Comparisons of two isolation methods for exosomes from human umbilical cord mesenchymal stem cells

doi:10.3877/cma.j.issn.2095-1221.2017.02.004

Abstract

Abstract:

Objective

To explore the best method of separating exosomes secreted by human umbilical cord mesenchymal stem cells (huMSCS) .

Methods

Exosomes were collected from huMSCS in the serum-free medium culture system by either ultracentrifugation or precipitation methods. Western Blot was used to detect TAPA1, CD63, HSP70, and HSP90 of exosomes. Tansmission electron microscope was used to observe exosome morphology, and proteome of exosomes isolated via the two methods was compared.

Results

Exosomes isolated via ultracentrifigation were around 100 nm in diameter under transmission electron microscopy and were positive for CD63, HSP70, and HSP90, while negative for TAPA1; exosomes isolated via 101 Kit were around 30 nm in diameter and were positive only for HSP90. High-resolution mass spectrometry (HRMS) revealed 169 proteins in exosomes isolated via ultracentrifigation while only 102 proteins were identified in exosomes isolated via 101 Kit. Comparative analysis dates from HRMS indicated that there were 79 proteins shared by exosomes isolated by the two methods.

Conclusion

Exosomes isolated by ultracentrifugation and precipitation are different in biomarkers and proteome profile, and ultracentrifugation method is superior to precipitation method.

Key words: Umbilical Cord, Mesenchymal Stem Cells, Exosomes, Isolation, Identification

Miyang Liu-Gao, Xinghu Pan, Falian He, Rong Lu, Jufen Liu, Jie He, Hongyang Wang. Comparisons of two isolation methods for exosomes from human umbilical cord mesenchymal stem cells[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2017, 07(02): 81-86,f3-f8.

