Advances in research of induced differentiation of mesenchymal stem cells into alveolar epithelial cells for treatment of ARDS

doi:10.3877/cma.j.issn.2095-1221.2017.02.011

Abstract

Abstract:

Acute respiratory distress syndrome (ARDS) , which is notorious for its high mortality rate, is one of the greatest challenges in the field of respiratory and critical care medicine. Mesenchymal stem cell (MSC) , which is readily available and easily expandablein vitro, is considered to be a promising therapy for ARDS. Numerous studies have shown the effectiveness of MSC in the treatment of ARDS. Although the mechanism is not completely understood, differentiation of MSC into alveolar epithelial cells to repair injured lung tissue may be one of the possible mechanisms. Herein, research progress of induced differentiation of MSC into alveolar epithelial cells and its application in the treatment of ARDS is reviewed.

Key words: Mesenchymal stem cells, Pulmonary alveoli, Epithelial cells, Acute disease, Respiratory distress syndrome

Qingui Chen, Mian Zeng. Advances in research of induced differentiation of mesenchymal stem cells into alveolar epithelial cells for treatment of ARDS[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2017, 07(02): 124-128.

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References 38

1	Bellani G, Laffey JG, Pham T, et al. Epidemiology, patterns of care, and mortality for patients with acute respiratory distress syndrome in intensive care units in 50 countries[J]. JAMA, 2016, 315(8):788-800.
2	耿申，吴婷，穆先敏,等.细胞间黏附分子-1在ARDS小鼠肺微血管内皮细胞内的表达变化[J].医学研究生学报, 2016(04):342-347.
3	Han S, Mallampalli RK. The acute respiratory distress syndrome: from mechanism to translation[J].J Immunol, 2015, 194(3):855-860.
4	王凡，韩志海.炎症介质在海水淹溺急性肺损伤发生机制中的作用[J].转化医学杂志, 2015(05):311-315.
5	Horie S, Masterson C, Devaney J, et al. Stem cell therapy for acute respiratory distress syndrome: a promising future?[J]. Curr Opin Crit Care, 2016, 22(1):14-20.
6	Sweeney RM, McAuley DF. Acute respiratory distress syndrome[J]. Lancet, 2016, 388(10058):2416-2430.
7	Walter J, Ware LB, Matthay MA. Mesenchymal stem cells: mechanisms of potential therapeutic benefit in ARDS and sepsis[J]. Lancet Respir Med, 2014, 2(12):1016-1026.
8	Albera C, Polak JM, Janes S, et al. Repopulation of human pulmonary epithelium by bone marrow cells: a potential means to promote repair[J]. Tissue Eng, 2005, 11(7-8):1115-1121.
9	Kotton DN, Fabian AJ, Mulligan RC. Failure of bone marrow to reconstitute lung epithelium[J]. Am J Respir Cell Mol Biol, 2005, 33(4):328-334.
10	赵峰，李圣青，张宇飞,等.骨髓间充质干细胞在肺损伤大鼠肺组织的分化[J].解放军医学杂志, 2007, 32(2):131-133.
11	Wang W, Qian LL, Liu HP, et al. Engraftment of bone marrow stromal cells in lipopolysaccharide-injured mouse lungs[J]. Zhongguo Dang Dai Er Ke Za Zhi, 2009, 11(5):321-327.
12	Li JD. Directed differentiation of airway epithelial cells of human bone marrow mesenchymal stem cells[J]. Artif Cells Nanomed Biotechnol, 2016, 44(7):1654-1658.
13	Yan X, Liu Y, Han Q, et al. Injured microenvironment directly guides the differentiation of engrafted Flk-1(+) mesenchymal stem cell in lung[J]. Exp Hematol, 2007, 35(9):1466-1475.
14	Li Y, Xu W, Yan J, et al. Differentiation of human amniotic fluid-derived mesenchymal stem cells into type II alveolar epithelial cells in vitro[J]. Int J Mol Med, 2014, 33(6):1507-1513.
15	Maria OM, Maria AM, Ybarra N, et al. Mesenchymal stem cells adopt lung cell phenotype in normal and radiation-induced lung injury conditions[J]. Appl Immunohistochem Mol Morphol, 2016, 24(4):283-295.
16	Gao P, Yang J, Gao X, et al. Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling[J]. Mol Med Rep, 2015, 12(2):1971-1976.
17	Liu AR, Liu L, Chen S, et al. Activation of canonical wnt pathway promotes differentiation of mouse bone marrow-derived MSCs into type II alveolar epithelial cells, confers resistance to oxidative stress, and promotes their migration to injured lung tissuein vitro[J]. J Cell Physiol, 2013, 228(6):1270-1283.
