to in vitro models[J]. Chem Rev, 2020, 120(19):10547-10607.
5	Wu Y, Qin M, Yang X. Organ bioprinting: progress, challenges and outlook[J]. J Mater Chem B, 2023, 11(43):10263-10287.
6	Mittal R, Woo FW, Castro CS, et al. Organ-on-chip models: implications in drug discovery and clinical applications[J]. J Cell Physiol, 2019, 234(6):8352-80.
7	Kačarević ŽP, Rider PM, Alkildani S, et al. An introduction to 3d bioprinting: possibilities, challengesand future aspects[J]. Materials (Basel), 2018, 11(11):2199. doi:10.3390/ma11112199.
8	Xu K, Han Y, Huang Y, et al. The application of 3d bioprinting in urological diseases[J]. Mater Today Bio, 2022, 16:100388. doi: 10.1016/j.mtbio.2022.100388.
9	Hu S, Yi Y, Ye C, et al. Advances in 3d printing techniques for cartilage regeneration of temporomandibular joint disc and mandibular condyle[J]. Int J Bioprint, 2023, 9(5):761. doi: 10.18063/ijb.761.
10	Lawlor KT, Vanslambrouck JM, Higgins JW, et al. Cellular extrusion bioprinting improves kidney organoid reproducibility and conformation[J]. Nat Mater, 2021, 20(2):260-271.
11	Lin NYC, Homan KA, Robinson SS, et al. Renal reabsorption in 3d vascularized proximal tubule models[J]. Proc Natl Acad Sci U S A, 2019, 116(12):5399-5404.
12	Datta P, Barui A, Wu Y, et al. Essential steps in bioprinting: from pre-to post-bioprinting[J]. Biotechnol Adv, 2018, 36(5):1481-1504.
13	Graham AD, Olof SN, Burke MJ, et al. High-resolution patterned cellular constructs by droplet-based 3d printing[J]. Sci Rep, 2017, 7(1):7004. doi: 10.1038/s41598-017-06358-x.
14	Liang R, Gu Y, Wu Y, et al. Lithography-based 3d bioprinting and bioinks for bone repair and regeneration[J]. ACS Biomater Sci Eng, 2021, 7(3):806-816.
15	Gao J, Liu X, Cheng J, et al. Application of photocrosslinkable hydrogels based on photolithography 3d bioprinting technology in bone tissue engineering[J]. Regen Biomater, 2023, 10:rbad037. doi: 10.1093/rb/rbad037.
16	Xu F, Ren H, Zheng M, et al. Development of biodegradable bioactive glass ceramics by dlp printed containing epcs/bmscs for bone tissue engineering of rabbit mandible defects[J]. J Mech Behav Biomed Mater, 2020, 103:103532. doi: 10.1016/j.jmbbm.2019.103532.
17	Ma X, Yu C, Wang P, et al. Rapid 3d bioprinting of decellularized extracellular matrix with regionally varied mechanical properties and biomimetic microarchitecture[J]. Biomaterials, 2018, 185:310-321.
18	Grigoryan B, Paulsen SJ, Corbett DC, et al. Multivascular networks and functional intravascular topologies within biocompatible hydrogels[J]. Science, 2019, 364(6439):458-464.
19	Rosette KA, Lander SM, VanOpstall C, et al. Three-dimensional coculture provides an improved in vitro model for papillary renal cell carcinoma[J]. Am J Physiol Renal Physiol, 2021, 321(1):F33-F46.
20	Mota C, Camarero-Espinosa S, Baker MB, et al. Bioprinting: from tissue and organ development to in vitro models[J]. Chemical Reviews, 2020, 120(19):10547-10607.
21	Yoon J, Han H, Jang J. Nanomaterials-incorporated hydrogels for 3d bioprinting technology[J]. Nano Converg, 2023, 10(1):52. doi: 10.1186/s40580-023-00402-5.
22	Gadre M, Kasturi M, Agarwal P, et al. Decellularization and their significance for tissue regeneration in the era of 3d bioprinting[J]. ACS Omega, 2024, 9(7):7375-7392.
23	Ali M, Pr AK, Yoo JJ, et al. A photo-crosslinkable kidney ecm-derived bioink accelerates renal tissue formation[J]. Adv Healthc Mater, 2019, 8(7):e1800992. doi: 10.1002/adhm.201800992.
