Curative effect of human umbilical cord mesenchymal stem cells on ulcerative colitis mice

doi:10.3877/cma.j.issn.2095-1221.2017.02.005

Abstract

Abstract:

Objective

To investigate human umbilical cord derived mesenchymal stem cells (hUC-MSCs) transplantation for the treatment of ulcerative colitis (UC) in mice, and evaluated the effect of hUC-MSCs on tumor necrosis factor-alpha (TNF-α) and interleukin-8 (IL-8) production.

Methods

Forty eight male adult mice were randomly divided into three groups, namely normal control group, model control group and hUC-MSC group, with 16 mice in each group. UC was induced by drinking 5﹪dextran sulfate sodium (DSS) . The hUC-MSC group was treated by i.v. hUC-MSCs cells through tail vein. The disease activity index (DAI) was evaluated and TNF-αand IL-8 were detected by ELISA.t-test was used to determine statistical significance between the two groups, and ANOVA was used among the three groups.

Results

The DAI began to rise at the second day for the model control group and the hUC-MSCs group, which were (8.72±0.71) and (4.01±0.35) respectively at the end of the experiment, both higher than the normal control group (0.21±0.20,F= 1 300.66,P = 0.000) . The DAI were not different between the model control group and the hUC-MSCs group at the 4 days after induction of UC (7.95±0.71vs8.01±0.73,t=-0.236,P= 0.08153) . The DAI of the hUC-MSCs group began to decrease at the 5th treatment day and was lower than that of the model control group (7.23±0.82vs8.01±0.81,t=-2.707,P= 0.0111) . TNF-αlevels of the model control group (363.32±15.84) pg/ml and the hUC-MSCs group (375.52±16.21) pg/ml were similar (P> 0.05) , but higher than that of the normal group (112.14±11.23) pg/ml at the 7th day (F= 624.12,P= 0.000) ; IL-8 level of the model control group (65.32±10.12) pg/ml and the hUC-MSCs group (57.21±8.71) pg/ml were similar (P> 0.05) , but higher than that of the normal group (25.02±7.81) pg/ml at the 7th day (F= 34.29,P= 0.000) . Seven days after the second injection of stem cells (the end of experiment) , TNF-αlevel of the model control group (453.23±15.63) pg/ml and the hUC-MSCs group (276.84±17.91) pg/ml were higher than that of the normal group[ (134.32±12.31) pg/ml,F= 642.41,P= 0.000]; IL-8 level of the model control group (75.23±6.72) pg/ml and the hUC-MSCs group (39.12±4.42) pg/ml were higher than that of the normal group[ (27.12±5.91) pg/ml,F= 113.90,P= 0.000], but TNF-αand IL-8 levels of the hUC-MSCs group were lower than that of the model group (P< 0.05) .

Conclusion

hUC-MSCs may alleviate UC in DSS-treated mice via decreasing production of TNF-αand IL-8.

Key words: Colitis, ulcerative, Umbilical cord, Mesenchymal stem cells, Tumor necrosis factor-alpha, Interleukin-8

Junshan Zhai, Fang Li, Yamei Sun, Jiugang Song, Kai Wu, Yanmei Wang, Lin Zhang, Yi Yao, Nan Li. Curative effect of human umbilical cord mesenchymal stem cells on ulcerative colitis mice[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2017, 07(02): 87-92.

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Figures/Tables 6

References 14

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体重降低率^a	大便性状	血便	评分
无变化	成形	正常	0
1﹪~ 5﹪	半成型，不黏附肛门	无明显可见出血	1
5﹪~ 10﹪	半成型，黏附肛门	有明显可见出血	2
10﹪~ 20﹪	水样	血便	3
20﹪以上	—	—	4

体重降低率^a	大便性状	血便	评分
无变化	成形	正常	0
1﹪~ 5﹪	半成型，不黏附肛门	无明显可见出血	1
5﹪~ 10﹪	半成型，黏附肛门	有明显可见出血	2
10﹪~ 20﹪	水样	血便	3
20﹪以上	—	—	4

