Research progress in large-scale cultivation of mesenchymal stem cells in vitro

doi:10.3877/cma.j.issn.2095-1221.2023.06.007

Abstract

Abstract:

Mesenchymal stem cells are a type of stem cells with self-renewal and multi- directional differentiation potential, which have functions such as immune regulation and tissue repair. With the wide application of mesenchymal stem cells in clinical practice, there is an increasing demand for cell expansion in vitro to obtain many clinical-grade cells. Therefore, getting a sufficient quantity of mesenchymal stem cells in vitro is the core problem to be solved in mesenchymal stem cell culture, preparation and clinical application. In this paper, we summarize the advantages and limitations of the current two-dimensional and three-dimensional large-scale cultivation methods for mesenchymal stem cells and the practical applications of large-scale cultivation. It also looks forward to the development trends of large-scale, automatic and intelligent cultivation systems.

Key words: Mesenchymal stem cells, Two-dimensional culture, Three-dimensional culture, Large-scale cultivation

Wenyu Zhang, Yuxiang Huang. Research progress in large-scale cultivation of mesenchymal stem cells in vitro[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2023, 13(06): 370-376.

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References 58

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培养方式		优点	局限性
2D大规模培养	多层细胞培养瓶培养	生产工艺变动小；细胞不存在剪切损伤	放大规模有限；成本较高
	细胞工厂培养	培养环境安全；污染风险小；可实现大规模培养	层数较多的细胞工厂需要机械辅助来支持培养基的添加和移除
3D大规模培养	微载体培养	降低生产成本；污染风险小；可实现大规模培养及自动化生产；	容易聚团；细胞存在剪切损伤
	微囊化培养	防止培养过程中的聚团；细胞不存在剪切损伤	制作过程复杂；成功率低；无法实现连续化生产
	细胞微球培养	细胞存活时间长；便于储存和运输；可实现大规模培养	制备过程复杂；制备效率低；细胞存在剪切损伤
	中空纤维系统培养	适用范围广；细胞不存在剪切损伤；可实现大规模培养	无法实时观察细胞状态；细胞收获难；胰酶消化时间长

培养方式		优点	局限性
2D大规模培养	多层细胞培养瓶培养	生产工艺变动小；细胞不存在剪切损伤	放大规模有限；成本较高
	细胞工厂培养	培养环境安全；污染风险小；可实现大规模培养	层数较多的细胞工厂需要机械辅助来支持培养基的添加和移除
3D大规模培养	微载体培养	降低生产成本；污染风险小；可实现大规模培养及自动化生产；	容易聚团；细胞存在剪切损伤
	微囊化培养	防止培养过程中的聚团；细胞不存在剪切损伤	制作过程复杂；成功率低；无法实现连续化生产
	细胞微球培养	细胞存活时间长；便于储存和运输；可实现大规模培养	制备过程复杂；制备效率低；细胞存在剪切损伤
	中空纤维系统培养	适用范围广；细胞不存在剪切损伤；可实现大规模培养	无法实时观察细胞状态；细胞收获难；胰酶消化时间长

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