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中华细胞与干细胞杂志(电子版) ›› 2019, Vol. 09 ›› Issue (01) : 7 -12. doi: 10.3877/cma.j.issn.2095-1221.2019.01.002

所属专题: 文献

论著

常见毛囊细胞角蛋白在毛囊周期中的表达研究
燕荣帅1, 裴卓2, 马小艮3, 张一鸣1, 李玉红2,()   
  1. 1. 400037 重庆,陆军军医大学新桥医院整形美容科
    2. 400038 重庆,陆军军医大学基础医学院细胞生物学教研室
    3. 400037 重庆,陆军军医大学新桥医院整形美容科;400038 重庆,陆军军医大学基础医学院细胞生物学教研室
  • 收稿日期:2018-12-12 出版日期:2019-02-01
  • 通信作者: 李玉红
  • 基金资助:
    国家自然科学基金面上项目(81472895); 重庆市自然科学基金项目(cstc2018jcyjAX0053)

Expression patterns of common hair follicle keratins in hair cycle

Rongshuai Yan1, Zhuo Pei2, Xiaogen Ma3, Yiming Zhang1, Yuhong Li2,()   

  1. 1. Department of Plastic and Cosmetic Surgery, Xinqiao Hospital, Army Medical University, Chongqing 400037, China
    2. Department of Cell Biology, Army Medical University, Chongqing 400038, China
    3. Department of Plastic and Cosmetic Surgery, Xinqiao Hospital, Army Medical University, Chongqing 400037, China; Department of Cell Biology, Army Medical University, Chongqing 400038, China
  • Received:2018-12-12 Published:2019-02-01
  • Corresponding author: Yuhong Li
  • About author:
    Corresponding author: Li Yuhong, Email:
引用本文:

燕荣帅, 裴卓, 马小艮, 张一鸣, 李玉红. 常见毛囊细胞角蛋白在毛囊周期中的表达研究[J/OL]. 中华细胞与干细胞杂志(电子版), 2019, 09(01): 7-12.

Rongshuai Yan, Zhuo Pei, Xiaogen Ma, Yiming Zhang, Yuhong Li. Expression patterns of common hair follicle keratins in hair cycle[J/OL]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2019, 09(01): 7-12.

目的

探讨常见毛囊细胞角蛋白在毛囊周期中的表达特征。

方法

取毛囊发育期、生长期启动、生长期、退化期和静止期的小鼠皮肤,石蜡切片后通过免疫荧光的方法,检测细胞角蛋白Krt5、Krt6、Krt10、Krt14、Krt15和Krt19的表达情况。

结果

Krt5在静止期和生长期启动表达于所有毛囊上皮细胞,在其他时期表达不一致;Krt6表达于所有时期的外根鞘细胞和内根鞘细胞;Krt10表达于生长期和退化期的毛母质和内根鞘细胞,在其他时期表达不一致;Krt14在生长期和退化期表达于所有毛囊上皮细胞,在其他时期表达不一致;Krt15和Krt19表达于毛囊发育期、生长期启动和静止期的毛囊隆突区细胞,在生长期和退化期表达不一致。

结论

角蛋白作为毛囊结构或毛囊干细胞标记物仅适用于特定的毛囊周期。研究者在使用毛囊角蛋白作为标记物时,应首先明确其在毛囊周期中的表达情况。

Objective

To evaluate the expression patterns of common hair follicle keratins in the hair follicle stages.

Methods

The mouse skins at different hair follicle stages including hair follicle morphogenesis, anagen onset, anagen, catagen, and telogen were harvested and embedded in paraffin. The sections were stained with anti-Krt5, anti-Krt6, anti-Krt10, anti-Krt14, anti-Krt15, and anti-Krt19 by immunostaining.

Results

Krt5 was expressed in the hair follicle epithelial of telogen and anagen stages. Krt6 was expressed in the outer root sheath and inner root sheath cells of hair follicles in all stages. Krt10 was expressed in the matrix and inner root sheath of anagen and catagen stages. Krt14 was expressed in the hair follicle epithelial of anagen and telogen stages. Krt15 and Krt19 were expressed in the bulge cells of morphogenesis, anagen onset, and telogen stages.

Conclusion

The keratins can only be used as hair structure or hair follicle stem cell markers at specific hair follicle stages. Researchers should determine the expression pattern of the hair follicle keratins in all hair follicle stages before using them as potential markers.

图1 正置荧光显微镜下观察Krt5在毛囊周期中的表达(×400)
图2 正置荧光显微镜下观察Krt6在毛囊周期中的表达(×400)
图3 正置荧光显微镜下观察Krt10在毛囊周期中的表达(×400)
图4 正置荧光显微镜下观察Krt14在毛囊周期中的表达(×400)
图5 正置荧光显微镜下观察Krt15在毛囊周期中的表达(×400)
图6 正置荧光显微镜下观察Krt19在毛囊周期中的表达(×400)
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