Progress in biology and in vitro culture of hair follicle-derived melanocytes

doi:10.3877/cma.j.issn.2095-1221.2017.02.010

Abstract

Abstract:

Melanocytes are main cells in the vertebrate for staining skin and fair and locate in hair follicles and epidermis during embryonic development. They account for about 10﹪ of basal cell. There are a small number of melanocytes in hair follicle, choroid membranes and vasa aurisinternae. The decrease of cell number or dysfunction of melanocytes will results in pigment disorders. Research about melanocytes in epidermis of skin is abundant and the culture technique is mature. However, no theory is widely accepted for hair color fading. Melanocytes in hair follicles can produce melanin to protect the skin from ultraviolet light radiation, and home to the skin post-transplant. These evidences provide theoretical and experimental basis for the treatment of pigment disorder. We review the current progress and future direction about the biological characteristics and cultivation technique of hair follicle-derived melanocytes.

Key words: Hair Follicle, Melanocytes, Biology, Cell culture techniques

Ruirui Fan, Feilin Liu, Deshun Hao, Qi Zhang, Yingyao Zhang, Tong Zheng, Shilun Li, Hongyan Wang, Liangchen Qi, Jinyu Liu. Progress in biology and in vitro culture of hair follicle-derived melanocytes[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2017, 07(02): 117-123.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhxbygxbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-1221.2017.02.010

