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中华细胞与干细胞杂志(电子版) ›› 2020, Vol. 10 ›› Issue (05) : 314 -318. doi: 10.3877/cma.j.issn.2095-1221.2020.05.011

所属专题： 文献；

综述

三维培养的间充质干细胞球体的生物学特性及应用的研究进展
唐敏英1, 雷艳2, 詹世淮3, 李荣春3, 路君1, 吴仲秋4,†()   
  1. 1. 350025　福州，联勤保障部队第900医院基础医学实验室；350025　福州，联勤保障部队第900医院福建省移植生物学重点实验室
    2. 350025　福州，联勤保障部队第900医院检验科
    3. 350025　福州，联勤保障部队第900医院基础医学实验室
    4. 350025　福州，联勤保障部队第900医院超声诊断科
  • 收稿日期:2020-06-01 出版日期:2020-10-01
  • 通信作者: 吴仲秋
  • 基金资助:
    联勤保障部队第900医院院内课题战时伤病救治研究专项(2018Z05)

Advances in biological characteristics and applications of mesenchymal stem cells spheroids under three-dimensional culture

Minying Tang1, Yan Lei2, Shihuai Zhan3, Rongchun Li3, Jun Lu1, Zhongqiu Wu4,†()   

  1. 1. Basic Medical Laboratory, the 900th Hospital of Joint Logistics Support Force, Fuzhou 350025, China; Key Laboratory of Transplant Biology, the 900th Hospital of Joint Logistics Support Force, Fuzhou 350025, China
    2. Clinical Laboratory, the 900th Hospital of Joint Logistics Support Force, Fuzhou 350025, China
    3. Basic Medical Laboratory, the 900th Hospital of Joint Logistics Support Force, Fuzhou 350025, China
    4. Department of Ultrasound Diagnosis, the 900th Hospital of Joint Logistics Support Force, Fuzhou 350025, China
  • Received:2020-06-01 Published:2020-10-01
  • Corresponding author: Zhongqiu Wu
  • About author:
    Corresponding author:Wu Zhongqiu, Email:
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唐敏英, 雷艳, 詹世淮, 李荣春, 路君, 吴仲秋. 三维培养的间充质干细胞球体的生物学特性及应用的研究进展[J/OL]. 中华细胞与干细胞杂志(电子版), 2020, 10(05): 314-318.

Minying Tang, Yan Lei, Shihuai Zhan, Rongchun Li, Jun Lu, Zhongqiu Wu. Advances in biological characteristics and applications of mesenchymal stem cells spheroids under three-dimensional culture[J/OL]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2020, 10(05): 314-318.

二维（2D）贴壁培养是间充质干细胞体外扩增的标准技术，但其局限性也很明显，比如组织特异性的结构、生物学行为及细胞间的相互作用在2D环境中缺失。三维（3D）培养是一种模拟体内生长环境的细胞培养方式，通过让细胞聚集成3D球体或者将细胞在成分结构类似于实体组织的3D结构载体上黏附、伸展和生长，从时间和空间上共同调控细胞的增殖和分化，使特性和功能得以较大程度保留。与2D培养相比，3D培养增强了细胞与细胞之间以及细胞与胞外基质之间的相互作用，更准确地模拟细胞在体内的自然微环境。3D培养也解决了MSCs在体外增殖能力减低的问题，MSCs成脂、成骨和成软骨的能力增强。MSCs球体的制备（微球体黏附培养）被认为是提高MSCs细胞疗法的优化方式之一，MSCs球体的形成能增强抗炎作用、增强血管生成、增加组织再生和修复作用并提高MSCs移植后的存活率。本文就微球体黏附培养MSCs的生物学特性及其应用进展进行综合阐述。

关键词: 间充质干细胞, 三维培养, 血管生成, 组织修复, 骨组织再生

Traditionally, two-dimensional (2D) adherent culture conditions have been used as a standard technique for in vitro expansion of mesenchymal stem cells (MSCs) , but their limitations are also obvious, such as the absence of tissue-specific structures, biological behavior and cellular interactions in the two-dimensional environment. In contrast, 3D cell culture is regarded as a mode simulating the growth environment in vivo. By aggregating cells into 3D spheres or attaching, extending, and growing on three-dimensional structural vectors whose components are similar to solid tissue, cell proliferation and differentiation are regulated in both time- and space-dependent manners. Thus, the characteristics and functions of cells cultured in 3D can be better preserved. The 3D spheroid culture enhances cell-cell and cell-extracellular matrix (ECM) interactions and closely mimics the natural microenvironment of a tissue as compared with traditional 2D monolayer culture. In addition, 3D culture also solves the problem of decreased proliferation of MSCs in vitro, and the capabilities of adipogenesis, osteogenesis and chondrogenesis of MSC spheroids were significantly enhanced in 3D culture. In conclusion, the preparation of MSCs spheroids (microsphere adhesion culture) is considered as one of the optimal ways to improve MSCs-based cell therapy. The application of 3D MSCs spheroids can enhance anti-inflammatory effect, promote angiogenesis, increase tissue regeneration and repair, and improve the survival rate of MSCs after transplantation. In this review, we discuss the biological characteristics and applications of MSCs spheroids under three-dimensional culture.

Key words: Mesenchymal stem cells, Three-dimensional culture, Angiogenesis, Tissue repair, Bone tissue regeneration
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