Application of pluripotent stem cells in establishing heart model in vitro

doi:10.3877/cma.j.issn.2095-1221.2022.05.009

Abstract

Abstract:

Traditional two-dimensional cell models and model organisms are not fully applicable to the basic research of cardiovascular diseases due to their own limitations. Pluripotent stem cells (PSCs) , including embryonic stem cells (ESCs) and induced PSCs (iPSCs) , are characterized by the properties of self-renewal and potency. In recent years, more and more studies have constructed in vitro cardiac models with PSCs. At present, PSCs can be induced to differentiate into a variety of cardiac lineage cells, tissue engineered hearts can be obtained by combining tissue engineering techniques, and spontaneous organoid models can be obtained in 3D culture systems. The in vitro heart model obtained by PSCs can partially repaint the natural development process of human heart, and it is more similar to the biological characteristics and functions of the heart in vivo than the traditional model, showing good application prospects.

Key words: In vitro model, Pluripotent stem cells, Heart tissues, Organoids

Yuan Gao, Wei Sheng, Guoying Huang. Application of pluripotent stem cells in establishing heart model in vitro[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2022, 12(05): 314-318.

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