Influence of transplantation with basic fibroblast growth factor gene-transfected bone mesenchymal stem cells on heart function in rats with heart failure

doi:10.3877/cma.j.issn.2095-1221.2017.05.004

Abstract

Abstract:

Objective

To explore the influence of transplantation with basic fibroblast growth factor (bFGF) gene-transfected bone mesenchymal stem cells (BMSCs) on heart function in rats with heart failure.

Methods

A heart failure model of SD rats was induced by intraperitoneal injection with adriamycin. The 33 survived rats were randomly divided into 3 groups: a model group, a BMSCs group and a BMSCs+bFGF group (n= 11 for all). Another 8 rats served as control. BMSCs at the third passage were transfected with bFGF and labeled with BrdU. BMSCs (3×10⁶), bFGF-transfected BMSCs (3×10⁶), or culture medium were respectively transplanted into rats via the tail vein. Cardiac function was evaluated with the use of a physiological recorder. The hearts were analyzed by immunohistochemical staining to identify the transplanted BrdU-labeled cells. The expressions of bFGF protein and mRNA in the heart tissues were respectively determined by Western Blotting and real time PCR. One-Way ANOVA or Fisher's exact test was used in the analysis as appropriately.

Results

Four weeks after transplantation, hemodynamic measurements showed LVSP (121.13±12.28) mmHg, LVDEP (3.86±1.25) mmHg, LV +dP/dt (3671.25±172.50) mmHg/s, LV-dP/dt (3221.63±259.57) mmHg/s in the normal control group; LVSP (81.29±12.39) mmHg, LVDEP (16.43±4.12) mmHg, LV+dP/dt (2344.29±98.63) mmHg/s, LV-dP/dt (2244.29±103.10) mmHg/s in the model group; LVSP (97.00±6.39) mmHg, LVDEP (12.00±2.73) mmHg, LV+dP/dt (2876.25±118.31) mmHg/s, LV-dP/dt (2726.25±303.23) mmHg/s in the BMSCs group, and LVSP (109.38±12.78) mmHg, LVDEP (9.25±1.67) mmHg, LV+dP/dt (3037.50±161.22) mmHg/s, LV-dP/dt (3000.13±149.77) mmHg/s in the BMSCs+bFGF group. The parameters were all significantly improved (F= 17.29, 36.61, 111.11 and 26.54 respectively, Pall < 0.01) after the transplantation. BrdU-labeled transplanted BMSCs could be found in the hearts of the recipients. The expression of bFGF mRNA in the heart tissues were significantly increased in the model group (1.28±0.06) than in the control group (t= 10.46, P< 0.01); in the BMSCs group (1.37±0.05) than in the model group (t?= 3.39,P< 0.01); and in the BMSCs+bFGF group (1.44±0.03) than in the BMSCs group (t=2.86,P= 0.01). As shown by Western Blotting test, the bFGF protein expression level of the heart tissues was the lowest in the normal control, but significantly higher in the model group than in the control group (1.31±0.09vs1.00±0.00,t= 5.48,P= 0.01), in the BMSCs group than in the model group (1.46?±0.05vs1.31±0.09,t= 2.68,P= 0.03), and in the BMSCs+bFGF group than in the BMSCs group (1.84±0.09vs1.46±0.05,t= 6.79,P< 0.01).

Conclusion

Transplantation with BMSCs or bFGF-transfected BMSCs can both improve cardiac function in rats with adriamycin-induced heart failure, while the effect of bFGF-transfected BMSCs may be better than BMSCs only.

Key words: Basic fibroblast growth factor, Mesenchymal stem cells, Cell transplantation, Heart failure

Zenghua Lai, Xuekai Dou, Chunwen Jia, Chaohui Jiang. Influence of transplantation with basic fibroblast growth factor gene-transfected bone mesenchymal stem cells on heart function in rats with heart failure[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2017, 07(05): 271-276.

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References 20

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分组	n	LVSP（mmHg）	LVDEP（mmHg）	LV+dP/dt（mmHg/s）	LV-dP/dt（mmHg/s）
正常对照组	8	121.13±12.28	3.86±1.25	3671.25±172.50	3221.63±259.57
模型组	7	81.29±12.39^a	16.43±4.12^a	2344.29±98.63^a	2244.29±103.10^a
BMSCs组	8	97.00±6.39^ab	12.00±2.73^ab	2876.25±118.31^ab	2726.25±303.23^ab
BMSCs+bFGF组	8	109.38±12.78^abc	9.25±1.67^abc	3037.50±161.22^abc	3000.13±149.77^bc
F值	?	17.29	36.61	111.11	26.54
P值	?	< 0.01	< 0.01	< 0.01	< 0.01

分组	n	LVSP（mmHg）	LVDEP（mmHg）	LV+dP/dt（mmHg/s）	LV-dP/dt（mmHg/s）
正常对照组	8	121.13±12.28	3.86±1.25	3671.25±172.50	3221.63±259.57
模型组	7	81.29±12.39^a	16.43±4.12^a	2344.29±98.63^a	2244.29±103.10^a
BMSCs组	8	97.00±6.39^ab	12.00±2.73^ab	2876.25±118.31^ab	2726.25±303.23^ab
BMSCs+bFGF组	8	109.38±12.78^abc	9.25±1.67^abc	3037.50±161.22^abc	3000.13±149.77^bc
F值	?	17.29	36.61	111.11	26.54
P值	?	< 0.01	< 0.01	< 0.01	< 0.01

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