Advances in research of islet organoids

doi:10.3877/cma.j.issn.2095-1221.2021.02.008

Abstract

Abstract:

Organoids are defined as three-dimensional cellular aggregations differentiated from pluripotent stem cells or adult tissues by cultivating in specific environment, which recapitulate the intricate pattern and function of the original tissue. Organoids play an important role in disease modeling and drug screening. In recent years, differentiating pancreatic tissue or stem cells into pancreatic islet organoids have become a hotspot and provided new ideas for the treatment of diabetes. In this paper, the vitro induction methods and the application prospect of islet organoids are reviewed.

Key words: Organoids, Islet, Human pluripotent stem cells

Qian Wu, Mei Zhang. Advances in research of islet organoids[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2021, 11(02): 120-124.

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URL: https://zhxbygxbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-1221.2021.02.008

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References 31

1	DiMeglio LA, Evans-Molina C, Oram RA. Type 1 diabetes[J]. Lancet, 2018, 391(10138):2449-2462.
2	Bretzel RG, Eckhard M, Brendel MD. Pancreatic islet and stem cell transplantation: new strategies in cell therapy of diabetes mellitus[J]. Panminerva Med, 2004, 46(1):25-42.
3	Dadheech N, James Shapiro AM. Human induced pluripotent stem cells in the curative treatment of diabetes and potential impediments ahead[J]. Adv Exp Med Biol, 2019, 1144:25-35.
4	Wu Q, Zheng S, Qin Y, et al. Efficacy and safety of stem cells transplantation in patients with type 1 diabetes mellitus-a systematic review and meta-analysis[J]. Endocr J, 2020, 67(8):827-840.
5	Lancaster MA, Knoblich JA. Organogenesis in a dish: modeling development and disease using organoid technologies[J]. Science, 2014, 345(6194):1247125.
6	Sato T, Vries RG, Snippert HJ, et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche[J]. Nature, 2009, 459(7244):262-265.
7	McCracken KW, Aihara E, Martin B, et al. Wnt/beta-catenin promotes gastric fundus specification in mice and humans[J]. Nature, 2017, 541(7636):182-187.
8	Uchida H, Machida M, Miura T, et al. A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells[J]. JCI insight, 2017, 2(1):e86492.
9	Foster J W, Wahlin K, Adams SM, et al. Cornea organoids from human induced pluripotent stem cells[J]. Sci Rep, 2017, 7:41286.
10	Chen YW, Huang SX, de Carvalho A, et al. A three-dimensional model of human lung development and disease from pluripotent stem cells[J]. Nat Cell Biol, 2017, 19(5):542-549.
11	Crespo M, Vilar E, Tsai SY, et al. Colonic organoids derived from human induced pluripotent stem cells for modeling colorectal cancer and drug testing[J]. Nat Med, 2017, 23(7):878-884.
12	Kim Y, Kim H, Ko UH, et al. Islet-like organoids derived from human pluripotent stem cells efficiently function in the glucose responsiveness in vitro and in vivo[J]. Sci Rep, 2016, 6:35145.
13	Mandarim-de-Lacerda CA. Pancreatic islet (of Langerhans) revisited[J]. Histol Histopathol, 2019, 34(9):985-993.
14	Benitez CM, Goodyer WR, Kim SK. Deconstructing pancreas developmental biology[J]. Cold Spring Harb Perspect Biol, 2012, 4(6):a012401.
15	Takahashi Y, Takebe T, Taniguchi H. Methods for generating vascularized islet-like organoids via self-condensation[J]. Curr Protoc Stem Cell Biol, 2018, 45(1):e49.
16	Loomans CJM, Williams Giuliani N, Balak J, et al. Expansion of adult human pancreatic tissue yields organoids harboring progenitor cells with endocrine differentiation potential[J]. Stem Cell Reports, 2018, 10(3):712-724.
17	Jiang K, Chaimov D, Patel SN, et al. 3-D physiomimetic extracellular matrix hydrogels provide a supportive microenvironment for rodent and human islet culture[J]. Biomaterials, 2019, 198:37-48.
18	Lebreton F, Lavallard V, Bellofatto K, et al. Insulin-producing organoids engineered from islet and amniotic epithelial cells to treat diabetes[J]. Nat Commun, 2019, 10(1):4491.
19	Wang D, Wang J, Bai L, et al. Long-term expansion of pancreatic islet organoids from resident procr(+) progenitors[J]. Cell, 2020, 180(6):1198-1211.e19.
20	Elizondo DM, Brandy NZD, da Silva RLL, et al. Pancreatic islets seeded in a novel bioscaffold forms an organoid to rescue insulin production and reverse hyperglycemia in models of type 1 diabetes[J]. Sci Rep, 2020, 10(1):4362.
21	D'Amour KA, Bang AG, Eliazer S, et al. Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells[J]. Nat Biotechnol, 2006, 24(11):1392-1401.
22	Zhang D, Jiang W, Liu M, et al. Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells[J]. Cell Res, 2009, 19(4):429-438.
23	Shim JH, Kim J, Han J, et al. Pancreatic islet-like three-dimensional aggregates derived from human embryonic stem cells ameliorate hyperglycemia in streptozotocin-induced diabetic mice[J]. Cell Transplant, 2015, 24(10):2155-2168.
24	Wang W, Jin S, Ye K. Development of islet organoids from H9 human embryonic stem cells in biomimetic 3D scaffolds[J]. Stem Cells Dev, 2017, 26(6):394-404.
25	Candiello J, Grandhi TSP, Goh SK, et al. 3D heterogeneous islet organoid generation from human embryonic stem cells using a novel engineered hydrogel platform[J]. Biomaterials, 2018, 177:27-39.
26	Bi H, Ye K, Jin S. Proteomic analysis of decellularized pancreatic matrix identifies collagen V as a critical regulator for islet organogenesis from human pluripotent stem cells[J]. Biomaterials, 2020, 233:119673.
27	Yoshihara E, O’Connor C, Gasser E, et al. Immune-evasive human islet-like organoids ameliorate diabetes[J]. Nature, 2020, 586(7830):606-611.
28	Wang H, Liu H, Zhang X, et al. One-step generation of aqueous-droplet-filled hydrogel fibers as organoid carriers using an all-in-water microfluidic system[J]. ACS Appl Mater Interfaces, 2021, 13(2):3199-3208.
29	Skardal A, Shupe T, Atala A. Organoid-on-a-chip and body-on-a-chip systems for drug screening and disease modeling[J]. Drug Discov Today, 2016, 21(9):1399-1411.
30	Tao T, Wang Y, Chen W, et al. Engineering human islet organoids from iPSCs using an organ-on-chip platform[J]. Lab Chip, 2019, 19(6):948-958.
31	Lebreton F, Wassmer CH, Belofatto K, et al. [Insulin-secreting organoids: a first step towards the bioartificial pancreas][J]. Med Sci (Paris), 2020, 36(10):879-885.

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