To evaluate the effect of rs37973 polymorphism on the promoter activity of GLCCI1 gene in Raji and THP-1 cells induced by fluticasone luciferase method.

The promoter of the human GLCCI1 gene was predicted by bioinformatics and obtained, and the luciferase vectors corresponding to rs37973-G and rs37973-A were constructed and then transfected into the cells. After treatment with 1 nmol/L fluticasone for 24 h, the luciferase activity in the cells of different treatment groups was detected. One-way ANOVA was used for comparison among multiple groups, and LSD test was used for comparison between groups.

The promoter of the human GLCCI1 gene was successfully obtained, and the luciferase expression vectors pGL3-pro (rs37973-G) and pGL3 pro (rs37973-A) were constructed. 48 h after transfection, compared with the control group, the luciferase activity in Raji and THP-1 cells transfected with reporter vector was statistically significant higher (23.82±2.79 vs 18.03±2.28, 24.52±2.62 vs 19.11±2.37) (P < 0.05) . Compared with pGL3-pro- (rs37973-G) -GLCCI1 transfection group, the luciferase activity in theRaji and THP- 1 cells treated with 1 nmol/L fluticasone for 24 h was significantly increased (30.05±5.23 vs 18.03± 2.28, 31.12±4.69 vs 19.11±2.37) (P < 0.05) . Compared with pGL3-pro (rs37973-A) -GLCCI1 transfection group, the luciferase activity in the Raji and THP-1 cells treated with 1 nmol/ L fluticasone for 24 hours was significantly increased (50.03±7.28 vs 23.82±2.79, 48.25±5.91 vs 24.52± 2.62) (P < 0.05) . Compared with the group transfected with pGL3-pro (rs37973-G) -GLCCI1 and treated with 1 nmol/L fluticasone for 24 h, the luciferase activity in Raji and THP-1 cells transfected with pGL3- pro (rs37973-A) -GLCCI1 and treated with 1 nmol/L fluticasone for 24 h also significantly increased (50.03±7.28 vs 31.02±5.23, 48.25±5.91 vs 31.12±4.69) (P < 0.05) .

37973 mutation can affect the promoter activity of GLCCI1, and rs37973-G can significantly reduce activity of GLCCI1 promoter in Raji and THP-1 cells induced by fluticasone.