To decode the heterogeneity of human hematopoietic system in early development of human embryo and its potential regulatory mechanisms.

Bioinformatic methods were used to explore the single-cell transcriptome sequencing data of the earliest human embryo samples so far, including dimensional reduction analysis, the identification and correlation analysis of different cell subsets, the potential regulation of non-hematopoietic cells on hematopoietic system as well as the development pathway and the regulation mechanism of hematopoietic system itself; data visualization is conducted by R programming language and various R and Python packages.

Many cell types were detected in early human embryonic development, and the hematopoietic system could be was traced as early as CS7 stage; non-hematopoietic cells played potential regulatory roles in hematopoietic system through the receptors-ligands; the development of hematopoiesis was accompanied by the attenuation of endothelial cell characteristics and the enhancement of the functions of specific blood cell types; based on this single-cell transcriptome dataset, the heterogeneity of hematopoietic system in the early stage of human embryonic development was revealed and a variety of regulatory factors that plays a role in specific cell types were identified.

Heterogeneitiesof the hematopoietic system in the early human embryo development were identified, and the hematopoietic system may be regulated by other cell types. Potential regulatory factors of specific blood celltypes in this early stage were revealed.