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Figures/Tables 7

References 16

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UNIPROT_ACCESSION	NAME
P27824	calnexin(CANX) Homo sapiens
P04217	alpha-1-B glycoprotein(A1BG)
P68104	eukaryotic translation elongation factor 1 alpha 1(EEF1A1)
P61626	lysozyme(LYZ)
P22735	transglutaminase 1(TGM1)
P22792	carboxypeptidase N subunit 2(CPN2)
P07585	decorin(DCN)
P01011	serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3(SERPINA3)
Q5T749	keratinocyte proline rich protein(KPRP)
P61769	beta-2-microglobulin(B2M)
P00450	ceruloplasmin (ferroxidase)(CP)
P29966	myristoylated alanine rich protein kinase C substrate(MARCKS)
P25311	alpha-2-glycoprotein 1, zinc-binding(AZGP1)
P0CG05	immunoglobulin lambda constant 2 (Kern-Oz- marker)(IGLC2)
P12883	myosin, heavy chain 7, cardiac muscle, beta(MYH7)
Q16610	extracellular matrix protein 1(ECM1)
P07996	thrombospondin 1(THBS1)
P27482	calmodulin like 3(CALML3)
P04792	heat shock protein family B (small) member 1(HSPB1)
Q9H1J7	wingless-type MMTV integration site family member 5B(WNT5B)
P01033	TIMP metallopeptidase inhibitor 1(TIMP1)
P35542	serum amyloid A4, constitutive(SAA4)
P0C0L5	complement component 4B (Chido blood group), copy 2(C4B_2)
P12235	solute carrier family 25 member 4(SLC25A4)
Q8N1N4	keratin 78, type II(KRT78)
Q66K66	transmembrane protein 198(TMEM198)
Q5D862	filaggrin family member 2(FLG2)
P11021	heat shock protein family A (Hsp70) member 5(HSPA5)
P02788	lactotransferrin(LTF)
Q86YZ3	hornerin(HRNR)
P31151	S100 calcium binding protein A7(S100A7)
P51884	lumican(LUM)
P07355	annexin A2(ANXA2)
P05109	S100 calcium binding protein A8(S100A8)
P02654	apolipoprotein C-I(APOC1)
P06307	cholecystokinin(CCK)
P02652	apolipoprotein A-II(APOA2)
P21741	midkine (neurite growth-promoting factor 2)(MDK)
P68871	hemoglobin subunit beta(HBB)
P23490	loricrin(LOR)
P05090	apolipoprotein D(APOD)
P02655	apolipoprotein C-II(APOC2)
P12109	collagen type VI alpha 1(COL6A1)
P68371	tubulin beta 4B class IVb(TUBB4B)
P15924	desmoplakin(DSP)
P12110	collagen type VI alpha 2(COL6A2)
B4DZ36	attractin(ATRN)
P12111	collagen type VI alpha 3(COL6A3)
Q02413	desmoglein 1(DSG1)
P02647	apolipoprotein A-I(APOA1)
P02458	collagen type II alpha 1(COL2A1)
P02461	collagen type III alpha 1(COL3A1)
P20929	nebulin(NEB)
Q08554	desmocollin 1(DSC1)
P31944	caspase 14(CASP14)
P05543	serpin peptidase inhibitor, clade A , member 7(SERPINA7)
P62807	histone cluster 1, H2bd(HIST1H2BD)
P02753	retinol binding protein 4(RBP4)
Q16270	insulin like growth factor binding protein 7(IGFBP7)
P05089	arginase 1(ARG1)
P06276	butyrylcholinesterase(BCHE)
P35611	adducin 1(ADD1)
P69905	hemoglobin subunit alpha 1(HBA1)
P27487	dipeptidyl peptidase 4(DPP4)
P25788	proteasome subunit alpha 3(PSMA3)
P01834	immunoglobulin kappa constant(IGKC)
P00738	haptoglobin(HP)
Q6KB66	keratin 80, type II(KRT80)
P62987	ubiquitin A-52 residue ribosomal protein fusion product 1(UBA52)
P43251	biotinidase(BTD)
P14209	CD99 molecule(CD99)
P10599	thioredoxin(TXN)
P09486	secreted protein acidic and cysteine rich(SPARC)