18	Cai SX, Liu AR, Chen S, et al. The Orphan receptor tyrosine kinase ROR2 facilitates MSCs to repair lung injury in ARDS animal model[J]. Cell Transplant, 2016, 25(8):1561-1574.
19	Cai SX, Liu AR, Chen S, et al. Activation of Wnt/beta-catenin signalling promotes mesenchymal stem cells to repair injured alveolar epithelium induced by lipopolysaccharide in mice[J]. Stem Cell Res Ther, 2015, 6:65.
20	Cerrada A, de la Torre P, Grande J, et al. Human decidua-derived mesenchymal stem cells differentiate into functional alveolar type II-like cells that synthesize and secrete pulmonary surfactant complexes[J]. PLoS One, 2014, 9(10):e110195.
21	Mendez JJ, Ghaedi M, Steinbacher D, et al. Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds[J]. Tissue Eng. Part A, 2014, 20(11-12):1735-1746.
22	Berger MJ, Adams SD, Tigges BM, et al. Differentiation of umbilical cord blood-derived multilineage progenitor cells into respiratory epithelial cells[J]. Cytotherapy, 2006, 8(5):480-487.
23	Phinney DG. Functional heterogeneity of mesenchymal stem cells: implications for cell therapy[J]. J Cell Biochem, 2012, 113(9):2806-2812.
24	Dominici M, Le Blanc K, Mueller I, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement[J]. Cytotherapy, 2006, 8(4):315-317.
25	Wilson NK, Kent DG, Buettner F, et al. Combined single-cell functional and gene expression analysis resolves heterogeneity within stem cell populations[J]. Cell Stem Cell, 2015, 16(6):712-724.
26	Collins JJ, Thebaud B. Progenitor cells of the distal lung and their potential role in neonatal lung disease[J]. Birth Defects Res A Clin Mol Teratol, 2014, 100(3):217-226.
27	Zhang M, Shi J, Huang Y, et al. Expression of canonical WNT/beta-CATENIN signaling components in the developing human lung[J]. BMC Dev Biol, 2012, 12:21.
28	Liu A, Chen S, Cai S, et al. Wnt5a through noncanonical Wnt/JNK or Wnt/PKC signaling contributes to the differentiation of mesenchymal stem cells into type II alveolar epithelial cellsin vitro[J]. PLoS One, 2014,9(3):e90229.
29	石莉，竭晶，王芳,等. Wnt信号途径促进脂肪间充质干细胞向Ⅱ型肺泡上皮细胞分化[J].中国组织工程研究, 2015, 19(32):5148-5154.
30	Wang X, Wang Y, Snitow ME, et al. Expression of histone deacetylase 3 instructs alveolar type I cell differentiation by regulating a Wnt signaling niche in the lung[J]. Dev Biol, 2016, 414(2):161-169.
31	Sun Z, Gong X, Zhu H, et al. Inhibition of Wnt/beta-catenin signaling promotes engraftment of mesenchymal stem cells to repair lung injury[J]. J Cell Physiol, 2014, 229(2):213-224.
32	Wang C, Zhu H, Sun Z, et al. Inhibition of Wnt/beta-catenin signaling promotes epithelial differentiation of mesenchymal stem cells and repairs bleomycin-induced lung injury[J]. Am J Physiol Cell Physiol, 2014, 307(3):C234-C244.
33	Ludtke TH, Farin HF, Rudat C, et al. Tbx2 controls lung growth by direct repression of the cell cycle inhibitor genes Cdkn1a and Cdkn1b[J]. PLoS Genet, 2013, 9(1):e1003189.
34	Rockich BE, Hrycaj SM, Shih HP, et al. Sox9 plays multiple roles in the lung epithelium during branching morphogenesis[J].Proc Natl Acad Sci U S A, 2013, 110(47):E4456-E4464.
35	Nyeng P, Norgaard GA, Kobberup S, et al. FGF10 maintains distal lung bud epithelium and excessive signaling leads to progenitor state arrest, distalization, and goblet cell metaplasia[J]. BMC Dev Biol, 2008, 8:2.
36	Song M, Lv Q, Zhang X, et al. Dynamic tracking human mesenchymal stem cells tropism following smoke inhalation injury in NOD/SCID mice[J].Stem Cells Int, 2016, 2016:1691856.
37	黄丽丽，徐秀萍，张曦文,等.间充质干细胞向肺组织归巢在ARDS治疗中的研究进展[J].中华医学杂志, 2016, 96(13):1063-1065.
38	Li B, Zhang H, Zeng M, et al. Bone marrow mesenchymal stem cells protect alveolar macrophages from lipopolysaccharide-induced apoptosis partially by inhibiting the Wnt/beta-catenin pathway[J].Cell Biol Int, 2015, 39(2):192-200.