https://zhxbygxbzz.cma-cmc.com.cn/EN/Y2017/V07/I02/117

Figures/Tables 4

References 48

1	姜俊兵，董常生，贺俊平. 不同被毛颜色羊驼皮肤组织中成熟黑色素细胞的组织学分析[J]. 畜牧兽医学报, 2010, 41(2):229-233.
2	Tu S, Johnson SL. Clonal analyses reveal roles of organ founding stem cells, melanocyte stem cells and melanoblasts in establishment, growth and regeneration of the adult zebrafish fin[J]. Development, 2010, 137(23):3931-3939.
3	Adameyko I, Lallemend F, Aquino JB, et al. Schwann cell precursors from nerve innervation are a cellular origin of melanocytes in skin[J]. Cell, 2009, 139(2):366-379.
4	Dupin E, Sommer L. Neural crest progenitors and stem cells: From early development to adulthood[J]. DevBiol, 2012, 366(1, SI):83-95.
5	Mort RL, Jackson IJ, Patton EE. The melanocyte lineage in development and disease[J]. Development, 2015, 142(4):620-632.
6	Botchkareva NV, Khlgatian M, Longley BJ, et al. SCF/c-kit signaling is required for cyclic regeneration of the hair pigmentation unit[J]. FASEB J, 2001, 15(3):645-658.
7	Nishimura EK, Suzuki M, Igras V, et al. Key roles for transforming growth factor beta in melanocyte stem cell maintenance[J]. Cell Stem Cell, 2010, 6(2):130-140.
8	Nishimura EK. Melanocyte stem cells: a melanocyte reservoir in hair follicles for hair and skin pigmentation[J]. Pigment Cell Melanoma Res, 2011, 24(3):401-410.
9	Wang J, Wang Y, Luo C, et al. Accumulation of melanin in the peritoneum causes black abdomens in broilers[J]. Poult Sci, 2014, 93(3):742-746.
10	Lo Cicero A, Delevoye C, Gilles-Marsens F, et al. Exosomes released by keratinocytes modulate melanocyte pigmentation[J]. Nat Commun, 2015, 6:7506.
11	Alonso L, Fuchs E. The hair cycle[J]. J Cell Sci, 2006, 119(Pt 3):391-393.
12	Barsh G, Cotsarelis G. How hair gets its pigmet[J]. Cell, 2007, 130(5):779-781.
13	邓芳，郭海英，连小华, 等. 毛囊黑素谱系细胞中β-catenin在毛囊周期中的表达[J]. 第三军医大学学报, 2011, 33(8):793-796.
14	Steingrimsson E, Copeland NG, Jenkins NA. Melanocyte stem cell maintenance and hair graying[J]. Cell, 2005, 121(1):9-12.
15	Aoki H, Hara A, Motohashi T, et al. Functionally distinct melanocyte populations revealed by reconstitution of hair follicles in mice[J]. Pigment Cell Melanoma Res, 2011, 24(1):125-135.
16	Fuchs E. Scrathing the surface of skin development[J].Nature, 2007, 445(7130):834-842.
17	Qiu WM, Yang K, Lei MX, et al. SCF/c-kit signaling is required in12-O-tetradecanoylphorbol-13-acetate-induced migration and differentiation of hair follicle melanocytes for epidermal pigmentation[J]. Cell TissueRes, 2015, 360(2):333-346.
18	Huelsken J, Vogel R, Erdmann B, et al. beta-Catenin controls hair follicle morphogenesis and stem cell differentiation in the skin[J]. Cell, 2001
19	Tobin DJ, Bystryn JC. Different populations of melanocytes are present in hair follicles and epidermis[J]. Pigment Cell Res, 1996, 9(6):304-310.
20	Van Neste D, Tobin DJ. Hair cycle and hair pigmentation: dynamic interactions and changes associated with aging[J]. Micron, 2004, 35(3):193-200.
21	Narisawa Y, Kohda H, Tanaka T. Three-dimensional demonstration of melanocyte distribution of human hair follicles: Special reference to the Bulge area[J]. ActaDermVenereol, 1997, 77(2):97-101.
22	谢光跃. 黑素细胞分布规律及基因差异表达[D]. 武汉: 华中农业大学, 2014 .
23	Schiaffino MV. Signaling pathways in melanosome biogenesis and pathology[J]. Int J Biochem Cell Biol, 2010, 42(7):1094-1104.
24	HurbainI, Geerts WJ, Boudier T, et al. Electron tomography of early melanosomes: implications for elanogenesis and the generation of fibrillar amyloid sheets[J]. ProcNatlAcadSci U S A, 2008, 105(50):19726-19731.
25	Slominski A, Tobin DJ, Shibahara S, et al. Melanin pigmentation in mammalian skin and its hormonal regulation[J]. Physiol Rev, 2004, 84(4):1155-1228.
26	Guo HY, Yang K, Deng F, et al. Wnt3a promotes melanin synthesis of mouse hair follicle melanocytes[J]. Biochem Biophys Res Commun, 2012, 420(4):799-804.
27	Chang H, Choi H, Joo KM, et al. Manassantin B inhibits melanosome transport in melanocytes by disrupting the melanophilin-myosin Va interaction[J]. Pigment Cell Melanoma Res, 2012, 25(6):765-772.
28	Thomas AJ, Erickson CA. The making of a melanocyte: the specification of melanoblasts from the neural crest[J]. Pigment Cell Melanoma Res, 2008, 21(6):598-610.
29	Matsui T, Ohbayashi N, Fukuda M. The Rab interacting ly-sosomalprotein(RILP)homology domain functions as a novel effector domain for small GTPase Rab36:Rab36 regulates retrograde melanosome transport in melanocytes[J]. J BiolChem, 2012, 287(34):28619-28631.
30	Wu XS, Masedunskas A, Weigert RA, et al. Melanoregulin regulates a shedding mechanism that drives melanosome transfer from melanocytes to keratinocytes[J]. ProcNatlAcadSci U S A, 2012, 109(31):E2101-E2109.
31	Ando H, Kondoh H, Ichihashi M, et al. Approaches to identify inhibitors of melanin biosynthesis via the quality control of tyrosinase[J]. Journal of investigative dermatology, 2007, 127(4):751-761.
32	Dooley CM, Mongera A, Walderich B, et al. On the embryonic origin of adult melanophores: the role of ErbB and Kit signalling in establishing melanophore stem cells in zebrafish[J]. Development, 2013, 140(5):1003-1013.
33	Hoogduijn MJ, Cemeli E, Ross K, et al. Melanin protects melanocytes and keratinocytes against H2O2-induced DNA Strand breaks through its ability to bind Ca2⁺[J]. Exp Cell Res, 2004, 294(1):60-67.
34	White RM, Zon Li. Melanocytes in development, regeneration, and cancer[J]. Cell Stem Cell, 2008, 3(3):242-252.
35	Wong G, Gupta R, Dixon KM, et al. 1,25 Dihydroxyvitamin D and three low-calcemic analogs decrease UV-induced DNA damage via the rapid response pathway[J]. J Steroid Biochem Mol Biol, 2004, 89-90(1-5):567-570.
36	Pavel S, Van Nieuwpoort F, Van Der Meulen H, et al. Disturbed melanin synthesis and chronic oxidative stress in dysplastic naevi[J].Eur J Cancer, 2004, 40(9):1423-1430.
37	Choi SY, Kim S, Hwang JS, et al. Benzylamide derivative compound attenuates the ultraviolet B-induced hyperpigmentation in the brownish Guinea pig skin[J]. Biochem Pharmacol, 2004, 67(4):707-715.
38	Lin SJ, Jee SH, Hsaio WC, et al. Formation of melanocyte spheroids on the chitosan-coated surface[J]. Biomaterials, 2005, 26(12):1413-1422.
39	耿建军，穆晓丽，孙乐天, 等. 内皮素受体B在不同毛色羊驼皮肤中的表达与定位[J]. 畜牧兽医学报, 2010, 41(11):1478-1484.
40	Dorshorst B, Molin AM, Rubin CJ, et al. A complex genomic rearrangement involving the endothelin 3 locus causes dermal hyperpigmentation in the chicken[J]. PLoS Genet, 2011, 7(12):e1002412.
41	Saldana-Caboverde A, Kos L. Roles of endothelin signaling in melanocyte development and melanoma[J]. Pigment Cell Melanoma Res, 2010, 23(2):160-170.
42	王大光，朱文元，马慧军, 等. 人毛囊无黑素黑色素细胞培养的纯化和培养基成分对细胞影响的实验研究[J]. 临床皮肤科杂志, 2004, 33(2):80-83.
43	Tang J, Li Q, Cheng B, et al. Effects of diethylstilbestrol on the proliferation and tyrosinase activity of cultured human melanocytes[J]. Biomed Rep, 2015, 3(4):499-502.
44	Motokawa T, Miwa T, Mochizuki M, et al. Adrenomedullin: a novel melanocyte dendrite branching factor[J]. J DermatolSci, 2015, 79(3):307-310.
45	Hwang I, Park JH, Park HS, et al. Neural stem cells inhibit melanin production by activation of Wnt inhibitors[J]. J Dermatol Sci, 2013, 72(3):274-283.
46	白瑞，于志慧，范瑞文, 等. 羊驼皮肤黑素细胞体外培养鉴定[J]. 畜牧兽医学报, 2014, 44(4):549-556.
47	Hu DN, Mccormick SA, Seedor JA, et al. Isolation, purification and cultivation of conjunctival melanocytes[J]. Exp Eye Res, 2007, 84(4):655-662.
48	屈颖，齐浩. 黑素细胞的分离与培养综述[J]. 西安文理学院学报(自然科学版), 2005, 8(4):12-15.

