切换至 "中华医学电子期刊资源库"
高级检索
中华细胞与干细胞杂志(电子版)

中华细胞与干细胞杂志(电子版) ›› 2023, Vol. 13 ›› Issue (01) : 1 -9. doi: 10.3877/cma.j.issn.2095-1221.2023.01.001

论著

芒柄花黄素通过调控miR-140-5p/TIGIT轴影响喉鳞状细胞癌细胞增殖和凋亡
武川军1, 彭丽娜1,†(), 韩海平1, 冯志星1   
  1. 1. 056002　邯郸，河北省邯郸市中心医院耳鼻咽喉科
  • 收稿日期:2022-10-31 出版日期:2023-02-01
  • 通信作者: 彭丽娜

Effect of formononetin on proliferation and apoptosis of laryngeal squamous cell carcinoma cells and its possible mechanism

Chuanjun Wu1, Lina Peng1,†(), Haiping Han1, Zhixing Feng1   

  1. 1. Department of Otolaryngology, Handan Central Hospital, Handan 056002, China
  • Received:2022-10-31 Published:2023-02-01
  • Corresponding author: Lina Peng
全文 ( ) 下载PDF ( ) 导出引用
引用本文：

武川军, 彭丽娜, 韩海平, 冯志星. 芒柄花黄素通过调控miR-140-5p/TIGIT轴影响喉鳞状细胞癌细胞增殖和凋亡[J]. 中华细胞与干细胞杂志(电子版), 2023, 13(01): 1-9.

Chuanjun Wu, Lina Peng, Haiping Han, Zhixing Feng. Effect of formononetin on proliferation and apoptosis of laryngeal squamous cell carcinoma cells and its possible mechanism[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2023, 13(01): 1-9.

目的

探讨芒柄花黄素对喉鳞状细胞癌细胞增殖、凋亡的影响及其可能的机制。

方法

体外培养人喉鳞状细胞癌细胞系Hep-2，以MTT法检测0、5、10、20、40、60 μmol/L芒柄花黄素处理24 h后Hep-2细胞活力，根据IC50值筛选出芒柄花黄素合适作用浓度。Hep-2细胞分别采用20、40 μmol/L芒柄花黄素，40 μmol/L芒柄花黄素+阴性对照（转染mirRNA-140-5p抑制剂阴性对照），40 μmol/L芒柄花黄素+miR-140-5p inhibitor干预细胞敲减干预Hep-2细胞。分组转染及药物处理后，采用实时荧光定量PCR （qRT-PCR）实验和免疫印迹检测Hep-2细胞miR-140-5p与T细胞免疫球蛋白和ITIM结构域蛋白（TIGIT）表达；采用MTT法和平板集落形成实验检测Hep-2细胞增殖；采用流式细胞实验检测Hep-2细胞凋亡；采用免疫印迹检测Hep-2细胞增殖（PCNA、Cyclin D1）与凋亡相关蛋白（Bax、cleaved caspase-3）表达。于裸鼠背部皮下注射Hep-2细胞构建裸鼠移植瘤模型，随机分为对照组，25、50 mg/kg芒柄花黄素组，50 mg/kg芒柄花黄素+阴性对照组，50 mg/kg芒柄花黄素+ miR-140-5p敲减组，分组处理后，检测各组裸鼠移植瘤质量与体积；采用双荧光素酶报告实验分析Hep-2细胞miR-140-5p对TIGIT的靶向调控。两组间比较采用t检验，多组间比较采用单因素方差分析，进一步两两比较采用SNK-q检验。

