To investigate the effects and the mechanism of induced pluripotent stem cells (iPSCs) induced hematopoietic stem cells on endothelial cell angiogenesis in vitro experiments.

iPS cells were induced to differentiate into hematopoietic stem cells (iPS-HSCs) in vitro. The experiment was divided into two groups: human umbilical vein endothelial cells (HUVECs) group and iPS-HSC+HUVEC group. The effects of iPS-HSCs on the angiogenesis of endothelial cells were observed in the Matrigel, and the relative expression levels of vascular endothelial growth factor (VEGF) and angiopoietin 1 (Ang-1) protein were detected by Western blot.

The co-culture of iPS-HSCs and HUVECs could significantly promote the tube formation of endothelial cells, and the protein expression levels of VEGF and Ang-1 were significantly increased. Compared with HUVEC group, the number of branch points in iPS-HSC+HUVEC group (10.33±1.53 vs 28.67±3.51) was significantly increased (t = 8.292, P < 0.01) ; the relative expression level of VEGF in iPS-HSC+HUVEC group (0.86±0.09 vs 1.44±0.11) was significantly increased (t = 43.715, P < 0.01) ; the relative expression level of Ang-1 in iPS-HSC+HUVEC group (0.46±0.01 vs 0.68±0.05) was significantly higher (t =52.255, P < 0.01) .

The hematopoietic stem cells induced by iPS cells can promote the angiogenesis of endothelial cells, which may be related to the up-regulation of VEGF and Ang-1 proteins.