Molecular mechanism of neural differentiation in human embryonic stem cells by single-cell sequencing analysis

doi:10.3877/cma.j.issn.2095-1221.2021.01.001

Abstract

Abstract:

Objective

To explore the key target genes and molecular mechanisms of human embryonic stem cell differentiation into neural cells, and provide molecular theoretical basis for clinical targeted treatment of neurological rehabilitation patients.

Methods

Based on the chip data from GEO platform, the key Marker genes in the differentiation process of human embryonic stem cells were analyzed from the multi-molecular dimension (single cell differential genes, protein interaction networks, gene pathways, etc.) by using R language as well as using single cell sequencing method. Quality control and data filtering, PCA, TSNE analysis, cell trajectory analysis, GO enrichment analysis, KEGG enrichment analysis, KEGG pathway analysis were used to show the mechanism of Marker gene regulating human embryonic stem cell differentiation.

Results

GO functional enrichment analysis showed that Marker genes played a significant role in germ layer differentiation, extracellular matrix and signal transduction. Marker gene interaction network and KEGG pathway showed that characteristic Fibronectin 1 (FN1) , Nanog homeobox (NANOG) and Growth factor receptor-bound protein 2 (GRB2) genes were the key genes. KEGG pathway analysis showed that FN1 played a significant role in regulating extracellular matrix pathway, and NANOG and GRB2 played significant roles in regulating stem cell pluripotency signaling pathway.

Conclusion

FN1 may mediate the matrix environment of human embryonic stem cells by regulating extracellular matrix pathways and up-regulation of NANOG and GRB2 in stem cell pluripotency signaling pathway could mediate directional differentiation of human embryonic stem cells into neural tissue.

Key words: Human embryonic stem cell, Neural progenitor cell, Single cell sequencing, Target genes, Molecular mechanism

Bing Cao, Xiaoming Zhang, Fulong Liang. Molecular mechanism of neural differentiation in human embryonic stem cells by single-cell sequencing analysis[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2021, 11(01): 1-7.

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Figures/Tables 9

References 26

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