Effect of umbilical cord mesenchymal stem cells in combination with UM171 on amplification of cord blood CD34+ cells

doi:10.3877/cma.j.issn.2095-1221.2017.02.006

Abstract

Abstract:

Objective

To investigate the effect of umbilical cord mesenchymal stem cells (MSCs) in combination with UM171 on amplification of cord blood CD34⁺cells.

Methods

The cord blood CD34⁺cells and umbilical cord MSCs were divided into four groups: Group A (control group) , Group B (UM171 culture group) , Group C (MSCs co-culture group) and Group D (UM171 and MSCs co-culture group) . The cell number, phenotype and colony formation ability were compared among different groups after amplification in vitro for 10 days.

Results

The umbilical cord MSCs expressed CD105, CD73 and CD90, while didn't express CD14, CD34, CD19, CD45 and HLA-DR. After induction, the cells could differentiate into osteoblasts, adipocytes and chondrocytes. After the CD34⁺cells were culturedin vitrofor 10 days, the total nuclear cells in the UM171 culture group were amplified by 14 times, and CD34⁺cells by 13.5 times; the total nuclear cells in the MSCs co-culture group were amplified by 11 times, and CD34⁺cells by 10 times; the total nuclear cells in the UM171 and MSCs co-culture group were amplified by 22 times, and CD34⁺cells by 21 times.The ratio of CD34⁺CD38^-cell in the UM171 and MSCs co-culture group was (91.49±2.67) ﹪. There was significant difference compared with the UM171 culture group (91.49±2.68) ﹪and the MSCs co-culture group (78.11±2.35) ﹪. After amplification, the cells were cultured for 14 days, and colony formation were observed in all groups. Besides, cells in the UM171 group formed more erythroid cells and granulocytes than cells in the MSC co-culture group.

Conclusion

As feeder cells, umbilical cord MSCs can improvein vitroamplification of CD34⁺cells. UM171 can favorably maintain stemness of the cells in the amplification process. The optimal amplification can be achieved by combining umbilical cord MSCs and UM171.

Key words: Mesenchymal Stem Cells, CD34⁺, Hematopoietic stem cells, Fetal blood

meng Li, Hongxia Sheng, Yang Liu, Li Liao, Chong Tian, Peng Sun, Bin Zhang, Hu Chen. Effect of umbilical cord mesenchymal stem cells in combination with UM171 on amplification of cord blood CD34⁺ cells[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2017, 07(02): 93-100.

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Figures/Tables 10

References 16

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分组	例数	细胞总数	CD34⁺	CD133⁺（×10⁵）
对照组	6	3.76±0.43	3.57±0.36	0.28±0.07
UM171组	6	14.33±0.68	13.50±0.92	5.47±1.03
MSCs组	6	11.69±1.52	10.27±1.28	2.73±0.42
UM171及MSC共培养组	6	21.91±1.39	21.17±1.43	8.38±3.11
F值	?	274.38	272.43	26.82
P值	?	< 0.01	< 0.01	< 0.01

分组	例数	细胞总数	CD34⁺	CD133⁺（×10⁵）
对照组	6	3.76±0.43	3.57±0.36	0.28±0.07
UM171组	6	14.33±0.68	13.50±0.92	5.47±1.03
MSCs组	6	11.69±1.52	10.27±1.28	2.73±0.42
UM171及MSC共培养组	6	21.91±1.39	21.17±1.43	8.38±3.11
F值	?	274.38	272.43	26.82
P值	?	< 0.01	< 0.01	< 0.01

分组	例数	CD34⁺CD38^-	CD34⁺CD38⁺	CD34^-CD38⁺	CD34^-CD38^-	CD133⁺
对照组	6	72.44±12.47	22.70±1.88	2.88±0.39	1.91±0.37	7.61± 1.62
UM171组	6	87.67± 1.48	6.44±3.23	0.99±0.58	4.84±2.57	38.40± 8.24
MSCs组	6	78.11± 2.35	9.83±1.43	2.88±0.73	2.44±0.79	23.46± 3.29
UM171及MSC共培养组	6	91.49± 2.68	5.12±1.66	0.95±0.42	5.52±3.28	38.55±15.07
F值	?	86.87	82.48	24.35	35.13	16.90
P值	?	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01

分组	例数	CD34⁺CD38^-	CD34⁺CD38⁺	CD34^-CD38⁺	CD34^-CD38^-	CD133⁺
对照组	6	72.44±12.47	22.70±1.88	2.88±0.39	1.91±0.37	7.61± 1.62
UM171组	6	87.67± 1.48	6.44±3.23	0.99±0.58	4.84±2.57	38.40± 8.24
MSCs组	6	78.11± 2.35	9.83±1.43	2.88±0.73	2.44±0.79	23.46± 3.29
UM171及MSC共培养组	6	91.49± 2.68	5.12±1.66	0.95±0.42	5.52±3.28	38.55±15.07
F值	?	86.87	82.48	24.35	35.13	16.90
P值	?	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01

分组	例数	CFU-E	CFU-GM	CFU-GEMM
未扩增脐血组	6	28.33±4.32	84.17± 9.87	10.33±3.72
UM171组	6	35.17±7.99	73.83±16.58	13.00±2.83
MSCs组	6	19.17±3.60	51.00± 9.69	7.00±2.35
UM171及MSC共培养组	6	35.33±6.38	82.50± 7.87	13.50±3.83
F值	?	10.25	10.59	4.36
P值	?	< 0.01	0.02	< 0.01

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