Advances in the function and mechanism of hypoxic preconditioned mesenchymal stem cells

doi:10.3877/cma.j.issn.2095-1221.2023.02.008

Abstract

Abstract:

Mesenchymal stem cells (MSCs) therapy is currently a hot spot in the field of regenerative medicine. It has received extensive attention and research due to its various biological activities such as immune regulation, tissue repair, and hematopoiesis. But in clinical applications, MSCs transplantation faces challenges such as low survival rates and limited functionality. In recent years, great progress has been made in improving the biological activity of MSCs by using physical, chemical and biological stimulation pretreatments, and hypoxia is one of the most widely used pretreatment methods. This article critically examines the impact and mechanisms of hypoxic preconditioning on various functions of MSCs such as proliferation, apoptosis, differentiation, homing, paracrine signaling, angiogenesis, and immune response.

Key words: Hypoxic preconditioning, Mesenchymal stem cells, HIF-1α

Yuting Chen, Ying Zhou, Yafei Lu, Bin Jiang. Advances in the function and mechanism of hypoxic preconditioned mesenchymal stem cells[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2023, 13(02): 115-120.

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References 63

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MSCs来源	氧浓度	培养时间（d）	结果	机制
牙龈^[28]	3%	2	神经元分化增加	诱导更多与神经元发育相关的基因表达
骨髓^[29]	1%	1/3	神经胶质细胞分化增强	激活HIF-1α/VEGF通路
脂肪^[30]	5%	14	平滑肌细胞分化增强	激活TGF-β/smad2通路
脐带^[31]	CoCl₂	2	软骨分化增强	上调软骨分化调节基因SOX9表达
脂肪^[32]	0.5%	2	软骨分化增强	抑制p38 MAPK通路
骨髓^[33]	CoCl₂	4	成骨分化增强	激活OPG/ RANKL/RANK通路
骨髓^[34]	CoCl₂	7	成骨分化增强	激活HIF-1α/ VEGF信号通路
骨髓^[35]	2%	14	成骨分化减弱	下调成骨分化基因Runx2表达
牙周韧带^[36]	3%+ CoCl₂	3	成骨分化增强	激活HIF-1α/ VEGF/ Runx2通路
外周血^[37,38]	1%	21	成骨分化减弱	上调了Notch-1表达
	9%		成骨分化增强	下调了Notch-1表达
睾丸^[39]	5%	7	脂肪分化减弱	下调脂肪生成基因C/EBPα、PPARG表达

MSCs来源	氧浓度	培养时间（d）	结果	机制
牙龈^[28]	3%	2	神经元分化增加	诱导更多与神经元发育相关的基因表达
骨髓^[29]	1%	1/3	神经胶质细胞分化增强	激活HIF-1α/VEGF通路
脂肪^[30]	5%	14	平滑肌细胞分化增强	激活TGF-β/smad2通路
脐带^[31]	CoCl₂	2	软骨分化增强	上调软骨分化调节基因SOX9表达
脂肪^[32]	0.5%	2	软骨分化增强	抑制p38 MAPK通路
骨髓^[33]	CoCl₂	4	成骨分化增强	激活OPG/ RANKL/RANK通路
骨髓^[34]	CoCl₂	7	成骨分化增强	激活HIF-1α/ VEGF信号通路
骨髓^[35]	2%	14	成骨分化减弱	下调成骨分化基因Runx2表达
牙周韧带^[36]	3%+ CoCl₂	3	成骨分化增强	激活HIF-1α/ VEGF/ Runx2通路
外周血^[37,38]	1%	21	成骨分化减弱	上调了Notch-1表达
	9%		成骨分化增强	下调了Notch-1表达
睾丸^[39]	5%	7	脂肪分化减弱	下调脂肪生成基因C/EBPα、PPARG表达

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