切换至 "中华医学电子期刊资源库"
高级检索
中华细胞与干细胞杂志(电子版)

中华细胞与干细胞杂志(电子版) ›› 2018, Vol. 08 ›› Issue (04) : 229 -233. doi: 10.3877/cma.j.issn.2095-1221.2018.04.007

所属专题： 文献；

综述

人脐带间充质干细胞移植治疗脊髓损伤的研究进展
陈奇1, 孙翊夫1, 吕晓霞2, 张福强3, 顾锐1, 高旭1, 邵立伟1, 姜金兰4,†()   
  1. 1. 130033　长春，吉林大学中日联谊医院骨科
    2. 250021　济南，山东附属省立医院中心实验室
    3. 130033　长春，吉林大学中日联谊医院科学研究中心
    4. 130033　长春，吉林大学中日联谊医院骨科；130033　长春，吉林大学中日联谊医院科学研究中心
  • 收稿日期:2018-04-10 出版日期:2018-08-01
  • 通信作者: 姜金兰

Progress of human umbilical cord mesenchymal stem cell transplantation for spinal cord injury

Qi Chen1, Yifu Sun1, Xiaoxia Lyu2, Fuqiang Zhang3, Rui Gu1, Xu Gao1, Liwei Shao1, Jinlan Jiang4,†()   

  1. 1. Department of Orthopedic Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China
    2. Central Laboratory of Shandong Provincial Hospital, Jinan 250021, China
    3. Scientific Research Center, China-Japan Union Hospital, Jilin University, Changchun 130033, China
    4. Department of Orthopedic Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China; Scientific Research Center, China-Japan Union Hospital, Jilin University, Changchun 130033, China
  • Received:2018-04-10 Published:2018-08-01
  • Corresponding author: Jinlan Jiang
  • About author:
    Corresponding author:Jiang Jinlan, Email:
全文 ( ) 下载PDF ( ) 导出引用
引用本文：

陈奇, 孙翊夫, 吕晓霞, 张福强, 顾锐, 高旭, 邵立伟, 姜金兰. 人脐带间充质干细胞移植治疗脊髓损伤的研究进展[J]. 中华细胞与干细胞杂志(电子版), 2018, 08(04): 229-233.

Qi Chen, Yifu Sun, Xiaoxia Lyu, Fuqiang Zhang, Rui Gu, Xu Gao, Liwei Shao, Jinlan Jiang. Progress of human umbilical cord mesenchymal stem cell transplantation for spinal cord injury[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2018, 08(04): 229-233.

脊髓损伤（SCI）由于复杂病理生理和神经修复再生困难，至今仍旧是难以攻克的医学难题，而干细胞因其神经再生和神经保护特性被认为是治疗SCI最有希望的方法。其中人脐带间充质干细胞（HUC-MSCs）近年培养分化方法不断改进、神经修复机制初步阐明，联合移植等综合治疗方案也不断实践，使HUC-MSCs移植治疗效果提高。另外关于HUC-MSCs治疗SCI的临床试验逐渐开展，术后患者神经功能恢复改善且无严重并发症出现，表明干细胞移植应用于人体是安全有效的。本文就HUC-MSCs治疗SCI的研究状况及进展进行综述。

关键词: 脊髓损伤, 人脐带间充质干细胞, 细胞移植, 联合治疗

Spinal cord injury (SCI) is still a difficult problem because of its complex pathophysiology. Stem cell transplantation are considered as the most promising treatment for SCI due to their nerve regeneration and neuroprotective effect. The methods of human umbilical cord mesenchymal stem cell (HUC-MSC) culture and differentiation have been improved. Moreover, the mechanism of neural repair has been preliminarily clarified. Combination therapy and other comprehensive treatment have improved the effect of stem cell transplantation. In addition, several clinical trials on stem cell therapy are on the way. HUC-MSCs transplantation can significantly improve the neurological function of patients without serious complications, indicating that stem cell therapy is safe and effective in human. This review summarizes and discusses recent advances on HUC-MSCs therapy for SCI.

