探讨星形胶质瘤细胞来源的外泌体中microRNA-1246（miRNA-1246）是否作用于星形胶质瘤细胞，促进其增殖与侵袭。

实验分为对照组、miRNA-1246抑制剂组与miRNA-1246模拟物组，各组设6个复孔。首先从患者血液中分离外泌体并鉴定其成分。通过基质胶包被的Transwell小室实验检测星形胶质瘤细胞在miRNA-1246作用下侵袭能力的变化，CCK-8实验检测细胞增殖能力。利用荧光素酶报告基因验证miRNA-1246是否靶向细胞黏附分子1（CADM1）基因。最后通过Western Blot实验与RT-qPCR实验检测癌症组织中CADM1蛋白水平的含量并分析其与胶质瘤的关系。采用方差分析和t检验进行统计学分析。

恶性胶质瘤患者血液循环外泌体中miRNA-1246的含量为2.83±1.70，高于对照组1.00±0.50，差异具有统计学意义（t?=?6.044，P?=?0.026）。转染miRNA-1246抑制剂后细胞CADM1蛋白水平为1.79±0.17，高于对照组1.00±0.09（t?=?4.576，P?=?0.017），细胞侵袭数量为（48.40±5.90）个，低于对照组96.50±6.70，而转染miRNA-1246模拟物后细胞侵袭数量为（123.20±9.80）个，高于对照组（96.50±6.70）个（t?=?5.258，P?=?0.002）。CCK-8实验中转染miRNA-1246抑制剂组A450值为0.49±0.08，低于对照组0.76±0.06，而转染miRNA-1246模拟物组A450值为1.03±0.09，显著升高（F?=?33.82，P?=?0.005）。荧光素酶报告实验表明细胞转染miR-?1246模拟物后荧光强度为4.98±1.86，低于对照组10.34±2.60（t?=?7.235，P?=?0.006），而CADM1-Mut组内之间比较差异无统计学意义。

胶质瘤细胞外泌体中的miRNA-1246可通过靶向CADM1基因抑制蛋白表达，促进胶质瘤细胞的增殖与转移，提示循环外泌体中的miRNA-1246可作为恶性胶质瘤诊断与治疗的潜在靶点。

To explore whether exosomal miRNA-1246 promotes proliferation and invasion acts of astroglioma cells.

Exosomes were isolated from the patient's blood and their components were identified. The changes in the invasiveness of astroglioma cells under the action of microrna-1246 were examined by transwell chamber test. Luciferase reporter assay was used to verify whether cell adhesion molecule 1 (CADM1) is the target gene of miRNA-1246. Finally, Western Blot and RT-qPCR were used to detect CADM1 protein in cancer tissues and analyzed its relationship with glioma. ANOVA and t test were used for statistical analysis.

The contents of microRNA-1246 in the exosomes of blood circulation in patients with malignant glioma (2.83±1.70) were significantly increased compared with control group (1.00±0.50) (P?<?0.05). Cell invasion in the miRNA-1246 inhibitor group (48.40±5.90) decreased compared with the control group (96.50±6.70). In the luciferase reporter gene assay, fluorescence intensity of the miRNA-1246 mimic group (4.98±1.86) significantly decreased compared with the control group (10.34±2.60) (P?<?0.05). In vitro experiments showed that microRNA-1246 can affect the expression of CADM1 gene, and thereby affect the proliferation and migration of glioma cells.

MicroRNA-1246 in glioma cell derived exosomes can promote the proliferation and metastasis of glioma cells by inhibiting CADM1 expression, indicating that microRNA-1246 can be used as a potential target for the diagnosis and treatment of malignant glioma.