24	Singh NK, Han W, Nam SA, et al. Three-dimensional cell-printing of advanced renal tubular tissue analogue[J]. Biomaterials, 2020, 232:119734. doi: 10.1016/j.biomaterials.2019.119734.
25	Homaeigohar S, Tsai TY, Young TH, et al. An electroactive alginate hydrogel nanocomposite reinforced by functionalized graphite nanofilaments for neural tissue engineering[J]. Carbohydr Polym, 2019, 224:115112. doi: 10.1016/j.carbpol.2019.115112.
26	Gauvin R, Chen YC, Lee JW, et al. Microfabrication of complex porous tissue engineering scaffolds using 3d projection stereolithography[J]. Biomaterials, 2012, 33(15):3824-3834.
27	Pi Q, Maharjan S, Yan X, et al. Digitally tunable microfluidic bioprinting of multilayered cannular tissues[J]. Adv Mater, 2018, 30(43):e1706913. doi: 10.1002/adma.201706913.
28	Kim J H, Lee S, Kang SJ, et al. Establishment of three-dimensional bioprinted bladder cancer-on-a-chip with a microfluidic system using bacillus calmette-guérin[J]. Int J Mol Sci, 2021, 22(16):8887. doi: 10.3390/ijms22168887.
29	Kim MJ, Chi BH, Yoo JJ, et al. Structure establishment of three-dimensional (3d) cell culture printing model for bladder cancer[J]. PLoS One, 2019, 14(10):e0223689. doi: 10.1371/journal.pone.0223689.
30	Butler HM, Naseri E, MacDonald DS, et al. Investigation of rheology, printability, and biocompatibility of n,o-carboxymethyl chitosan and agarose bioinks for 3d bioprinting of neuron cells[J]. Materialia, 2021, 18:101169. doi: 10.1016/j.mtla.2021.101169.
31	Zarrintaj P, Manouchehri S, Ahmadi Z, et al. Agarose-based biomaterials for tissue engineering[J]. Carbohydrate Polymers, 2018, 187:66-84.
32	Tetsuka H, Shin SR. Materials and technical innovations in 3d printing in biomedical applications[J]. J Mater Chem B, 2020, 8(15):2930-2950.
33	Choudhury D, Anand S, Naing MW. The arrival of commercial bioprinters-towards 3d bioprinting revolution![J]. Int J Bioprint, 2018, 4(2):139. doi: 10.18063/IJB.v4i2.139.
34	Gao B, Yang Q, Zhao X, et al. 4D bioprinting for biomedical applications[J]. Trends in Biotechnology, 2016, 34(9):746-756.
35	Wang X, Jiang M, Zhou Z, et al. 3D printing of polymer matrix composites: a review and prospective[J]. Composites Part B: Engineering, 2017, 110:442-458.
36	Mironov V, Drake C, Wen X. Research project: charleston bioengineered kidney project[J]. Biotechnol J, 2006, 1(9):903-905.
37	Kolesky DB, Homan KA, Skylar-Scott MA, et al. Three-dimensional bioprinting of thick vascularized tissues[J]. Proc Natl Acad Sci U S A, 2016, 113(12):3179-3184.
38	Homan KA, Kolesky DB, Skylar-Scott MA, et al. Bioprinting of 3d convoluted renal proximal tubules on perfusable chips[J]. Sci Rep, 2016, 6:34845. doi: 10.1038/srep34845.
39	Jung JW, Lee JS, Cho DW. Computer-aided multiple-head 3d printing system for printing of heterogeneous organ/tissue constructs[J]. Sci Rep, 2016, 6:21685. doi: 10.1038/srep21685.
40	Sämfors S, Karlsson K, Sundberg J, et al. Biofabrication of bacterial nanocellulose scaffolds with complex vascular structure[J]. Biofabrication, 2019, 11(4):045010. doi: 10.1088/1758-5090/ab2b4f.
41	King SM, Higgins JW, Nino CR, et al. 3D proximal tubule tissues recapitulate key aspects of renal physiology to enable nephrotoxicity testing[J]. Front Physiol, 2017, 8:123. doi: 10.3389/fphys.2017.00123.
