Interleukin-4 reduces the supportive ability of umbilical cord mesenchymal stem cells on differentiation potentialof hematopoietic stem cells

doi:10.3877/cma.j.issn.2095-1221.2019.01.006

Abstract

Abstract:

Objective

Umbilical cord mesenchymal stem cells (UC-MSC) are a hot research field because of its unique advantages. UC-MSC possess the ability of maintaince the differentiation potential of hematopoietic stem cells, and we studied the effect of interleukin-4 on this ability.

Methods

Umbilical cord mesenchymal stem cells were co-cultured with cord blood CD34⁺ cells with or without IL-4. CD34⁺ cells were counted after 14 days. Moreover, colony forming ability of cells was evaluated. CD11b, CD14 and CD15 expression of cells from colony forming assay was assayed by flow cytometry. Statistical analysis was performed by unpaired t-test. After IL-4 was added, the number of cells (1.31±0.05) ×10⁵ cells/well decreased significantly compared with the control group (2.80±0.28) ×10⁵ cells/well (t = 7.31, P < 0.05) , and flow cytometry analysis showed that the proportion of CD34⁺ cells in the co-culture system also decreased. The ability of IL-?4-treated cells to form colony forming unit-macrophage (9.33±1.53 per well) was significantly lower than that of the control group (17.67±0.58 per well, t = 8.84, P < 0.001) ; The ability to form colony forming unit-granulocyte/macrophage (15.67±3.22 per well) was significantly lower than that of the control group (29.33±4.04 per well, t = 4.58, P < 0.05) . The ability to form total colony forming units (39.33±9.07 per well) was also lower than that of the control group (62.67±6.66 per well, t = 3.59, P < 0.05) . The total number of cells differentiated from IL-4 group (3.67±1.71) ×10⁵ cells/well was also significantly lower than that of the control group (9.50±3.13) ×10⁵ cells/?well, (t = 2.83, P < 0.05) , and flow cytometry analysis showed that the ratio of CD14⁺ cells in differentiated cells was also lower.

Conclusion

IL-4 can reduce the maintenance ability of umbilical cord mesenchymal stem cells on hematopoietic stem cells differentiation, suggesting that when mesenchymal stem cells are used in treatment of type II helper T cell-related humoral immune diseases, the hematopoietic related functions should also be considered.

Key words: Umbilical cordmesenchymal stem cells, Hematopoietic stem cells, Differentiation potential, Interleukin-4

Dongyang Guo, Yan Lu, Yingxing Yue, Zhouxin Yang. Interleukin-4 reduces the supportive ability of umbilical cord mesenchymal stem cells on differentiation potentialof hematopoietic stem cells[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2019, 09(01): 29-34.

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Figures/Tables 5

References 13

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分组	样本数	BFU-E	CFU-G	CFU-M	CFU-GM	CFU- GEMM	总集落形成单位	总细胞数（×10⁵）
对照组	3	1.00±1.00	13.33±3.22	17.67±0.58	29.33±4.04	1.33±1.53	62.67±6.66	9.50±3.13
IL-4组	3	1.00±1.73	12.33±2.52	9.33±1.53	15.67±3.22	1.00±1.00	39.33±9.07	3.67±1.71
t值	?	0.00	0.42	8.84	4.58	0.32	3.59	2.83
P值	?	0.99	0.69	< 0.01	< 0.05	0.77	< 0.05	< 0.05

分组	样本数	BFU-E	CFU-G	CFU-M	CFU-GM	CFU- GEMM	总集落形成单位	总细胞数（×10⁵）
对照组	3	1.00±1.00	13.33±3.22	17.67±0.58	29.33±4.04	1.33±1.53	62.67±6.66	9.50±3.13
IL-4组	3	1.00±1.73	12.33±2.52	9.33±1.53	15.67±3.22	1.00±1.00	39.33±9.07	3.67±1.71
t值	?	0.00	0.42	8.84	4.58	0.32	3.59	2.83
P值	?	0.99	0.69	< 0.01	< 0.05	0.77	< 0.05	< 0.05

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