Curative effect of transplantion of cardiomyocyte-like cells on myocardial infarction rats

doi:10.3877/cma.j.issn.2095-1221.2017.04.003

Abstract

Abstract:

Objective

To observe the effect of transplanting cardiomyocyte-like cells (CLC) to treat myocardial infarction in rats.

Methods

Animal models of myocardial infarction were prepared, and hemodynamics changes of rats models were tested by ultrasonic method 4 weeks later. Bone mesenchymal stem cells (BMSC) were isolated and purified in vitro, which were induced to CLCs by 15 ng/ml insulin-like growth factor 1 (IGF-1) , and then the expression of myocardial iconic protein α-SMA, Troponin-T and Troponin-Ⅰwere detected with Western blotting method in 3 d, 7 d, 14 d and 21 d. The experimental rats were randomly assigned into 4 groups (a myocardial infarction group, a normal saline group, a BMSC group and a CLC group) . The area of myocardial infarction was detected on rats 4 weeks after transplantation therapy by HE coloring. Data among multiple groups were compared by one-way ANOVA and data between two independent groups were compared by t-test.

Results

After myocardial infarction models were prepared for 4 weeks, the LVSP (118.74 ± 11.56) mmHg of the model group were significantly lower than those of the sham operation group (130.42 ± 12.58) mmHg, the LVEDP (14.29 ± 7.63) mmHg of the model group were significantly higher than those of the sham operation group (2.44 ±1.11) mmHg, +dp/dt (2645.17 ± 468.16) mmHg/s of the model group were significantly lower than those of the sham group (4087.29 ± 525.17) mmHg/s, and -dp/dt (2487.80 ± 348.21) mmHg/s of the model group were also significantly lower than those of the sham group (3865.81 ± 614.37) mmHg/s (P < 0.01) . The expressions of α-SMA, Troponin-T and Troponin-Ⅰwere detected when BMSCs were induced by IGF-1 on 3 d, 7 d, 14 d and 21 d (P < 0.01) . There were not any changes in haemodynamics of the normal saline group 4 weeks after transplantation therapy, the LVEDP of the BMSC group (9.15?±?4.54) mmHg and CLC group (4.67 ± 3.46) mmHg were significantly lower than those of the myocardial infarction group (14.29 ± 7.63) mmHg, the +dp/dt of BMSC group (3245.45?±?368.76) mmHg/s and CLC group (3612.78?±?468.54) mmHg/s were significantly higher than those of the myocardial infarction group (2645.17?±?468.16) mmHg/s, the -dp/dt of BMSC group (3014.11?±?412.12) mmHg/s and CLC group (3347.21?±?398.56) mmHg/s were also significantly higher than the sham group (2487.80?±?348.21) mmHg/s. In the meantime, the LVEDP of the CLC group (4.67?±?3.46) mmHg was significantly lower than that of the BMSC group (9.15?±?4.54) mmHg P < 0.01, the +dp/dt of the CLC group (3612.78?±?468.54) mmHg/s were significantly higher than those of the BMSC group (3245.368?±?468.16) , the -dp/dt of the CLC group (3347.21?±?398.56) mmHg/s were also significantly higher than those of the BMSC group (3014.11?±?412.12) mmHg/s. The myocardial infarction area of the BMSC group and CLC group was reduced obviously compared with the myocardial infarction group, and the myocardial infarction area of CLC group was less than that of the BMSC group (P < 0.01) .

Conclusion

It may be of a better effect that transplanting BMSC induced by IGF-1 into CLC than only transplanting BMSC on myocardial infarction rats.

Key words: Bone marrow, Mesenchymal stem cells, Insulin-like growth factor I, Myocytes, cardiac, Myocardial infarction, Cell transplantation

Xiuli Wang, Shiqiang Jiang, Ying Liu, Jie Wang, Qian Lyu, Lie Wang, Chunmei Li, Haibin Gong. Curative effect of transplantion of cardiomyocyte-like cells on myocardial infarction rats[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2017, 07(04): 202-207.

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Figures/Tables 6

References 16

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分组	动物数	LVSP（mmHg）	LVEDP（mmHg）	+dp/dt（mmHg/s）	-dp/dt（mmHg/s）
假手术组	30	130.42±12.58	2.44±1.11	4087.29±525.17	3865.81±614.37
模型组	40	118.74±11.56	14.29±7.63	2645.17±468.16	2487.80±348.21
t值	?	3.979	-9.605	11.905	11.028
P值	?	< 0.01	< 0.01	< 0.01	< 0.01

分组	动物数	LVSP（mmHg）	LVEDP（mmHg）	+dp/dt（mmHg/s）	-dp/dt（mmHg/s）
假手术组	30	130.42±12.58	2.44±1.11	4087.29±525.17	3865.81±614.37
模型组	40	118.74±11.56	14.29±7.63	2645.17±468.16	2487.80±348.21
t值	?	3.979	-9.605	11.905	11.028
P值	?	< 0.01	< 0.01	< 0.01	< 0.01

分组	第3天	第7天	第14天	第21天	F值	P值
α-SMA	1.00±0.00	1.14±0.08	1.58±0.09	1.60±0.05	250.69	< 0.01
Troponin-T	1.00±0.00	1.35±0.06	1.92±0.10	1.85±0.07	445.55	< 0.01
Troponin-I	1.00±0.00	1.17±0.26	1.89±0.08	1.89±0.10	106.36	< 0.01

分组	第3天	第7天	第14天	第21天	F值	P值
α-SMA	1.00±0.00	1.14±0.08	1.58±0.09	1.60±0.05	250.69	< 0.01
Troponin-T	1.00±0.00	1.35±0.06	1.92±0.10	1.85±0.07	445.55	< 0.01
Troponin-I	1.00±0.00	1.17±0.26	1.89±0.08	1.89±0.10	106.36	< 0.01

分组	动物数	LVSP（mmHg）	LVEDP（mmHg）	+dp/dt（mmHg/s）	-dp/dt（mmHg/s）
心肌梗死组	10	118.74±11.56	14.29±7.63	2645.17±468.16	2487.80±348.21
生理盐水对照组	10	119.67±10.41	14.12±3.89	2754.24±478.25	2498.14±354.34
BMSC组	10	121.25± 9.45	9.15±4.54^a	3245.45±368.76^a	3014.11±412.12^a
CLC组	10	125.14± 6.78	4.67±3.46^ab	3612.78±468.54^ab	3347.21±398.56^ab
F值	?	1.023	42.441	14.367	13.758
P值	?	0.393	< 0.01	< 0.01	< 0.01

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