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中华细胞与干细胞杂志(电子版)

中华细胞与干细胞杂志(电子版) ›› 2017, Vol. 07 ›› Issue (02) : 117 -123. doi: 10.3877/cma.j.issn.2095-1221.2017.02.010

所属专题： 文献；

综述

毛囊源性黑色素细胞的生物学特性及其体外培养技术研究进展
樊蕊蕊1, 刘菲琳2, 郝德顺3, 张琪4, 张莹瑶4, 郑彤4, 李士伦5, 王鸿艳6, 戚良晨7, 刘晋宇4,†()   
  1. 1. 130021　长春，吉林大学公共卫生学院毒理教研室；353000　南平，福建医科大学附属南平市第一医院病理科
    2. 130021　长春，吉林大学附属第二医院院眼科
    3. 130021　长春，吉林大学公共卫生学院基础医学院病理教研室
    4. 130021　长春，吉林大学公共卫生学院毒理教研室
    5. 136500　四平，梨树县第一人民医院肿瘤科
    6. 130021　长春，武警吉林总队医院妇产科
    7. 130021　长春，吉林大学中日联谊医院胸外科
  • 收稿日期:2016-10-07 出版日期:2017-04-01
  • 通信作者: 刘晋宇

Progress in biology and in vitro culture of hair follicle-derived melanocytes

Ruirui Fan1, Feilin Liu2, Deshun Hao3, Qi Zhang4, Yingyao Zhang4, Tong Zheng4, Shilun Li5, Hongyan Wang6, Liangchen Qi7, Jinyu Liu4,†()   

  1. 1. Department of Toxicology, School of Public Health, Jilin University, Changchun 130021, China; Department of Pathology, The First Hospital of Nanping, Nanping 353000, China
    2. The Second Affiliated Hospital of Jilin University, Ophthalmic hospital, Changchun 130021, China
    3. Department of Pathology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China
    4. Department of Toxicology, School of Public Health, Jilin University, Changchun 130021, China
    5. Department of Oncology, Lishu First People's Hospital, Siping 136500, China
    6. Department of Obstetrics and Gynecology, Jilin General Hospital of Armed Police Force, Changchun 130021, China
    7. Department of Thoracic Surgery, China-Japan Friendship Hospital, Jilin University, Changchun 130021, China
  • Received:2016-10-07 Published:2017-04-01
  • Corresponding author: Jinyu Liu
  • About author:
    Corresponding author: Liu Jinyu, Email:
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樊蕊蕊, 刘菲琳, 郝德顺, 张琪, 张莹瑶, 郑彤, 李士伦, 王鸿艳, 戚良晨, 刘晋宇. 毛囊源性黑色素细胞的生物学特性及其体外培养技术研究进展[J]. 中华细胞与干细胞杂志(电子版), 2017, 07(02): 117-123.

Ruirui Fan, Feilin Liu, Deshun Hao, Qi Zhang, Yingyao Zhang, Tong Zheng, Shilun Li, Hongyan Wang, Liangchen Qi, Jinyu Liu. Progress in biology and in vitro culture of hair follicle-derived melanocytes[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2017, 07(02): 117-123.

黑色素细胞（MC），是脊椎动物皮肤、毛发着色的主要细胞，主要存在于表皮基底层，这部分细胞约占基底细胞的10﹪；另外还有少量存在于毛囊、内耳血管纹、眼睛脉络膜等，它的功能或者数量的突变，会导致一系列难治性脱色素性疾病。目前针对表皮来源的MC研究已经很多，体外培养技术也比较成熟，但表皮MC不能为毛发着色，毛囊源性MC能够合成黑色素，且移植后能够归巢到皮肤，从而为色素障碍性疾病的治疗提供理论基础和实验依据。本文就毛囊源性MC的生物学特性，鉴定方法，以及体外培养技术的研究进展进行了综述。

关键词: 毛囊, 黑素细胞, 生物学, 细胞培养技术

Melanocytes are main cells in the vertebrate for staining skin and fair and locate in hair follicles and epidermis during embryonic development. They account for about 10﹪ of basal cell. There are a small number of melanocytes in hair follicle, choroid membranes and vasa aurisinternae. The decrease of cell number or dysfunction of melanocytes will results in pigment disorders. Research about melanocytes in epidermis of skin is abundant and the culture technique is mature. However, no theory is widely accepted for hair color fading. Melanocytes in hair follicles can produce melanin to protect the skin from ultraviolet light radiation, and home to the skin post-transplant. These evidences provide theoretical and experimental basis for the treatment of pigment disorder. We review the current progress and future direction about the biological characteristics and cultivation technique of hair follicle-derived melanocytes.

Key words: Hair Follicle, Melanocytes, Biology, Cell culture techniques
图1 黑色素细胞的胚胎发育过程示意图[6]
图2 相差显微镜下观察到的隆突部位毛囊中毛囊源性黑色素细胞（×100）
图3 毛囊源性黑色素细胞的周期循环示意图[14]
图4 黑色素细胞分化迁移路径[17]
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