Key words: Spinal cord injury, Human umbilical cord mesenchymal stem cells, Cell transplantation, Combination therapy
1
Sofroniew MV. Dissecting spinal cord regeneration[J]. Nature, 2018, 557(7705):343-350.
2
Ahuja CS,Nori S,Tetreault L, et al. Traumatic spinal cord injury-repair and regeneration[J]. Neurosurgery, 2017, 80(3S):S9-S22.
3
Gazdic M,Volarevic V,Harrell CR, et al. Stem cells therapy for spinal cord injury[J]. Int J Mol Sci, 2018, 19(4):E1039.
4
Li T,Xia M,Gao Y, et al. Human umbilical cord mesenchymal stem cells: an overview of their potential in cell-based therapy[J]. Expert Opin Biol Ther, 2015, 15(9):1293-1306.
5
Corrao S,La Rocca G,Lo Iacono M, et al. Umbilical cord revisited: from Wharton's jelly myofibroblasts to mesenchymal stem cells[J]. Histol Histopathol, 2013, 28(10):1235-1244.
6
Nan C,Shi Y,Zhao Z, et al. Monosialoteterahexosyl ganglioside induces the differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells[J]. Int J Mol Med, 2015, 36(4):1057-1062.
7
Li CS,Zheng Z,Su XX, et al. Activation of the extracellular signal-regulated kinase signaling is critical for human umbilical cord mesenchymal stem cell osteogenic differentiation[J]. Biomed Res Int, 2016, 2016:3764372.
8
De La Fuente A,Mateos J,Lesende-Rodriguez I, et al. Proteome analysis during chondrocyte differentiation in a new chondrogenesis model using human umbilical cord stroma mesenchymal stem cells[J]. Mol Cell Proteomics, 2012, 11(2):M111.010496.
9
Saben J,Thakali KM,Lindsey FE, et al. Distinct adipogenic differentiation phenotypes of human umbilical cord mesenchymal cells dependent on adipogenic conditions[J]. Exp Biol Med (Maywood), 2014, 239(10):1340-1351.
10
Jiang L,Wang Y,Pan F, et al. Synergistic effect of bioactive lipid and condition medium on cardiac differentiation of human mesenchymal stem cells from different tissues[J]. Cell Biochem Funct, 2016, 34(3):163-172.
11
Bharti D,Shivakumar SB,Park JK, et al. Comparative analysis of human Wharton's jelly mesenchymal stem cells derived from different parts of the same umbilical cord[J]. Cell Tissue Res, 2018, 372(1):51-65.
12
Wang H,Qiu X,Ni P, et al. Immunological characteristics of human umbilical cord mesenchymal stem cells and the therapeutic effects of their transplantion on hyperglycemia in diabetic rats[J]. Int J Mol Med, 2014, 33(2):263-270.
13
Batsali AK,Kastrinaki MC,Papadaki HA, et al. Mesenchymal stem cells derived from wharton's jelly of the umbilical cord:biological properties and emerging clinical applications[J]. Curr Stem Cell Res Ther, 2013, 8(2):144-155.