P08697	serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2(SERPINF2)
P20930	filaggrin(FLG)
Q9Y3U8	ribosomal protein L36(RPL36)
P13667	protein disulfide isomerase family A member 4(PDIA4)
O95445	apolipoprotein M(APOM)
P05556	integrin subunit beta 1(ITGB1)
P02763	orosomucoid 1(ORM1)
P02766	transthyretin(TTR)
P04406	glyceraldehyde-3-phosphate dehydrogenase(GAPDH)
P19652	orosomucoid 2(ORM2)
P01040	cystatin A(CSTA)
Q96C01	family with sequence similarity 136 member A(FAM136A)
P08571	CD14 molecule(CD14)
P02751	fibronectin 1(FN1)
Q08380	lectin, galactoside-binding, soluble, 3 binding protein(LGALS3BP)
P23396	ribosomal protein S3(RPS3)
P80108	glycosylphosphatidylinositol specific phospholipase D1(GPLD1)
P62899	ribosomal protein L31(RPL31)
Q13835	plakophilin 1(PKP1)
P62805	histone cluster 4, H4(HIST4H4)
Q6EMK4	vasorin(VASN)
P62424	ribosomal protein L7a(RPL7A)
P43652	afamin(AFM)
P22531	small proline rich protein 2E(SPRR2E)
P14618	pyruvate kinase, muscle(PKM)
P07339	cathepsin D(CTSD)
P81605	dermcidin(DCD)
Q5T750	chromosome 1 open reading frame 68(C1orf68)
P08123	collagen type I alpha 2(COL1A2)
P02452	collagen type I alpha 1(COL1A1)
Q14847	LIM and SH3 protein 1(LASP1)
P22528	small proline rich protein 1B(SPRR1B)
Q7Z7G0	ABI family member 3 binding protein(ABI3BP)
P46779	ribosomal protein L28(RPL28)
P13637	ATPase Na+/K+ transporting subunit alpha 3(ATP1A3)
Q14677	clathrin interactor 1(CLINT1)
P08727	keratin 19, type I(KRT19)
P02765	alpha-2-HS-glycoprotein(AHSG)
Q96PD5	peptidoglycan recognition protein 2(PGLYRP2)
Q15758	solute carrier family 1 member 5(SLC1A5)
P27090	transforming growth factor, beta 2(Tgfb2)
Q04695	keratin 17, type I(KRT17)
Q96KN2	carnosine dipeptidase 1 (metallopeptidase M20 family)(CNDP1)
P01308	insulin(INS)
P01008	serpin peptidase inhibitor, clade C (antithrombin), member 1(SERPINC1)
P02768	albumin(ALB)
Q9NY65	enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase(EHHADH)
P04259	keratin 6B, type II(KRT6B)
P13647	keratin 5, type II(KRT5)
P10909	clusterin(CLU)
P19013	keratin 4, type II(KRT4)
P04264	keratin 1, type II(KRT1)
P35908	keratin 2, type II(KRT2)
P26022	pentraxin 3(PTX3)
P48509	CD151 molecule (Raph blood group)(CD151)
P51164	ATPase H+/K+ transporting beta subunit(ATP4B)
Q01469	fatty acid binding protein 5(FABP5)
P04075	aldolase, fructose-bisphosphate A(ALDOA)
P01009	serpin peptidase inhibitor, clade A , member 1(SERPINA1)
P09919	colony stimulating factor 3(CSF3)
P08779	keratin 16, type I(KRT16)
Q9UNN8	protein C receptor(PROCR)
Q96DR8	mucin like 1(MUCL1)
P00491	purine nucleoside phosphorylase(PNP)
P07093	serpin peptidase inhibitor, clade E , member 2(SERPINE2)
Q9HCY8	S100 calcium binding protein A14(S100A14)
P67936	tropomyosin 4(TPM4)
P16070	CD44 molecule (Indian blood group)(CD44)
P62249	ribosomal protein S16(RPS16)
P08962	CD63 molecule(CD63)
P13645	keratin 10, type I(KRT10)
P02786	transferrin receptor(TFRC)
P35527	keratin 9, type I(KRT9)
P02774	group-specific component (vitamin D binding protein)(GC)
P05787	keratin 8, type II(KRT8)
Q00610	clathrin heavy chain(CLTC)