结果

与对照比较，20、40 μmol/L芒柄花黄素干预细胞miR-140-5p表达（1.79±0.16、2.58±0.22比1.04±0.11）、凋亡率[（36.17±8.14）%、（68.65±14.20）%比（2.10±0.65）%]、Bax与cleaved caspase-3蛋白表达升高（P < 0.05），TIGIT蛋白（0.56±0.12、0.17±0.01比0.98±0.10）与mRNA表达（0.68±0.10、0.34±0.03比1.05±0.13）、集落生成率[（60.82±12.24）%、（35.36±8.20）%比（100.0±0.00）%]、细胞活力（63.12±11.03、39.75±7.14比100.0±0.00）、PCNA与Cyclin D1蛋白表达降低（P < 0.05）；与40 μmol/L芒柄花黄素比较，芒柄花黄素+miR-140-5p敲减细胞miR-140-5p表达（1.11±0.13比2.58±0.22）、凋亡率[（5.04±1.51）%比（68.65±14.20）%]、Bax与cleaved caspase-3蛋白表达降低（P < 0.05），TIGIT蛋白（0.92±0.18比0.17±0.01）与mRNA表达（0.97±0.14比0.34±0.03）、集落生成率[（90.76±15.02）%比（35.36±8.20）%]、细胞活力（92.01±17.13比39.75±7.14）、PCNA与Cyclin D1蛋白表达升高（P < 0.05）；动物实验中，与对照组比较，25、50 mg/kg芒柄花黄素组裸鼠移植瘤质量[（609.23±58.14）、（465.87±41.26）比（762.14±76.51） mg]降低，体积[（532.82±60.28）、（396.34±48.10）比（681.70±72.44）mm3]减小（P < 0.05）；与50 mg/kg芒柄花黄素组比较，芒柄花黄素+miR-140-5p敲减组裸鼠移植瘤质量[（735.48±80.12）比（465.87±41.26）mg]升高，体积[（654.79±75.85）比（396.34±48.10） mm3]增加（P < 0.05）；Hep-2细胞miR-140-5p可靶向下调TIGIT表达。

结论

芒柄花黄素通过调控miR-140-5p/TIGIT轴表达抑制喉鳞状细胞癌细胞增殖活性，并促进其凋亡。

关键词: 芒柄花黄素, miR-140-5p/TIGIT, 喉鳞状细胞癌细胞, 增殖, 凋亡
Objective

To investigate the effect of formononetin on the proliferation and apoptosis of laryngeal squamous cell carcinoma cells and its possible mechanism.

Methods

The human laryngeal squamous cell carcinoma cell line Hep-2 was cultured in vitro, and the cell viability of Hep-2 cells was detected by the MTT method after treatment with 0, 5, 10, 20, 40, 60 μmol/L formononetin for 24 h. The appropriate concentration of mancoxanthin was screened according to the IC50 value. Hep-2 cells cultured were treated with, 20 μmol/L formononetin (20 μmol/L) , 40 μmol/L formononetin (40 μmol/L) , formononetin (40 μmol/L) + negative control (transfected with miR-140-5p inhibitor negative control) , formononetin (40 μmol/L) + miR-140-5p inhibitor (transfected with miR-140-5p inhibitor) . After transfection and drug treatment, the expression of miR-140-5p and T cell immunoglobulin and ITIM domain protein (TIGIT) in Hep-2 cells in each group was detected by real-time fluorescence quantitative PCR (qRT-PCR) experiment and western blot; the proliferation of Hep-2 cells in each group was detected by MTT assay and plate colony formation assay; the apoptosis of Hep-2 cells in each group was detected by flow cytometry; Western blot was used to detect the proliferation (PCNA, Cyclin D1) and apoptosis-related protein (Bax, cleaved caspase-3) expression of Hep-2 cells in each group. Nude mice were subcutaneously injected with Hep-2 cells on the back to establish a transplanted tumor model in nude mice, and they were randomly grouped into a control group, a low-dose formononetin (25 mg/kg) group, a high-dose formononetin (50 mg/kg) group, high-dose formononetin (50 mg/kg) + negative control (transfected with miR-140-5p antagomir negative control) group, high dose of forsythia (50 mg/kg) + miR-140-5p knockdown (transfected with miR-140-5p antagomir) group. After treatment, the quality and volume of transplanted tumors in nude mice of each group were detected; a dual-luciferase reporter assay was used to analyze the targeted regulation of TIGIT by miR-140-5p in Hep-2 cells.

