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中华细胞与干细胞杂志(电子版) ›› 2018, Vol. 08 ›› Issue (04) : 224 -228. doi: 10.3877/cma.j.issn.2095-1221.2018.04.006

所属专题: 文献

论著

影响脐带血采集质量的相关性因素分析
张晋1,(), 袁哲2   
  1. 1. 110001 沈阳,中国医科大学附属盛京医院检验科
    2. 110001 沈阳,中国医科大学附属盛京医院脐血库
  • 收稿日期:2018-04-11 出版日期:2018-08-01
  • 通信作者: 张晋

Analysis of factors associated with the quality of umbilical cord blood

Jin Zhang1,(), Zhe Yuan2   

  1. 1. Department of Laboratory Medicine, Shengjing Hospital of China Medical University, Shenyang 110001, China
    2. Cord Blood Bank of Liaoning Province, Shengjing Hospital of China Medical University, Shenyang 110034, China
  • Received:2018-04-11 Published:2018-08-01
  • Corresponding author: Jin Zhang
  • About author:
    Corresponding author:Zhang Jin, Email:
引用本文:

张晋, 袁哲. 影响脐带血采集质量的相关性因素分析[J/OL]. 中华细胞与干细胞杂志(电子版), 2018, 08(04): 224-228.

Jin Zhang, Zhe Yuan. Analysis of factors associated with the quality of umbilical cord blood[J/OL]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2018, 08(04): 224-228.

目的

回顾性分析影响脐带血采集质量的母婴因素和采集处理因素。

方法

记录389份脐带血的采集量、母亲年龄、孕龄、新生儿体重、分娩方式、新生儿性别、胎次及脐带血采集至计数间隔以及采集方式。用KX-21型全自动血液分析仪进行细胞计数并计算有核细胞总数(TNC)。采用Pearson相关和Spearman秩相关进行相关性分析,采用t检验、Mann-Whitney U检验、方差分析及Kruskal-Wallis H检验进行分组比较,分析影响脐带血采集量和TNC的相关因素。?

结果

脐带血采集量与TNC显著相关(r = 0.723,P < 0.001),脐带血采集量大于80 ml时的TNC显著高于低体积者。在母婴因素中,母亲年龄与采集量及TNC差异均无统计学意义;孕龄与采集量负相关(r = -0.119,P = 0.019),而与TNC正相关(r = 0.138,P = 0.007),足月儿脐带血的TNC显著高于早产儿(P = 0.038);婴儿出生体重与采集量及TNC均正相关(r = 0.236,P < 0.001;r = 0.275,P < 0.001),体重较大婴儿脐带血的采集量和TNC均显著高于体重较小者(P?< 0.001);剖宫产的脐带血采集量虽高于阴道分娩(P < 0.001),但其TNC不及阴道分娩;男婴与女婴在脐带血采集量和TNC之间无显著差异。在采集处理因素中,脐带血采集至计数的时间间隔与采集量及TNC均无显著相关。

结论

为提高脐带血的保存质量,应侧重选择胎儿体重较大、经阴道分娩的产妇作为脐带血供者,实验室应首先处理采集量较大的脐带血。

Objective

To evaluate the maternal, infant and collection-processing factors associated with the quality of umbilical cord blood(UCB).

Methods

389 umbilical cord blood units were recorded, including collection volume, mother's age, gestational age, infant birth weight, delivery way, sex of newborn, number of pregnancy, time interval between collection and counting and collection method. The total nucleated cells (TNC) were counted by KX-21 automatic blood analyzer. Pearson correlation and Spearman rank correlation were used for correlation analysis, t test, mann-whitney U test, variance analysis and kruskal-wallis H test were used for group comparison, and the correlation factors affecting umbilical cord blood collection volume and TNC were analyzed.

Results

There was a significant correlation between collection volume and TNC (r = 0.723, P?< 0.001). As for maternal and infant factors, mother's age had no correlation between collection volume and TNC. Gestational age correlated negatively with collection volume (r = -0.119, P = 0.019) and positively with TNC (r = 0.138, P = 0.007). The TNC from full-term infants was significantly higher than that of preterm ones (P = 0.038). The infant birth weight correlated strongly with collection volume (r = 0.236, P < 0.001) and TNC (r = 0.275, P < 0.001). Although the collection volume of cesarean section was higher than that of vaginal delivery (P < 0.001), its TNC was lower than that of vaginal delivery. There was no difference between male and female infants in collection volume and TNC. As for collection-processing factors, the time interval between collection and counting had no correlation between collection volume and TNC.

Conclusion

These results suggest that infant weight and vaginal delivery are associated with TNC.

表1 脐带血采集量及TNC与母婴及采集处理因素相关性分析
表2 母婴及采集处理因素对脐带血采集量及TNC的影响
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