Effects of human umbilical cord blood mononuclear cells in a rat model of Parkinson's disease

doi:10.3877/cma.j.issn.2095-1221.2017.02.002

Abstract

Abstract:

Objective

To explore whether human umbilical cord blood mononuclear cells (HUCB-MNC) transplantation could treat Parkinson's disease (PD) .

Methods

6-OHDA-lesioned PD rats were divided into two groups randomly: one group was treated with HUCB-MNCs, which were isolated from umbilical cord and the other group was treated with PBS as control. Apomorphine-induced rotation test was used to evaluate the motor behavior of the rats at different times after cell transplantation. Immunochemistry was conducted to confirm the differentiation of the grafted cells in the brain.

Results

The rates of apomorphine-induced rotation were 0.529±0.121, 0.457±0.096, 0.369±0.089, and 0.264±0.091 at 4, 8, 12, and 16 weeks after transplantation respectively, which were significantly lower than those of the control group (0.843±0.158, 0.812±0.156, 0.736±0.103, and 0.714±0.160 respectively) . And the duration time on rotation rod of the treatment group were (46.333±2.082) s, (63.667±4.041) s, (76.667±7.638) s, and (126±12.166) s respectively for each time points, while those of the control group were (29.250±5.560) s, (26.750±8.655) s, (34.250±10.905) s, and (47.500±6.856) respectively. Immunochemistry assay showed that the grafted HUCB-MNC expressed neuron maker Tuj1 and dopamine neuron maker TH.

Conclusion

Our study shows that transplanted HUCB-MNCs may differentiate into neurons and dopamine neurons and alleviate motor dysfunction of PD rats.

Key words: Cord blood stem cell transplantation, Monocytes, Parkinson disease, Cell differentiation, Dopamine, Neurons

Chao Chen, Jing Duan, Aifang Shen, Wei Wang, Hao Song, Xianjie Lu, Xiaobing Wang, Zhongchao Han, Fabin Han. Effects of human umbilical cord blood mononuclear cells in a rat model of Parkinson's disease[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2017, 07(02): 71-76.

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References 22

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分组	只数	4周	8周	12周	16周
对照组	7	0.843±0.158	0.812±0.156	0.736±0.103	0.714±0.160
移植组	7	0.529±0.121	0.457±0.096	0.369±0.089	0.264±0.091
t值	?	4.377	5.561	6.835	6.071
P值	?	< 0.01	< 0.01	< 0.01	< 0.01

分组	只数	4周	8周	12周	16周
对照组	7	0.843±0.158	0.812±0.156	0.736±0.103	0.714±0.160
移植组	7	0.529±0.121	0.457±0.096	0.369±0.089	0.264±0.091
t值	?	4.377	5.561	6.835	6.071
P值	?	< 0.01	< 0.01	< 0.01	< 0.01

分组	只数	4周	8周	12周	16周
对照组	7	29.250±5.560	26.750±8.655	34.250±10.905	47.500± 6.856
移植组	7	46.333±2.082	63.667±4.041	76.667± 7.638	126.000±12.166
t值	?	4.966	6.189	5.707	10.990
P值	?	< 0.01	< 0.01	< 0.01	< 0.01

分组	只数	4周	8周	12周	16周
对照组	7	29.250±5.560	26.750±8.655	34.250±10.905	47.500± 6.856
移植组	7	46.333±2.082	63.667±4.041	76.667± 7.638	126.000±12.166
t值	?	4.966	6.189	5.707	10.990
P值	?	< 0.01	< 0.01	< 0.01	< 0.01

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