Results

Compared with the control, the expression of miR-140-5p (1.79±0.16, 2.58±0.22 vs 1.04±0.11) , the apoptosis rate [ (36.17±8.14) %、(68.65±14.20) %vs (2.10±0.65) %], the protein expression of Bax and cleaved caspase-3 were increased, in the 20 μmol/L formononetin and the 40 μmol/L formononetin treated cells (P < 0.05) , the expression of TIGIT protein (0.56±0.12, 0.17±0.01 vs 0.98±0.10) and mRNA (0.68±0.10, 0.34±0.03 vs 1.05±0.13) , colony formation rate [ (60.82±12.24) %、(35.36±8.20) %vs (100.0±0.00) %], cell viability (63.12±11.03, 39.75±7.14 vs 100.0±0.00) , protein expression of PCNA and Cyclin D1 were decreased (P < 0.05) ; compared with the 40 μmol/L formononetin, the expression of miR-140-5p (1.11±0.13 vs 2.58± 0.22) , the apoptosis rate [ (5.04±1.51) %vs (68.65±14.20) %], the protein expression of Bax and cleaved caspase-3 were decreased in the high-dose formononetin+miR-140-5p knockdown cells (P < 0.05) , the expression of TIGIT protein (0.92±0.18 vs 0.17±0.01) and mRNA (0.97±0.14 vs 0.34±0.03) , colony formation rate[ (90.76±15.02) %vs (35.36±8.20) %], cell viability (92.01±17.13 vs 39.75±7.14) , protein expression of PCNA and Cyclin D1 were increased (P < 0.05) ; Compared with the control group, the weight [ (609.23±58.14) 、(465.87±41.26) vs (762.14±76.51) mg] and volume [ (532.82±60.28) 、(396.34±48.10) vs (681.70±72.44) mm3] of transplanted tumor were decreased in nude mice in the low-dose formononetin group and the high-dose formononetin group (P < 0.05) ; Compared with the high-dose formononetin group, the weight [ (735.48±80.12) vs (465.87±41.26) mg] and volume [ (654.79±75.85) vs (396.34±48.10) mm3] of transplanted tumor were increased in nude mice in the high-dose formononetin+miR-140-5p knockdown group (P < 0.05) ; MiR-140-5p in Hep-2 cells targeted to down-regulate TIGIT expression.

Conclusion

Formononetin inhibits proliferative activity and promotes apoptosis in laryngeal squamous cell carcinoma cells by regulating miR-140-5p/TIGIT axis expression.