UNIPROT_ACCESSION	NAME
P27824	calnexin(CANX) Homo sapiens
P04217	alpha-1-B glycoprotein(A1BG)
P68104	eukaryotic translation elongation factor 1 alpha 1(EEF1A1)
P61626	lysozyme(LYZ)
P22735	transglutaminase 1(TGM1)
P22792	carboxypeptidase N subunit 2(CPN2)
P07585	decorin(DCN)
P01011	serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3(SERPINA3)
Q5T749	keratinocyte proline rich protein(KPRP)
P61769	beta-2-microglobulin(B2M)
P00450	ceruloplasmin (ferroxidase)(CP)
P29966	myristoylated alanine rich protein kinase C substrate(MARCKS)
P25311	alpha-2-glycoprotein 1, zinc-binding(AZGP1)
P0CG05	immunoglobulin lambda constant 2 (Kern-Oz- marker)(IGLC2)
P12883	myosin, heavy chain 7, cardiac muscle, beta(MYH7)
Q16610	extracellular matrix protein 1(ECM1)
P07996	thrombospondin 1(THBS1)
P27482	calmodulin like 3(CALML3)
P04792	heat shock protein family B (small) member 1(HSPB1)
Q9H1J7	wingless-type MMTV integration site family member 5B(WNT5B)
P01033	TIMP metallopeptidase inhibitor 1(TIMP1)
P35542	serum amyloid A4, constitutive(SAA4)
P0C0L5	complement component 4B (Chido blood group), copy 2(C4B_2)
P12235	solute carrier family 25 member 4(SLC25A4)
Q8N1N4	keratin 78, type II(KRT78)
Q66K66	transmembrane protein 198(TMEM198)
Q5D862	filaggrin family member 2(FLG2)
P11021	heat shock protein family A (Hsp70) member 5(HSPA5)
P02788	lactotransferrin(LTF)
Q86YZ3	hornerin(HRNR)
P31151	S100 calcium binding protein A7(S100A7)
P51884	lumican(LUM)
P07355	annexin A2(ANXA2)
P05109	S100 calcium binding protein A8(S100A8)
P02654	apolipoprotein C-I(APOC1)
P06307	cholecystokinin(CCK)
P02652	apolipoprotein A-II(APOA2)
P21741	midkine (neurite growth-promoting factor 2)(MDK)
P68871	hemoglobin subunit beta(HBB)
P23490	loricrin(LOR)
P05090	apolipoprotein D(APOD)
P02655	apolipoprotein C-II(APOC2)
P12109	collagen type VI alpha 1(COL6A1)
P68371	tubulin beta 4B class IVb(TUBB4B)
P15924	desmoplakin(DSP)
P12110	collagen type VI alpha 2(COL6A2)
B4DZ36	attractin(ATRN)
P12111	collagen type VI alpha 3(COL6A3)
Q02413	desmoglein 1(DSG1)
P02647	apolipoprotein A-I(APOA1)
P02458	collagen type II alpha 1(COL2A1)
P02461	collagen type III alpha 1(COL3A1)
P20929	nebulin(NEB)
Q08554	desmocollin 1(DSC1)
P31944	caspase 14(CASP14)
P05543	serpin peptidase inhibitor, clade A , member 7(SERPINA7)
P62807	histone cluster 1, H2bd(HIST1H2BD)
P02753	retinol binding protein 4(RBP4)
Q16270	insulin like growth factor binding protein 7(IGFBP7)
P05089	arginase 1(ARG1)
P06276	butyrylcholinesterase(BCHE)
P35611	adducin 1(ADD1)
P69905	hemoglobin subunit alpha 1(HBA1)
P27487	dipeptidyl peptidase 4(DPP4)
P25788	proteasome subunit alpha 3(PSMA3)
P01834	immunoglobulin kappa constant(IGKC)
P00738	haptoglobin(HP)
Q6KB66	keratin 80, type II(KRT80)
P62987	ubiquitin A-52 residue ribosomal protein fusion product 1(UBA52)
P43251	biotinidase(BTD)
P14209	CD99 molecule(CD99)
P10599	thioredoxin(TXN)
P09486	secreted protein acidic and cysteine rich(SPARC)
P08697	serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2(SERPINF2)
P20930	filaggrin(FLG)
Q9Y3U8	ribosomal protein L36(RPL36)
P13667	protein disulfide isomerase family A member 4(PDIA4)
O95445	apolipoprotein M(APOM)
P05556	integrin subunit beta 1(ITGB1)
P02763	orosomucoid 1(ORM1)
P02766	transthyretin(TTR)
P04406	glyceraldehyde-3-phosphate dehydrogenase(GAPDH)
P19652	orosomucoid 2(ORM2)
P01040	cystatin A(CSTA)
Q96C01	family with sequence similarity 136 member A(FAM136A)
P08571	CD14 molecule(CD14)
P02751	fibronectin 1(FN1)
Q08380	lectin, galactoside-binding, soluble, 3 binding protein(LGALS3BP)
P23396	ribosomal protein S3(RPS3)
P80108	glycosylphosphatidylinositol specific phospholipase D1(GPLD1)
P62899	ribosomal protein L31(RPL31)
Q13835	plakophilin 1(PKP1)
P62805	histone cluster 4, H4(HIST4H4)
Q6EMK4	vasorin(VASN)
P62424	ribosomal protein L7a(RPL7A)
P43652	afamin(AFM)
P22531	small proline rich protein 2E(SPRR2E)
P14618	pyruvate kinase, muscle(PKM)
P07339	cathepsin D(CTSD)
P81605	dermcidin(DCD)
Q5T750	chromosome 1 open reading frame 68(C1orf68)
P08123	collagen type I alpha 2(COL1A2)
P02452	collagen type I alpha 1(COL1A1)
Q14847	LIM and SH3 protein 1(LASP1)
P22528	small proline rich protein 1B(SPRR1B)
Q7Z7G0	ABI family member 3 binding protein(ABI3BP)
P46779	ribosomal protein L28(RPL28)
P13637	ATPase Na+/K+ transporting subunit alpha 3(ATP1A3)
Q14677	clathrin interactor 1(CLINT1)
P08727	keratin 19, type I(KRT19)
P02765	alpha-2-HS-glycoprotein(AHSG)
Q96PD5	peptidoglycan recognition protein 2(PGLYRP2)
Q15758	solute carrier family 1 member 5(SLC1A5)
P27090	transforming growth factor, beta 2(Tgfb2)
Q04695	keratin 17, type I(KRT17)
Q96KN2	carnosine dipeptidase 1 (metallopeptidase M20 family)(CNDP1)
P01308	insulin(INS)
P01008	serpin peptidase inhibitor, clade C (antithrombin), member 1(SERPINC1)
P02768	albumin(ALB)
Q9NY65	enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase(EHHADH)
P04259	keratin 6B, type II(KRT6B)
P13647	keratin 5, type II(KRT5)
P10909	clusterin(CLU)
P19013	keratin 4, type II(KRT4)
P04264	keratin 1, type II(KRT1)
P35908	keratin 2, type II(KRT2)
P26022	pentraxin 3(PTX3)
P48509	CD151 molecule (Raph blood group)(CD151)
P51164	ATPase H+/K+ transporting beta subunit(ATP4B)
Q01469	fatty acid binding protein 5(FABP5)
P04075	aldolase, fructose-bisphosphate A(ALDOA)
P01009	serpin peptidase inhibitor, clade A , member 1(SERPINA1)
P09919	colony stimulating factor 3(CSF3)
P08779	keratin 16, type I(KRT16)
Q9UNN8	protein C receptor(PROCR)
Q96DR8	mucin like 1(MUCL1)
P00491	purine nucleoside phosphorylase(PNP)
P07093	serpin peptidase inhibitor, clade E , member 2(SERPINE2)
Q9HCY8	S100 calcium binding protein A14(S100A14)
P67936	tropomyosin 4(TPM4)
P16070	CD44 molecule (Indian blood group)(CD44)
P62249	ribosomal protein S16(RPS16)
P08962	CD63 molecule(CD63)
P13645	keratin 10, type I(KRT10)
P02786	transferrin receptor(TFRC)
P35527	keratin 9, type I(KRT9)
P02774	group-specific component (vitamin D binding protein)(GC)
P05787	keratin 8, type II(KRT8)
Q00610	clathrin heavy chain(CLTC)