14
Li Y,Guo G,Li L, et al. Three-dimensional spheroid culture of human umbilical cord mesenchymal stem cells promotes cell yield and stemness maintenance[J]. Cell Tissue Res, 2015, 360(2):297-307.
15
Salehinejad P,Alitheen NB,Ali AM, et al. Neural differentiation of human umbilical cord matrix-derived mesenchymal cells under special culture conditions[J]. Cytotechnology, 2015, 67(3):449-460.
16
Dehghani-Soltani S,Shojaee M,Jalalkamali M, et al. Effects of light emitting diode irradiation on neural differentiation of human umbilical cord-derived mesenchymal cells[J]. Sci Rep, 2017, 7(1):9976.
17
Yang CC,Shih YH,Ko MH, et al. Transplantation of human umbilical mesenchymal stem cells from wharton's jelly after complete transection of the rat spinal cord[J]. PLoS One, 2008, 3(10):e3336.
18
Ryu HH,Kang BJ,Park SS, et al. Comparison of mesenchymal stem cells derived from fat,bone marrow,Wharton's jelly,and umbilical cord blood for treating spinal cord injuries in dogs[J]. J Vet Med Sci, 2012, 74(12):1617-1630.
19
Chen H,Zhang Y,Yang Z, et al. Human umbilical cord Wharton's jelly-derived oligodendrocyte precursor-like cells for axon and myelin sheath regeneration[J]. Neural Regen Res, 2013, 8(10):890-899.
20
Li C,Chen X,Qiao S, et al. Effects of wharton's jelly cells of the human umbilical cord on acute spinal cord injury in rats, and expression of interleukin-1 and nerve growth factor in spinal cord tissues[J]. Artif Cells Nanomed Biotechnol, 2016, 44(5):1254-1258.
21
Yang CH,Yu BQ,You QH, et al. Human umbilical cord mesenchymal stem cell transplantation for the treatment of two noncontinuous segments spinal cord compression injury in rabbits[J]. Zhonghua Yi Xue Za Zhi, 2017, 97(30):2366-2371.
22
Krupa P,Vackova I,Ruzicka J, et al. The effect of human mesenchymal stem cells derived from Wharton's jelly in spinal cord injury treatment is dose-dependent and can be facilitated by repeated application[J].Int J Mol Sci, 2018, 19(5):E1503.
23
Dalamagkas K,Tsintou M,Seifalian AM, et al. Stem cells for spinal cord injuries bearing translational potential[J]. Neural Regen Res, 2018, 13(1):35-42.
24
张敬星. 基于干细胞移植的联合治疗策略治疗脊髓损伤的实验研究进展[J]. 国际神经病学神经外科学杂志, 2014, 41(1):62-65.
25
Wang X,Wang YY,Zhang LL, et al. Combinatory effect of mesenchymal stromal cells transplantation and quercetin after spinal cord injury in rat[J]. Eur Rev Med Pharmacol Sci, 2018, 22(9):2876-2887.
26
Yan-Wu G,Yi-Quan K,Ming L, et al. Human umbilical Cord-Derived Schwann-Like cell transplantation combined with neurotrophin-3 administration in dyskinesia of rats with spinal cord injury[J]. Neurochem Res, 2011, 36(5):783-792.