Key words: Formononetin, miR-140-5p/TIGIT, Laryngeal squamous cell carcinoma cell, Proliferation, Apoptosis
表1 各因引物序列
引物名称 序列（5'-3'） 预期扩增产物长度（bp）
miR-140-5p 上游ACACTCCAGCTGGGCAGTGGTTTTACCCTA 102
  下游TGGTGTCGTGGAGTCG  
U6 上游GCTTCGGCAGCACATATACTAAAAT 91
  下游CGCTTCACGAATTTGCGTGTCAT  
TIGIT 上游TATCCTTCCACCATCTCTCTCA 110
  下游CACAACCTTGCTTCACATTCTC  
β-actin 上游GCTCCCCGGGCTGTATTC 76
  下游CCTTCTGACCCATTCCCACC  
图1 不同浓度芒柄花黄素对Hep-2细胞活力的影响注：与0 μmol/L芒柄花黄素比较，aP < 0.05
图2 免疫印迹检测芒柄花黄素对Hep-2细胞TIGIT蛋白表达注：1为对照；2为20 μmol/L芒柄花黄素；3为40 μmol/L芒柄花黄素；4为芒柄花黄素+阴性对照；5为芒柄花黄素+miR-140-5p inhibitor
表2 芒柄花黄素对Hep-2细胞miR-140-5p与TIGIT的表达影响（ ± s）
分组 实验次数 RNA 蛋白
miR-140-5p/U6 TIGIT/β-actin TIGIT/β-actin
对照 3 1.04±0.11 1.05±0.13 0.98±0.10
20μmol/L芒柄花黄素 3 1.79±0.16a 0.68±0.10a 0.56±0.12a
40μmol/L芒柄花黄素 3 2.58±0.22ab 0.34±0.03ab 0.17±0.01ab
芒柄花黄素+阴性对照 3 2.60±0.19 0.35±0.06 0.16±0.05
芒柄花黄素+miR-140-5p敲减 3 1.11±0.13c 0.97±0.14c 0.92±0.18c
F值   123.975 65.791 78.040
P值   < 0.001 < 0.001 < 0.001
图3 显微镜下观察芒柄花黄素对Hep-2细胞集落生成（结晶紫染色）注：a为对照；b为20 μmol/L芒柄花黄素；c为40 μmol/L芒柄花黄素；d为芒柄花黄素+阴性对照；e为芒柄花黄素+miR-140-5p敲减
图4 流式细胞术检测芒柄花黄素对Hep-2细胞凋亡情况注：a为对照；b为20 μmol/L芒柄花黄素；c为40 μmol/L芒柄花黄素；d为芒柄花黄素+阴性对照；e为芒柄花黄素+miR-140-5p敲减
表3 芒柄花黄素对Hep-2细胞的凋亡率、集落生成率、细胞活力的影响（ ± s）
分组 实验次数 细胞凋亡率（%） 集落生成率（%） 细胞活力（%）
对照 3 2.10±0.65 100.0±0.00 100.0±0.00
20 μmol/L芒柄花黄素 3 36.17±8.14a 60.82±12.24a 63.12±11.03a
40 μmol/L芒柄花黄素 3 68.65±14.20ab 35.36±8.20ab 39.75±7.14ab
芒柄花黄素+阴性对照 3 67.13±15.14 32.40±5.03 40.14±8.01
芒柄花黄素+miR-140-5p敲减 3 5.04±1.51c 90.76±15.02c 92.01±17.13c
F值   62.163 61.559 45.202
P值   < 0.001 < 0.001 < 0.001
图5 免疫印迹检测芒柄花黄素对Hep-2细胞增殖与凋亡相关蛋白的表达注：1为对照；2为20 μmol/L芒柄花黄素；3为40 μmol/L芒柄花黄素；4为芒柄花黄素+阴性对照；5为芒柄花黄素+miR-140-5p敲减
表4 芒柄花黄素对Hep-2细胞增殖与凋亡相关蛋白表达的影响（ ± s）
分组 实验次数 Cyclin D1/β-actin PCNA/β-actin Bax/β-actin cleaved caspase-3/β-actin
对照 3 1.13±0.20 0.94±0.14 0.22±0.03 0.30±0.04
20 μmol/L芒柄花黄素 3 0.64±0.11a 0.50±0.09a 0.64±0.08a 0.71±0.10a
40 μmol/L芒柄花黄素 3 0.17±0.02ab 0.08±0.01ab 1.06±0.16ab 1.13±0.18ab
芒柄花黄素+阴性对照 3 0.15±0.04 0.09±0.01 1.04±0.18 1.14±0.20
芒柄花黄素+miR-140-5p敲减 3 1.07±0.15c 0.88±0.12c 0.26±0.04c 0.34±0.06c
F值   86.718 121.007 73.668 56.962
P值   < 0.001 < 0.001 < 0.001 < 0.001
图6 芒柄花黄素对Hep-2细胞裸鼠移植瘤的影响注：给药后第15天解剖取出的移植瘤大小，n = 10，n为动物数
表5 芒柄花黄素对Hep-2细胞裸鼠移植瘤质量与体积的影响（ ± s，n = 10）
分组 移植瘤质量（mg） 移植瘤体积（mm3）
对照组 762.14±76.51 681.70±72.44
25 mg/kg芒柄花黄素组 609.23±58.14a 532.82±60.28a
50 mg/kg芒柄花黄素组 465.87±41.26ab 396.34±48.10ab
芒柄花黄素+阴性对照组 478.30±44.60 412.06±50.32
芒柄花黄素+miR-140-5p敲减组 735.48±80.12c 654.79±75.85c
F值 29.857 27.033
P值 < 0.001 < 0.001
图7 miR-140-5p与TIGIT之间存在结合位点
表6 miR-140-5p对野生TIGIT和突变TIGIT荧光素酶活性的影响（ ± s，n = 3）