UNIPROT_ACCESSION	Name
P43251	biotinidase(BTD)
P10599	thioredoxin(TXN
P04217	alpha-1-B glycoprotein(A1BG)
P09486	secreted protein acidic and cysteine rich(SPARC)
P68104	eukaryotic translation elongation factor 1 alpha 1(EEF1A1)
P08697	serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2(SERPINF2)
P07585	decorin(DCN)
P20930	filaggrin(FLG)
P01011	serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3(SERPINA3)
Q5T749	keratinocyte proline rich protein(KPRP)
P02763	orosomucoid 1(ORM1)
P02766	transthyretin(TTR)
P19652	orosomucoid 2(ORM2)
P0CG05	immunoglobulin lambda constant 2 (Kern-Oz- marker)(IGLC2)
P25311	alpha-2-glycoprotein 1, zinc-binding(AZGP1)
P27482	calmodulin like 3(CALML3)
Q08380	lectin, galactoside-binding, soluble, 3 binding protein(LGALS3BP)
P07996	thrombospondin 1(THBS1)
P22531	small proline rich protein 2E(SPRR2E)
P43652	afamin(AFM)
P01033	TIMP metallopeptidase inhibitor 1(TIMP1)
P0C0L5	complement component 4B (Chido blood group), copy 2(C4B_2)
P81605	dermcidin(DCD)
Q8N1N4	keratin 78, type II(KRT78)
Q66K66	transmembrane protein 198(TMEM198)
P08123	collagen type I alpha 2(COL1A2) Homo sapiens
Q5D862	filaggrin family member 2(FLG2)
P02452	collagen type I alpha 1(COL1A1)
P02788	lactotransferrin(LTF)
Q86YZ3	hornerin(HRNR)
P31151	S100 calcium binding protein A7(S100A7)
P51884	lumican(LUM)
P05109	S100 calcium binding protein A8(S100A8)
P06307	cholecystokinin(CCK)
P08727	keratin 19, type I(KRT19)
P02652	apolipoprotein A-II(APOA2)
P02765	alpha-2-HS-glycoprotein(AHSG)
P68871	hemoglobin subunit beta(HBB)
Q96PD5	peptidoglycan recognition protein 2(PGLYRP2)
P27090	transforming growth factor, beta 2(Tgfb2)
P05090	apolipoprotein D(APOD)
Q04695	keratin 17, type I(KRT17)
P01308	insulin(INS)
P15924	desmoplakin(DSP)
B4DZ36	attractin(ATRN)
P02768	albumin(ALB)
P02458	collagen type II alpha 1(COL2A1)
P02461	collagen type III alpha 1(COL3A1)
P04259	keratin 6B, type II(KRT6B)
P05543	serpin peptidase inhibitor, clade A , member 7(SERPINA7)
P31944	caspase 14(CASP14)
P02753	retinol binding protein 4(RBP4)
P13647	keratin 5, type II(KRT5)
Q16270	insulin like growth factor binding protein 7(IGFBP7)
P19013	keratin 4, type II(KRT4)
P04264	keratin 1, type II(KRT1
P35908	keratin 2, type II(KRT2)
P26022	pentraxin 3(PTX3)
P05089	arginase 1(ARG1)
P09919	colony stimulating factor 3(CSF3)
P35611	adducin 1(ADD1)
P08779	keratin 16, type I(KRT16)
P69905	hemoglobin subunit alpha 1(HBA1)
Q96DR8	mucin like 1(MUCL1)
P01834	immunoglobulin kappa constant(IGKC)
P67936	tropomyosin 4(TPM4)
Q6KB66	keratin 80, type II(KRT80)
P00738	haptoglobin(HP)
P13645	keratin 10, type I(KRT10)
P02786	transferrin receptor(TFRC)
P35527	keratin 9, type I(KRT9)
P02774	group-specific component (vitamin D binding protein)(GC)
P05787	keratin 8, type II(KRT8)
P62987	ubiquitin A-52 residue ribosomal protein fusion product 1(UBA52)

