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中华细胞与干细胞杂志(电子版) ›› 2017, Vol. 07 ›› Issue (06) : 351 -355. doi: 10.3877/cma.j.issn.2095-1221.2017.06.007

所属专题: 文献

综述

细胞膜片技术在组织工程中的应用
郭海林1, 贾智明1, 鲍星奇1, 谢华1, 陈方1,()   
  1. 1. 200062 上海交通大学附属儿童医院 上海市儿童医院泌尿外科
  • 收稿日期:2017-08-31 出版日期:2017-12-01
  • 通信作者: 陈方
  • 基金资助:
    上海交通大学博士创新基金(BXJ201741); 国家自然科学基金(81470911)

Application of the cell sheet technology in tissue engineering

Hailin Guo1, Zhiming Jia1, Xingqi Bao1, Hua Xie1, Fang Chen1,()   

  1. 1. Department of Urology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200062, China
  • Received:2017-08-31 Published:2017-12-01
  • Corresponding author: Fang Chen
  • About author:
    Corresponding author: Chen Fang, Email:
引用本文:

郭海林, 贾智明, 鲍星奇, 谢华, 陈方. 细胞膜片技术在组织工程中的应用[J/OL]. 中华细胞与干细胞杂志(电子版), 2017, 07(06): 351-355.

Hailin Guo, Zhiming Jia, Xingqi Bao, Hua Xie, Fang Chen. Application of the cell sheet technology in tissue engineering[J/OL]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2017, 07(06): 351-355.

细胞膜片技术在组织工程中显示了良好的应用前景。与传统胰酶消化收集细胞的方法相比,运用该技术收集到的细胞保留了体外培养过程中分泌的胞外基质、建立的细胞-基质连接以及细胞间连接等结构。目前细胞膜片技术已被应用到角膜、食管、心脏、牙周韧带及软骨等多种组织修复中。然而,应用细胞膜片技术构建富血管化的大块组织、细胞膜片技术的工业化进程及基于细胞膜片技术的再生医学被大众所认知和接受仍是其在组织工程中面临的挑战。本文就细胞膜片技术在组织工程中的研究现状及最新进展作一综述,并寻求出其在临床及基础研究中的进一步发展方向。

Tissue engineering using whole, intact cell sheets has shown promise in many cell-based therapies. Avoiding the use of enzymes, expended cells can be harvested together with endogenous extracellular matrix, cell-matrix contacts and cell-cell contacts. Currently, the cell sheet engineering has already been applied in various diseases, including the cornea, esophagus, heart, periodontal ligament, and cartilage. However, several challenges stand in the way of the vascularization of the big mass constructs, the industrialization of cell sheet products and the widespread acceptance of regenerative medicine based on cell sheet engineering. In this review, we introduce recent progress in the engineering of cell sheets and propose the directions of cell sheet technology in foundation and clinical studies.

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