分组 野生TIGIT 突变TIGIT
miR-140-5p mimics阴性对照 1.04±0.17 1.01±0.19
miR-140-5p mimics 0.32±0.03 0.96±0.22
t值 10.216 0.421
P值 < 0.001 0.682
1
Mattioli F, Fermi M, Molinari G, et al. pT3 N0 laryngeal squamous cell carcinoma: oncologic outcomes and prognostic factors of surgically treated patients[J]. Laryngoscope. 2021, 131(10):2262-2268.
2
Ouban A. Filamin-A expression in laryngeal squamous cell carcinoma and its clinical significance[J]. Histol Histopathol. 2022, 37(2):125-136.
3
Mahajan M, Sitasawad S. miR-140-5p attenuates hypoxia-induced breast cancer progression by targeting Nrf2/HO-1 axis in a keap1-independent mechanism[J]. Cells. 2021, 11(1):12-30.
4
Kase-Kato I, Asai S, Minemura C, et al. Molecular pathogenesis of the coronin family: CORO2A facilitates migration and invasion abilities in oral squamous cell carcinoma[J]. Int J Mol Sci. 2021, 22(23):12684-12700.
5
Wang Y, Huang Q, Li F. miR-140-5p targeted FGF9 and inhibited the cell growth of laryngeal squamous cell carcinoma[J]. Biochem Cell Biol. 2020, 98(2):83-89.
6
Ishihara S, Iwasaki T, Kohashi K, et al. Clinical significance of signal regulatory protein alpha and T cell immunoreceptor with immunoglobulin and immunoreceptor tyrosine-based inhibition motif domain expression in undifferentiated pleomorphic sarcoma[J]. J Cancer Res Clin Oncol. 2022. Online ahead of print.
7
Liu X, Li Q, Zhou Y, et al. Dysfunctional role of elevated TIGIT expression on T cells in oral squamous cell carcinoma patients[J]. Oral Dis. 2021, 27(7):1667-1677.
8
赵玉民, 冯叶雯, 张黎, 等. 芒柄花素抗肿瘤作用机制的研究进展[J]. 中国实验方剂学杂志, 2021, 27(10):193-203.
9
陈庆, 李海虹, 欧伟宁, 等. 芒柄花黄素对人肾癌细胞786-O的抑制作用及机制研究[J]. 中国医药导报, 2021, 18(12):11-1419.
10
刁玉佩, 李萍, 郭明坤, 等. 芒柄花黄素对喉癌TU686细胞生长,增殖和凋亡的影响[J]. 临床与实验病理学杂志, 2021, 37(10):1207-1212.
11
张瑞芳, 侯继崇, 张玲娟. 地龙胶囊联合放疗对喉鳞癌裸鼠移植瘤血管生成的抑制作用[J]. 广州中医药大学学报, 2022, 39(5):1151-1155.
12
Zhou Q, Zhang W, Li T, et al. Formononetin enhances the tumoricidal effect of everolimus in breast cancer MDA-MB-468 cells by suppressing the mTOR pathway[J]. Evid Based Complement Alternat Med. 2019, 2019:9610629.
13
Gao K, Zhu Y, Wang H, et al. Network pharmacology reveals the potential mechanism of Baiying Qinghou decoction in treating laryngeal squamous cell carcinoma[J]. Aging (Albany NY). 2021, 13(24):26003-26021.
14
Daquan W, Tian W, Shen N, et al. Decrement of prognostic nutrition index in laryngeal diseases: from precancerous lesion to squamous cell carcinoma[J]. Acta Otolaryngol. 2021, 141(12):1070-1074.
15
柳扬, 龚毅, 范伟. 构建靶向型Pluronic F127/芒柄花黄素纳米复合体系体外抗肝癌活性[J]. 中国组织工程研究, 2021, 25(4):526-531.
