Adiponectin regulates proliferation, apoptosis, and insulin sensitivity through PERK/eIF2α-mediated endoplasmic reticulum stress in endometrial cancer cells

doi:10.3877/cma.j.issn.2095-1221.2021.04.007

Abstract

Abstract:

Objective

To explore whether adiponectin (ADPN) can regulate endoplasmic reticulum (ER) stress and cells proliferation, apoptosis or insulin sensitivity in endometrial cancer cells through PERK/eIF2α.

Methods

endometrial cancer cells (HEC-1A) were divided into control group (no treatment) , ADPN group and ADPN + Salubrinal (eIF2α dephosphorylation inhibitor) group. And the latter of two groups were treated with 20 μg/mL ADPN. And 10 μmol/L Salubrinal was added in the ADPN+Salubrinal group. The cell viability, proliferation and apoptosis were detected by CCK-8 method, clone formation experiment, and flow cytometry. PERK/eIF2α factors were detected by Western blot.

Results

The cell viability and cell proliferation ability (42.62±4.35 points) in the ADPN group were significantly lower than those of the control group (P < 0.001) . The cell viability and cell proliferation ability (127.63±5.28 points) in the ADPN+Salubrinal group were significantly higher than those of the ADPN group (P < 0.001) . The apoptotic rate of ADPN group at 48 h and 72 h [48 h: (7.38±0.20) ﹪, (5.57±0.18) ﹪, 72 h: (21.47±0.52) ﹪, (12.06±0.48) ﹪] were significantly higher than those of control group [48 h: (3.48±0.11) ﹪, 72 h: (7.25±0.24) ﹪] (P < 0.001) . Compared with the ADPN group, the apoptotic rate in the ADPN+Salubrinal group decreased, and the difference was statistically significant (P < 0.001) . The expression level of 2-NBDG uptake in ADPN group (1.85±0.13) was significantly higher than that in control group (1.00±0.06) (P < 0.001) . The expression level of 2-NBDG in ADPN+Salubrinal group (1.13±0.09, 1.08±0.09) was significantly lower than that in ADPN group (P < 0.001) . The protein levels of PERK and p-eIF2α/eIF2α in the ADPN group (1.72±0.14, 0.42±0.03) were significantly lower than those in the control group (P < 0.001) . The protein level of p-eIF2α/eIF2α in ADPN+Salubrinal group (0.94±0.09) was significantly higher than that in ADPN group (P < 0.001) .

Conclusion

ADPN may regulate cell proliferation, apoptosis and insulin sensitivity through PERK/eIF2α pathway in endometrial cancer cells.

Key words: Endometrial cancer, Adiponectin, Insulin sensitivity, Endoplasmic reticulum stress, PERK, eIF2α

Cuirong Zhang. Adiponectin regulates proliferation, apoptosis, and insulin sensitivity through PERK/eIF2α-mediated endoplasmic reticulum stress in endometrial cancer cells[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2021, 11(04): 240-245.

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References 21

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分组	实验次数	24 h	48 h	72 h
对照	3	0.38±0.03	0.65±0.06	1.12±0.09
ADPN	3	0.33±0.04	0.46±0.06^a	0.68±0.07^a
ADPN+Salubrinal	3	0.35±0.01	0.55±0.08^ab	0.92±0.08^ab
F值		2.192	5.978	22.515
P值		0.193	0.037	0.002

分组	实验次数	24 h	48 h	72 h
对照	3	0.38±0.03	0.65±0.06	1.12±0.09
ADPN	3	0.33±0.04	0.46±0.06^a	0.68±0.07^a
ADPN+Salubrinal	3	0.35±0.01	0.55±0.08^ab	0.92±0.08^ab
F值		2.192	5.978	22.515
P值		0.193	0.037	0.002

分组	实验次数	克隆形成数目（个）
对照	3	127.63±5.28
ADPN	3	42.62±4.35^a
ADPN+Salubrinal	3	73.05±5.86^ab
F值		205.785
P值		< 0.001

分组	实验次数	克隆形成数目（个）
对照	3	127.63±5.28
ADPN	3	42.62±4.35^a
ADPN+Salubrinal	3	73.05±5.86^ab
F值		205.785
P值		< 0.001

分组	实验次数	24 h	48 h	72 h
对照	3	2.14±0.01	3.48±0.11	7.25±0.24
ADPN	3	3.01±0.09	7.38±0.20^a	21.47±0.52^a
ADPN+Salubrinal	3	2.56±0.08	5.57±0.18^ab	12.06±0.48^ab
F值		116.692	405.696	843.193
P值		< 0.001	< 0.001	< 0.001

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