分组	第1天	第2天	第3天	第4天	第5天	第6天	第7天	第8天	第9天
正常对照组	0.28±0.07	0.31±0.23	0.35±0.21	0.29±0.22	0.21±0.19	0.34±0.23	0.43±0.26	0.37±0.21	0.39±0.25
模型对照组	0.26±0.03	2.01±0.07^a	3.91±0.12^a	4.12±0.23^a	5.13±0.34^a	6.08±0.44^a	6.67±0.51^a	7.23±0.61^a	7.47±0.71^a
治疗组	0.31±0.02	1.68±0.06^a	3.47±0.14^a	4.33±0.25^a	5.52±0.31^a	6.43±0.46^a	6.55±0.48^a	7.12±0.59^a	7.63±0.67^a
F值	0.00	635.28	2 781.59	1 515.73	1 696.68	1 225.20	1 112.72	969.61	808.03
P值	> 0.05	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01
分组	第10天	第11天	第12天	第13天	第14天	第15天	第16天	第17天
正常对照组	0.44±0.24	0.16±0.12	0.19±0.12	0.22±0.16	0.35±0.25	0.32±0.23	0.24±0.22	0.21±0.20
模型对照组	7.65±0.67^a	7.95±0.71^a	8.01±0.81^a	8.23±0.82^a	8.43±0.79^a	8.54±0.68^a	8.61±0.65^a	8.72±0.71^a
治疗组	7.83±0.65^a	8.01±0.73^a	7.23±0.82^ab	6.91±0.61^ab	5.53±0.54^ab	5.01±0.43^ab	4.23±0.37^ab	4.01±0.35^ab
F值	918.22	930.65	663.18	827.28	822.15	1 165.67	1 384.16	1 300.66
P值	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01

分组	第1天	第2天	第3天	第4天	第5天	第6天	第7天	第8天	第9天
正常对照组	0.28±0.07	0.31±0.23	0.35±0.21	0.29±0.22	0.21±0.19	0.34±0.23	0.43±0.26	0.37±0.21	0.39±0.25
模型对照组	0.26±0.03	2.01±0.07^a	3.91±0.12^a	4.12±0.23^a	5.13±0.34^a	6.08±0.44^a	6.67±0.51^a	7.23±0.61^a	7.47±0.71^a
治疗组	0.31±0.02	1.68±0.06^a	3.47±0.14^a	4.33±0.25^a	5.52±0.31^a	6.43±0.46^a	6.55±0.48^a	7.12±0.59^a	7.63±0.67^a
F值	0.00	635.28	2 781.59	1 515.73	1 696.68	1 225.20	1 112.72	969.61	808.03
P值	> 0.05	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01
分组	第10天	第11天	第12天	第13天	第14天	第15天	第16天	第17天
正常对照组	0.44±0.24	0.16±0.12	0.19±0.12	0.22±0.16	0.35±0.25	0.32±0.23	0.24±0.22	0.21±0.20
模型对照组	7.65±0.67^a	7.95±0.71^a	8.01±0.81^a	8.23±0.82^a	8.43±0.79^a	8.54±0.68^a	8.61±0.65^a	8.72±0.71^a
治疗组	7.83±0.65^a	8.01±0.73^a	7.23±0.82^ab	6.91±0.61^ab	5.53±0.54^ab	5.01±0.43^ab	4.23±0.37^ab	4.01±0.35^ab
F值	918.22	930.65	663.18	827.28	822.15	1 165.67	1 384.16	1 300.66
P值	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01

分组	只数	TNF-α（pg/ml）		IL-8（pg/ml）		CRP（mg/ml）
分组	只数	造模第7天	实验结束	造模第7天	实验结束	造模第7天	实验结束
空白对照组	6	112.14±11.23	134.32±12.31	25.02± 7.81	27.12±5.91	0.35±0.12	0.41±0.13
模型对照组	6	363.32±15.84^a	453.23±15.63^a	65.32±10.12^a	75.23±6.72^a	1.57±0.18^a	1.43±0.16^a
治疗组	6	375.52±16.21^a	276.84±17.91^ab	57.21± 8.71^a	39.12±4.42^ab	1.62±0.15^a	0.88±0.21^ab
F值	?	624.12	642.41	34.29	113.90	134.36	54.17
P值	?	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01

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