42	Kolesky DB, Homan KA, Skylar-Scott M, et al. In vitro human tissues via multi-material 3-D bioprinting[J]. Altern Lab Anim, 2018, 46(4):209-215.
43	Addario G, Djudjaj S, Farè S, et al. Microfluidic bioprinting towards a renal in vitro model[J]. Bioprinting, 2020, 20:e00108. doi: 10.3389/fphys.2022.1048738.
44	Czerniecki SM, Cruz NM, Harder JL, et al. High-throughput screening enhances kidney organoid differentiation from human pluripotent stem cells and enables automated multidimensional phenotyping[J]. Cell Stem Cell, 2018, 22(6):929-40.e4.
45	Huang G, Zhao Y, Chen D, et al. Applications, advancements, and challenges of 3d bioprinting in organ transplantation[J]. Biomater Sci, 2024, 12(6):1425-1448.
46	Yu Y, Li X, Li Y, et al. Derivation and characterization of endothelial cells from porcine induced pluripotent stem cells[J]. Int J Mol Sci, 2022, 23(13):7029. doi: 10.3390/ijms23137029.
47	Alizadeh S, Mahboobi L, Nasiri M, et al. Decellularized placental sponge seeded with human mesenchymal stem cells improves deep skin wound healing in the animal model[J]. ACS Appl Bio Mater, 2024, 7(4):2140-2152.
48	Zhu W, Ma X, Gou M, et al. 3D printing of functional biomaterials for tissue engineering[J]. Curr Opin Biotechnol, 2016, 40:103-112.
49	Balakhovsky YM, Ostrovskiy AY, Khesuani YD. Emerging business models toward commercialization of bioprinting technology[C]. Cham: Springer International Publishing, 2017: 1-22.

作者	模型	细胞	生物材料	生物打印技术
Kim等^[24]	近端小管	人脐静脉内皮细胞、肾近端小管上皮细胞、人肾皮质近曲小管上皮细胞	脱细胞外基质、海藻酸钠、普朗尼克	挤出式打印
King等^[41]	近端小管	肾成纤维细胞、人脐静脉内皮细胞、肾近端小管上皮细胞	热敏生物材料油墨	挤出式打印
Homan等^[11,42]	有或没有血管系统的近端小管模型	人脐静脉内皮细胞、肾近端小管上皮细胞、肾小球微血管内皮细胞	明胶、纤维蛋白、普朗尼克	挤出式打印
Ali等^[23]	近端小管、肾小球	人原代细胞	脱细胞外基质、明胶、透明质酸、甘油	挤出式打印
Addario等^[43]	肾小管间质	原代小鼠上皮细胞、人脐静脉内皮细胞	藻酸盐、果胶和明胶	挤出式打印（微流体核-壳）
Lawlor等^[10]	肾类器官模型	诱导多能干细胞衍生的肾类器官	无油墨生物材料	挤出生物打印（基于活塞）
Harder等^[44]	肾类器官模型	胚胎或诱导多能干细胞衍生的肾类器官	无油墨生物材料	液体处理分配系统

作者	模型	细胞	生物材料	生物打印技术
Kim等^[24]	近端小管	人脐静脉内皮细胞、肾近端小管上皮细胞、人肾皮质近曲小管上皮细胞	脱细胞外基质、海藻酸钠、普朗尼克	挤出式打印
King等^[41]	近端小管	肾成纤维细胞、人脐静脉内皮细胞、肾近端小管上皮细胞	热敏生物材料油墨	挤出式打印
Homan等^[11,42]	有或没有血管系统的近端小管模型	人脐静脉内皮细胞、肾近端小管上皮细胞、肾小球微血管内皮细胞	明胶、纤维蛋白、普朗尼克	挤出式打印
Ali等^[23]	近端小管、肾小球	人原代细胞	脱细胞外基质、明胶、透明质酸、甘油	挤出式打印
Addario等^[43]	肾小管间质	原代小鼠上皮细胞、人脐静脉内皮细胞	藻酸盐、果胶和明胶	挤出式打印（微流体核-壳）
Lawlor等^[10]	肾类器官模型	诱导多能干细胞衍生的肾类器官	无油墨生物材料	挤出生物打印（基于活塞）
Harder等^[44]	肾类器官模型	胚胎或诱导多能干细胞衍生的肾类器官	无油墨生物材料	液体处理分配系统

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