UNIPROT_ACCESSION	Name
P43251	biotinidase(BTD)
P10599	thioredoxin(TXN
P04217	alpha-1-B glycoprotein(A1BG)
P09486	secreted protein acidic and cysteine rich(SPARC)
P68104	eukaryotic translation elongation factor 1 alpha 1(EEF1A1)
P08697	serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2(SERPINF2)
P07585	decorin(DCN)
P20930	filaggrin(FLG)
P01011	serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3(SERPINA3)
Q5T749	keratinocyte proline rich protein(KPRP)
P02763	orosomucoid 1(ORM1)
P02766	transthyretin(TTR)
P19652	orosomucoid 2(ORM2)
P0CG05	immunoglobulin lambda constant 2 (Kern-Oz- marker)(IGLC2)
P25311	alpha-2-glycoprotein 1, zinc-binding(AZGP1)
P27482	calmodulin like 3(CALML3)
Q08380	lectin, galactoside-binding, soluble, 3 binding protein(LGALS3BP)
P07996	thrombospondin 1(THBS1)
P22531	small proline rich protein 2E(SPRR2E)
P43652	afamin(AFM)
P01033	TIMP metallopeptidase inhibitor 1(TIMP1)
P0C0L5	complement component 4B (Chido blood group), copy 2(C4B_2)
P81605	dermcidin(DCD)
Q8N1N4	keratin 78, type II(KRT78)
Q66K66	transmembrane protein 198(TMEM198)
P08123	collagen type I alpha 2(COL1A2) Homo sapiens
Q5D862	filaggrin family member 2(FLG2)
P02452	collagen type I alpha 1(COL1A1)
P02788	lactotransferrin(LTF)
Q86YZ3	hornerin(HRNR)
P31151	S100 calcium binding protein A7(S100A7)
P51884	lumican(LUM)
P05109	S100 calcium binding protein A8(S100A8)
P06307	cholecystokinin(CCK)
P08727	keratin 19, type I(KRT19)
P02652	apolipoprotein A-II(APOA2)
P02765	alpha-2-HS-glycoprotein(AHSG)
P68871	hemoglobin subunit beta(HBB)
Q96PD5	peptidoglycan recognition protein 2(PGLYRP2)
P27090	transforming growth factor, beta 2(Tgfb2)
P05090	apolipoprotein D(APOD)
Q04695	keratin 17, type I(KRT17)
P01308	insulin(INS)
P15924	desmoplakin(DSP)
B4DZ36	attractin(ATRN)
P02768	albumin(ALB)
P02458	collagen type II alpha 1(COL2A1)
P02461	collagen type III alpha 1(COL3A1)
P04259	keratin 6B, type II(KRT6B)
P05543	serpin peptidase inhibitor, clade A , member 7(SERPINA7)
P31944	caspase 14(CASP14)
P02753	retinol binding protein 4(RBP4)
P13647	keratin 5, type II(KRT5)
Q16270	insulin like growth factor binding protein 7(IGFBP7)
P19013	keratin 4, type II(KRT4)
P04264	keratin 1, type II(KRT1
P35908	keratin 2, type II(KRT2)
P26022	pentraxin 3(PTX3)
P05089	arginase 1(ARG1)
P09919	colony stimulating factor 3(CSF3)
P35611	adducin 1(ADD1)
P08779	keratin 16, type I(KRT16)
P69905	hemoglobin subunit alpha 1(HBA1)
Q96DR8	mucin like 1(MUCL1)
P01834	immunoglobulin kappa constant(IGKC)
P67936	tropomyosin 4(TPM4)
Q6KB66	keratin 80, type II(KRT80)
P00738	haptoglobin(HP)
P13645	keratin 10, type I(KRT10)
P02786	transferrin receptor(TFRC)
P35527	keratin 9, type I(KRT9)
P02774	group-specific component (vitamin D binding protein)(GC)
P05787	keratin 8, type II(KRT8)
P62987	ubiquitin A-52 residue ribosomal protein fusion product 1(UBA52)

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