研究者	类型	MSC类型	诱导方法	时间	分化方向
Li^[10]	体外	人BMSC	含角化细胞生长因子、转铁蛋白、牛垂体提取物、肾上腺素、三碘甲腺原氨酸、氢化可的松、维甲酸的培养基	3周	ATⅡ
?	?	?	含角化细胞生长因子、转铁蛋白、牛垂体提取物、肾上腺素、三碘甲腺原氨酸、氢化可的松、维甲酸的培养基	?	?
Yan^[11]	体外	小鼠BMSC	射线损伤肺细胞或上清+添加胰岛素生长因子、肝细胞生长因子、成纤维细胞生长因子4的培养基	3周	AT
?	?	?	射线损伤肺细胞或上清+添加胰岛素生长因子、肝细胞生长因子、成纤维细胞生长因子4的培养基	?	?
Li^[12]	体外	人AFSC	先用KOSR+激活素A处理，再于SABM中培养	2周	ATⅡ
Maria^[13]	体外	大鼠BMSC	正常或X线损伤肺组织细胞+小气道上皮细胞培养基，Transwell隔离培养	1周	ATⅡ
Gao^[14]	体外	大鼠BMSC	肺组织匀浆+添加丹酚酸B的培养基	2周	ATⅠ
Liu^[15]	体外	小鼠BMSC	MLE-12细胞+SAGM	1周	ATⅡ
Cai^[16]	体内	小鼠BMSC	BMSC过表达ROR2，移植入脂多糖介导的肺损伤小鼠	2周	ATⅡ
Cai^[17]	体内	小鼠BMSC	BMSC过表达β-catenin，移植入脂多糖介导的肺损伤小鼠	2周	ATⅡ
Cerrada^[18]	体外	人DMSC	小气道上皮细胞生长试剂盒	5天	ATⅡ
Mendez^[19]	体外	人BMSC、人ATMSC	去细胞的肺支架+SAGM	1周	ATⅡ
Berger^[20]	体外	人UCBMSC	SAGM	8天	ATⅡ

研究者	类型	MSC类型	诱导方法	时间	分化方向
Li^[10]	体外	人BMSC	含角化细胞生长因子、转铁蛋白、牛垂体提取物、肾上腺素、三碘甲腺原氨酸、氢化可的松、维甲酸的培养基	3周	ATⅡ
?	?	?	含角化细胞生长因子、转铁蛋白、牛垂体提取物、肾上腺素、三碘甲腺原氨酸、氢化可的松、维甲酸的培养基	?	?
Yan^[11]	体外	小鼠BMSC	射线损伤肺细胞或上清+添加胰岛素生长因子、肝细胞生长因子、成纤维细胞生长因子4的培养基	3周	AT
?	?	?	射线损伤肺细胞或上清+添加胰岛素生长因子、肝细胞生长因子、成纤维细胞生长因子4的培养基	?	?
Li^[12]	体外	人AFSC	先用KOSR+激活素A处理，再于SABM中培养	2周	ATⅡ
Maria^[13]	体外	大鼠BMSC	正常或X线损伤肺组织细胞+小气道上皮细胞培养基，Transwell隔离培养	1周	ATⅡ
Gao^[14]	体外	大鼠BMSC	肺组织匀浆+添加丹酚酸B的培养基	2周	ATⅠ
Liu^[15]	体外	小鼠BMSC	MLE-12细胞+SAGM	1周	ATⅡ
Cai^[16]	体内	小鼠BMSC	BMSC过表达ROR2，移植入脂多糖介导的肺损伤小鼠	2周	ATⅡ
Cai^[17]	体内	小鼠BMSC	BMSC过表达β-catenin，移植入脂多糖介导的肺损伤小鼠	2周	ATⅡ
Cerrada^[18]	体外	人DMSC	小气道上皮细胞生长试剂盒	5天	ATⅡ
Mendez^[19]	体外	人BMSC、人ATMSC	去细胞的肺支架+SAGM	1周	ATⅡ
Berger^[20]	体外	人UCBMSC	SAGM	8天	ATⅡ

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