To investigate the effects of miR-199a-5p targeting cell division cycle-associated 7-like protein (CDCA7L) on the migration and invasion of glioma cells.

Real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression of miR-199a-5p in human normal astrocyte HA1800 and human glioma cell line U251, U251 cells were transfected with Lipofectamine 2000 transfection kit and named blank group (normally cultured U251 cells) , miR-NC group (transfected with miR-NC) , miR-199a-5p mimic group (transfected with miR-199a-5p mimic) , miR-199a-5p mimic+pcDNA group (co-transfection of miR-199a-5p mimic and pcDNA) and miR-199a-5p mimic+pcDNA-CDCA7L group (co-transfection of miR-199a-5p mimic and pcDNA-CDCA7L) . Transwell experiment was used to detect the migration and invasion abilities of U251 cells. Western blot was used to detect the CDCA7L protein expression. TargetScan software was used to predict the binding sites of miR-199a-5p and CDCA7L, and dual luciferase reporter gene was used to verify the targeting relationship between miR-199a-5p and CDCA7L. The comparison between the HA1800 and U251 groups was performed by two-sample t test, the comparison among multiple groups was performed by one-way analysis of variance, and the comparison between the two groups was performed by SNK-q test.

Compared with HA1800 cells, the expression level of miR-199a-5p in U251 cells was decreased (1.09±0.26 vs 0.32 ± 0.14) , while the expression of CDCA7L protein in was increased (0.21±0.02 vs 0.85±0.13) , and the difference was statistically significant (P < 0.001) . The number of cell migration, invasion and CDCA7L protein expression in miR-199a- 5p mimic group were significantly lower than those in blank group and miR-NC group (50.12±3.36 vs 94.57±7.14, 93.86±6.11) (42.53±3.34 vs 71.49±4.35, 73.76±4.21) (0.25±0.07 vs 0.83±0.12, 0.89±0.09) , and the difference was statistically significant (P < 0.001) . CDCA7L was a target gene of miR-199a-5p. Overexpression of CDCA7L could reverse the inhibitory effects of up-regulating miR-199a-5p on the migration and invasion of U251 cells (P < 0.05) .

The expression level of miR-199a-5p is low in glioma cells, and up-regulation of miR- 199a-5p may inhibit the migration and invasion of glioma cells by targeting inhibition of CDCA7L protein expression.