[1]	Jin Zhang, Jin Zheng, Chenxiao Ye, Haitao Chen, Xinrong Li, Haijuan Xiao, Yong Guo. Comprehensive analysis of prognosis and immune function of breast cancer patients based on glycolysis related gene model [J]. Chinese Journal of Breast Disease(Electronic Edition), 2022, 16(06): 336-345.
[2]	Yu Chen, Fang Feng, Lu Zhang, Jian Liu. Bioinformatics analysis to screen key pathogenic genes in septic cardiomyopathy [J]. Chinese Journal of Critical Care Medicine(Electronic Edition), 2023, 16(04): 286-291.
[3]	Zhehao Liang, Mingsun Fang, Hongyi Hu, Tao Tao, Xiaoping Xu, Huaqin Sun. Bioinformatics analysis to screen key genes in septic-induced acute lung injury [J]. Chinese Journal of Critical Care Medicine(Electronic Edition), 2022, 15(05): 360-366.
[4]	Fang Feng, Huyong Yang, Weiwei Yang, Yu Chen. Bioinformatics analysis to screen key pathogenic genes in acute exacerbation of chronic obstructive pulmonary disease [J]. Chinese Journal of Critical Care Medicine(Electronic Edition), 2022, 15(04): 265-270.
[5]	Huizhu Chen, Yingkun Guo, Xinrong Wang, Gang Ning, Xijian Chen. Current research status on the " dualistic model" of epithelial ovarian cancer and its molecular biology [J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(04): 394-402.
[6]	Yunwei Wang, Liang Luo, Peng Cao, Qingyi Zhang, Shaohui Li, Yang Chen, Hao Guan. Research progress of isolation and culture of dermal papilla cells [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2022, 17(06): 520-523.
[7]	Guojun Tang, Yude Hong, Chongyu Zhao, Liaoyuan Li. A novel prognosis model for papillary renal cell carcinoma based on Wnt-associated lncRNAs in TCGA database [J]. Chinese Journal of Endourology(Electronic Edition), 2023, 17(03): 270-275.
[8]	Jiuli Yuan, Dan Liu, Linli Li, Jinyu Liu. Basic research and clinical application of hair follicle-derived mesenchymal stem cells [J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2023, 13(03): 189-192.
[9]	Yingying Yang, Bo Diao, Yueping Liu, Qian Yang, Chuancheng Zhang, Gang Wang. The role of stem cells in hair regeneration [J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2022, 12(02): 110-114.
[10]	Jiantao Mo, Peize Yang, Ruiqi Cao, Qingyong Ma, Zheng Wang, Zheng Wu, Cancan Zhou. Establishment of a ferroptosis-related lncRNA prognostic model for patients with intrahepatic cholangiocarcinoma based on bioinformatics analysis [J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2023, 12(02): 185-189.
[11]	Fuping Li, Mao Wang, Rui Cheng, Shangpeng Li, Yinhai Dai. Bioinformatics analysis of the expression level and clinical significance of prefoldin family in hepatocellular carcinoma [J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2023, 12(02): 227-235.
[12]	Jiezhong Wu, Chunlian Ke, Shaohong Chen, Bo Liu, Zhicheng Yao. Mechanism of circZKSCAN1 inhibiting invasion and metastasis of hepatocellular carcinoma cells [J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2022, 11(01): 92-97.
[13]	Aiqun Li, Mingyue Mo, Yan Lin, Ke Wu, Zena Huang. Exploration and practice of ideological and political education in student-centered clinical microbiology laboratory testing techniques course [J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2023, 11(04): 249-255.
[14]	Aiwu Wu, Xiaoxia Kou, Wenchang Yuan, Yueting Jiang. Exploration and practise of the ideological and political education in laboratory medicine specialized courses—Taking the clinical microbiology course as an example [J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2023, 11(04): 245-248.
[15]	Bozong Shao. Teaching and training of experimental methods in molecular biology in digestive science research [J]. Chinese Journal of Gastrointestinal Endoscopy(Electronic Edition), 2023, 10(01): 67-69.

Please choose a citation manager

Content to export