27
白刚,张洪钿,赖军, 等. NT-3-HUMSCs联合基因沉默SOCS3治疗SD大鼠脊髓损伤后的神经再生修复[J]. 昆明医科大学学报, 2018, 39(3):31-37.
28
Jiao G,Lou G,Mo Y, et al. A combination of GDNF and hUCMSC transplantation loaded on SF/AGs composite scaffolds for spinal cord injury repair[J]. Mater Sci Eng C Mater Biol Appl, 2017, 74:230-237.
29
陈洪丽,屠梦茹,侯振豪, 等. 弱激光联合间充质干细胞对大鼠脊髓损伤的修复作用研究[J]. 中国激光医学杂志, 2018, 27(2):71.
30
Zhang RP,Wang LJ,He S, et al. Effects of magnetically guided, SPIO-Labeled, and neurotrophin-3 Gene-Modified bone mesenchymal stem cells in a rat model of spinal cord injury[J]. Stem Cells Int, 2016, 2016:2018474.
31
Hu SL,Luo HS,Li JT, et al. Functional recovery in acute traumatic spinal cord injury after transplantation of human umbilical cord mesenchymal stem cells[J]. Crit Care Med, 2010, 38(11):2181-2189.
32
魏开斌,卓锋,刘红, 等. 静脉移植人脐带间充质干细胞治疗急性脊髓损伤[J]. 中华实验外科杂志, 2012, 29(12):2579.
33
Karamouzian S,Nematollahi-Mahani SN,Nakhaee N, et al. Clinical safety and primary efficacy of bone marrow mesenchymal cell transplantation in subacute spinal cord injured patients[J]. Clin Neurol Neurosurg, 2012, 114(7):935-939.
34
刘雨亮,李军,何玉芹, 等. 脐带间充质干细胞移植治疗脊髓损伤:不同方式的疗效对比[J]. 中国组织工程研究, 2015, 19(14):2192-2198.
35
韩明远,冯世庆,李辉, 等. 蛛网膜下腔移植人脐带间充质干细胞修复大鼠脊髓损伤[J]. 中华实验外科杂志, 2010, 27(11):1645-1647.
36
Libro R,Bramanti P,Mazzon E. The combined strategy of mesenchymal stem cells and tissue-engineered scaffolds for spinal cord injury regeneration[J]. Exp Ther Med, 2017, 14(4):3355-3368.
37
王颖,冯世庆,赵鹏, 等. 大鼠脊髓损伤后不同时期移植人脐带间充质干细胞的修复效果观察[J]. 中国脊柱脊髓杂志, 2010, 20(11):918-925.
38
陈胜,金正帅. 不同时期局部移植人脐带间充质干细胞修复脊髓损伤的组织学观察[J]. 中国组织工程研究, 2016, 20(45):6714-6719.
39
Cheng H,Liu X,Hua R, et al. Clinical observation of umbilical cord mesenchymal stem cell transplantation in treatment for sequelae of thoracolumbar spinal cord injury[J]. J Transl Med, 2014, 12:253.
40
张赞,代广辉,刘学彬, 等. 脐带间充质干细胞移植治疗脊髓损伤疗效观察[J]. 中华实用诊断与治疗杂志, 2015, 29(5):478-480.
41
刘静,韩冬梅,薛梅, 等. 脐带间充质干细胞鞘内注射治疗脊髓损伤的长期临床观察[J]. 组织工程与重建外科杂志, 2017, 13(6):313-317.
42
Zhao Y,Tang F,Xiao Z, et al. Clinical study of NeuroRegen scaffold combined with human mesenchymal stem cells for the repair of chronic complete spinal cord injury[J]. Cell Transplant, 2017, 26(5):891-900.
43
Xiao Z,Tang F,Zhao Y, et al. Significant improvement of acute complete spinal cord injury patients diagnosed by a combined criteria implanted with NeuroRegen scaffolds and mesenchymal stem cells[J]. Cell Transplant, 2018, 27(6):907-915.
44
李脉. 干细胞移植治疗脊髓损伤的临床研究[D]. 昆明:昆明医科大学, 2012.
45
