Repairment of anchilles tendon injury by adipose derived stromal vascular fraction in rabbits

doi:10.3877/cma.j.issn.2095-1221.2021.04.006

Abstract

Abstract:

Objective

To investigate the effects of intratendinous injection of adipose-derived stromal vascular fraction (SVF) on the repair of rabbit Achilles tendon injury.

Methods

Rabbit Achilles tendon injury model was induced by prostaglandin E2 (PGE2) . The injury model group received PGE2 injections, while the non-injury control group was injected with equal doses of saline. Ultrasonography, HE staining and Masson staining were used to evaluate the modeling. And the model group were randomly divided into two groups. The SVF treatment group was injected with rabbit-derived adipose SVF locally in the Achilles tendon, and the model control group was injected with equal doses of saline, respectively. Then, at 3 and 8 weeks after treatment, ultrasonography examination was performed. Achilles tendon tissue samples were obtained and examined histologically. Expression of collagen I and III was determined immunohistochemically. One-way ANOVA was used between multiple groups, with further multiple comparisons between groups performed by LSD.

Results

Ultrasound results revealed that tendon tissue had localized swelling and heterogeneous internal echoes after the induction of PGE2. The results of HE and Masson staining revealed visible fiber fracture, vascular proliferation, inflammatory cell infiltration, and fatty degeneration. Compared with model control group, the hypoechogenicity and inflammatory cells were reduced, the structure of fibers was improved and inflammatory cells were reduced in SVF group was at 3 and 8 weeks after treatment, and the expression of collagen I (week 3: 0.2948 ± 0.0126 vs 0.3186 ± 0.0108, week 8: 0.3127 ± 0.0157 vs 0.3427 ± 0.0138) , collagen III (week 3: 0.2815 ± 0.0143 vs 0.3025 ± 0.0141, week 8: 0.2981 ± 0.0153 vs 0.3213 ± 0.0104) was all increased. All differences were considered statistically significant (all P < 0.05) .

Conclusions

SVF can reduce the inflammatory reaction of tendon, improve the structure and morphology of collagen fiber, up-regulate the expression level of collagen I and III, and promote the healing of Achilles tendon injury in rabbits.

Key words: Stromal vascular fraction, Adipose stem cells, Tendon, Prostaglandin E2, Achilles tendon injury

Shun Lin, Jingpeng Lin, Jin Chen, Wanming Wang, Xuesong Han. Repairment of anchilles tendon injury by adipose derived stromal vascular fraction in rabbits[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2021, 11(04): 231-239.

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References 41

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分组	动物数	Ⅰ型胶原		Ⅲ型胶原
分组	6	3周	8周	3周	8周
空白对照组	6	0.3948±0.0112^b	0.3934±0.0107^b	0.3590±0.0123^b	0.3572±0.0115^b
模型对照组	6	0.2948±0.0126^a	0.3127±0.0157^a	0.2815±0.0143^a	0.2981±0.0153^a
SVF治疗组	6	0.3186±0.0108^ab	0.3427±0.0138^ab	0.3025±0.0141^ab	0.3213±0.0104^ab
F值		102.406	43.746	45.085	28.000
P值		< 0.001	< 0.001	< 0.001	< 0.001

分组	动物数	Ⅰ型胶原		Ⅲ型胶原
分组	6	3周	8周	3周	8周
空白对照组	6	0.3948±0.0112^b	0.3934±0.0107^b	0.3590±0.0123^b	0.3572±0.0115^b
模型对照组	6	0.2948±0.0126^a	0.3127±0.0157^a	0.2815±0.0143^a	0.2981±0.0153^a
SVF治疗组	6	0.3186±0.0108^ab	0.3427±0.0138^ab	0.3025±0.0141^ab	0.3213±0.0104^ab
F值		102.406	43.746	45.085	28.000
P值		< 0.001	< 0.001	< 0.001	< 0.001

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