Explore the best biomarker method to track cells and better observe the homogenization of cells after transplantation in the body.

Four cells were transfected with a novel lentivirus (eGFP+PURO Lentivirus) . The optimal MOI of the four cells was determined. After the cells were successfully transfected, the cells were screened with the optimum concentration of puromycin. Four stable GFP-positive cell lines were obtained. The cells used included human umbilical cord mesenchymal stem cells (hUC-MSC) , tree shrew umbilical cord mesenchymal stem cells (TS-UC-MSC) , human embryonic kidney cells (293T) , C57BL mouse bone marrow mesenchymal stem cells (C57-BMSC) .

Four kinds of common cells were successfully transfected with novel lentivirus (eGFP + PURO Lentivirus) . The success rate of transfection was 100﹪. The cell was successfully labeled with GFP fluorescence and the optimal concentration of puromycin and MOI value was determined. The optimal MOI of TS-UC-MSC was 240, the optimal MOI of hUC-MSC was 150, and the optimal MOI of 293T was 100, the optimal MOI of C57-BMSC was 50. C57BL mice were transfused with C57-BMSC cells labeled by GFP. Frozen sections showed labeled cells were in the liver. The labeled cells were not found in the spleen, lung, kidney and heart, indicating that the foreign cells were mainly metabolized in the liver.

In cell transplantation treatment, the cells successfully labeled GFP can be traced in animals, a good cell tracer method can be a good reflection of the study of cells in vitro and in vivo trends.