侯会荣,陆红岩,郑利星. 干细胞移植治疗脊髓损伤18例[J]. 脑与神经疾病杂志, 2012, 20(3):173-176.
46
张效北,李江涛,赵和泰, 等. 间质干细胞治疗脊髓损伤的临床分析 [J]. 亚太传统医药, 2012, 8(3):116-117.
47
Dai G,Liu X,Zhang Z, et al. Comparative analysis of curative effect of CT-guided stem cell transplantation and open surgical transplantation for sequelae of spinal cord injury[J]. J Transl Med, 2013, 11:315.
48
王培申,刘学彬,伊龙, 等. 脐带间充质干细胞鞘内移植治疗不完全性颈髓损伤的疗效和安全性[J]. 武警医学, 2015, 26(3):282-285.
49
饶耀剑,朱文潇,贾春霞, 等. 脐带间充质干细胞移植治疗脊髓损伤的临床观察[J]. 中国医药指南, 2013 (11):189-190.
50
刘静,韩冬梅,王志东, 等. 脐带间充质干细胞治疗脊髓损伤临床分析[J]. 中华损伤与修复杂志(电子版), 2011, 06(4):564-570.
51
Liu J,Han D,Wang Z, et al. Clinical analysis of the treatment of spinal cord injury with umbilical cord mesenchymal stem cells[J]. Cytotherapy, 2013, 15(2):185-191.
52
杨华强,张荣环,杜玲, 等. 脐带间充质干细胞移植治疗脊髓损伤的临床研究[J]. 现代生物医学进展, 2012, 12(3):518-521.
53
徐远红,王俊华,李海峰, 等. 脐带间充质干细胞移植联合综合康复训练对脊髓损伤患者神经功能及功能独立性的影响[J]. 中国康复医学杂志, 2015, 30(8):828-830.
[1] 陈帅, 刘文宾, 吴迪炯, 俞庆宏, 陈均法, 庄海峰, 胡致平, 武利强, 郑智茵, 沈建平, 叶宝东. 改良FAC预处理方案在不同供者类型行异基因造血干细胞移植治疗再生障碍性贫血中的疗效分析[J]. 中华危重症医学杂志(电子版), 2022, 15(04): 296-299.
[2] 曹叙勇, 刘耀升. 脊柱转移瘤手术并发症研究进展[J]. 中华损伤与修复杂志(电子版), 2022, 17(05): 435-439.
[3] 尚力凝, 杜成周, 郭刚, 陈德合, 王杰, 陈鹏, 李洪涛. 程序性细胞死亡受体1抑制剂治疗进展期胃癌的研究进展[J]. 中华普通外科学文献(电子版), 2022, 16(05): 376-381.
[4] 孔欣, 宋宝全, 刘吟, 张剑, 仇惠英, 吴德沛. 异基因造血干细胞移植并发难治性呃逆一例[J]. 中华移植杂志(电子版), 2023, 17(04): 253-255.
[5] 赵爽, 马梁明, 朱秋娟, 贡蓉, 高志林, 田卫伟, 王涛. 不同预处理方案对重型再生障碍性贫血患者单倍体造血干细胞移植的疗效分析[J]. 中华移植杂志(电子版), 2022, 16(04): 224-230.
[6] 陈敏, 潘田中, 孙自敏. 祛铁治疗异基因造血干细胞移植术后三系植入不良一例[J]. 中华移植杂志(电子版), 2022, 16(03): 168-171.
[7] 王楠楠, 刘文红, 孙立, 王婧. 脊髓损伤并发腹股沟嵌顿疝29例围手术期护理体会[J]. 中华疝和腹壁外科杂志(电子版), 2023, 17(04): 473-476.
[8] 万秋, 杨伏萍, 唐莉歆. 含氯法齐明的联合方案治疗耐多药肺结核的疗效及安全性分析[J]. 中华肺部疾病杂志(电子版), 2023, 16(02): 275-277.
[9] 刘文慧, 吴涛, 张曦. 间充质干细胞联合血小板生成素受体激动剂在异基因造血干细胞移植后血小板恢复中的研究进展[J]. 中华细胞与干细胞杂志(电子版), 2023, 13(04): 242-246.
[10] 李芸芸, 吴涛, 毛东锋, 鱼玲玲, 刘文慧. 轻型β-地中海贫血供者异基因造血干细胞移植治疗重型再生障碍性贫血1例[J]. 中华细胞与干细胞杂志(电子版), 2023, 13(02): 84-86.
[11] 苏春艳, 吴涛, 毛东锋, 刘文慧, 鱼玲玲, 白海. 异基因造血干细胞移植治疗急性混合细胞白血病后继发外周T细胞淋巴瘤1例[J]. 中华细胞与干细胞杂志(电子版), 2022, 12(05): 289-292.
[12] 姚尧, 杨新明, 杜雅坤, 朱宁, 阴彦林, 贾永利, 张瑛, 张培楠, 田野, 陈丽星. 雷公藤甲素与甲泼尼龙调节细胞自噬和凋亡促进脊髓损伤修复的比较研究[J]. 中华神经创伤外科电子杂志, 2022, 08(03): 132-140.
[13] 李俸鑫, 许建文, 陈如玉, 李常秋, 王继羚, 谭秀伟, 卜海峰, 王海霖, 苏义基. 2015至2020年广西医科大学第一附属医院老年脊髓损伤的特征分析[J]. 中华老年骨科与康复电子杂志, 2023, 09(01): 45-50.
[14] 左安俊, 欧振飞, 王天瑞, 丁磊, 李天予, 于腾波. 二甲胺四环素对小胶质细胞激活状态影响的研究[J]. 中华老年骨科与康复电子杂志, 2022, 08(03): 152-158.
[15] 赵洁, 王岚, 杨红枚, 何屹, 袁红. 异基因造血干细胞移植后并发自身免疫性溶血性贫血患者产生类抗体血清学检测方法及输血策略[J]. 中华临床医师杂志(电子版), 2022, 16(05): 452-456.
阅读次数
全文


摘要

版权所有 中华医学会 中华医学电子音像出版社有限责任公司  京ICP备14006079号-1  网络出版服务许可证：(署)网出证(京)字第075号