16
黄寅, 蒋智军, 徐卫红. 芒柄花黄素通过circ_0020123/miR-145对胰腺癌细胞增殖迁移侵袭的影响[J]. 中国药师, 2021, 24(8):493-499.
17
Huang B, Li Y, Deng Z, et al. Regulatory mechanism of lncRNA CTBP1-AS2 in nasopharyngeal carcinoma cell proliferation and apoptosis via the miR-140-5p/BMP2 axis[J]. Protein Pept Lett. 2022. Online ahead of print.
18
Wan L, Gu D, Li P. LncRNA SNHG16 promotes proliferation and migration in laryngeal squamous cell carcinoma via the miR-140-5p/NFAT5/Wnt/β-catenin pathway axis[J]. Pathol Res Pract. 2022, 229:153727-153738.
19
Okoye I, Xu L, Motamedi M, et al. Galectin-9 expression defines exhausted T cells and impaired cytotoxic NK cells in patients with virus-associated solid tumors[J]. J Immunother Cancer. 2020, 8(2):e001849-e001861.
20
Peng Y, Qiu B, Tan F, et al. TIGIT/CD47 dual high expression predicts prognosis and is associated with immunotherapy response in lung squamous cell carcinoma[J]. Thorac Cancer. 2022, 13(14):2014-2023.
[1] 李康, 冀亮, 赵维, 林乐岷. 自噬在乳腺癌生物学进展中的双重作用[J]. 中华乳腺病杂志(电子版), 2023, 17(04): 195-202.
[2] 傅子财, 戴冠东, 朱伟民, 陆伟, 熊建义, 王大平, 邓桢翰. 过氧化物酶体增殖物激活受体在骨关节炎中的研究进展[J]. 中华关节外科杂志(电子版), 2023, 17(03): 363-367.
[3] 孔莹莹, 谢璐涛, 卢晓驰, 徐杰丰, 周光居, 张茂. 丁酸钠对猪心脏骤停复苏后心脑损伤的保护作用及机制研究[J]. 中华危重症医学杂志(电子版), 2023, 16(05): 355-362.
[4] 张晓燕, 肖东琼, 高沪, 陈琳, 唐发娟, 李熙鸿. 转录因子12过表达对脓毒症相关性脑病大鼠大脑皮质的保护作用及其机制[J]. 中华妇幼临床医学杂志(电子版), 2023, 19(05): 540-549.
[5] 刘星辰, 刘娟, 魏宝宝, 刘洁, 刘辉. XIAP与XAF1异常表达与卵巢癌的相关性分析[J]. 中华妇幼临床医学杂志(电子版), 2023, 19(04): 419-427.
[6] 江振剑, 蒋明, 黄大莉. TK1、Ki67蛋白在分化型甲状腺癌组织中的表达及预后价值研究[J]. 中华普外科手术学杂志(电子版), 2023, 17(06): 623-626.
[7] 刘硕儒, 王功炜, 张斌, 李书豪, 胡成. 新型溶瘤病毒M1激活内质网应激致前列腺癌细胞凋亡的机制[J]. 中华腔镜泌尿外科杂志(电子版), 2023, 17(04): 388-393.
[8] 郑嘉裕, 吴建杰, 李小娟, 曾恒达, 李国邦, 黄炯煅, 温星桥. hsa_circ_0090923在前列腺癌中的表达及其对前列腺癌细胞增殖和迁移的调控[J]. 中华腔镜泌尿外科杂志(电子版), 2023, 17(03): 276-283.
[9] 莫钊鸿, 翟航, 苏日顺, 孟泓宇, 罗豪, 陈文豪, 许瑞云. U2AF2表达对肝细胞癌增殖和迁移的影响及其与预后的关系[J]. 中华肝脏外科手术学电子杂志, 2023, 12(03): 336-341.
[10] 于迪, 于海波, 吴焕成, 李玉明, 苏彬, 陈馨. 发状分裂相关增强子1差异表达对胆固醇刺激下血管内皮细胞的影响[J]. 中华脑科疾病与康复杂志(电子版), 2023, 13(05): 264-270.
[11] 邓世栋, 刘凌志, 郭大勇, 王超, 黄忠欣, 张晖辉. 沉默SNHG1基因对膀胱癌细胞增殖、凋亡、迁移和铁死亡的影响[J]. 中华临床医师杂志(电子版), 2023, 17(07): 804-811.
[12] 张敏洁, 张小杉, 段莎莎, 施依璐, 赵捷, 白天昊, 王雅晳. 氢气治疗心肌缺血再灌注损伤的作用机制及展望[J]. 中华临床医师杂志(电子版), 2023, 17(06): 744-748.
[13] 方辉, 李菲, 张帆, 魏强, 陈强谱. 外源性瘦素对梗阻性黄疸大鼠肠黏膜增殖的影响[J]. 中华临床医师杂志(电子版), 2023, 17(05): 575-580.
[14] 郭如烨, 孟黎明, 陈楠, 宋玉莹, 尹海燕, 郭岩. Apelin/APJ系统对帕金森病模型的神经保护作用及机制研究进展[J]. 中华诊断学电子杂志, 2023, 11(04): 276-282.
[15] 邱甜, 杨苗娟, 胡波, 郭毅, 何奕涛. 亚低温治疗脑梗死机制的研究进展[J]. 中华脑血管病杂志(电子版), 2023, 17(05): 518-521.
阅读次数
全文


摘要

版权所有 中华医学会 中华医学电子音像出版社有限责任公司  京ICP备14006079号-1  网络出版服务许可证：(署)网出证(京)字第075号