Characteristics and clinical significance of peripheral blood N-alpha-acetyltransferase 10 and lymphocyte immunophenotyping in patients with oral squamous cell carcinoma

doi:10.3877/cma.j.issn.2095-1221.2019.04.001

Abstract

Abstract:

Objective

To analyze the characteristics of peripheral blood N-alpha-acetyltransferase 10 (Naa10) and lymphocyte immunophenotyping in patients with oral squamous cell carcinoma (OSCC) and to explore its clinical significance.

Methods

97 OSCC patients and 50 health examinees were enrolled in the patient group and the control group respectively. Naa10 and lymphocyte immunophenotyping in the peripheral blood of the two groups were detected. The differences of Naa10 and lymphocyte immunophenotyping in the peripheral blood of patients with different TNM stages and lymph node metastasis status were compared, and their characteristics and clinical significance were summarized.

Results

The serum Naa10 in the patients group was (62.91±17.15) pg/ml, which was higher than that in the control group (38.82±9.04) pg/ml (t?= 9.280, P?< 0.05) . The percentages of CD3⁺, CD4⁺, CD4⁺/CD8⁺, NK cells in the patients group were (71.63±9.11) ﹪, (34.34±4.26) ﹪, (1.03±0.28) and (14.23±4.61) ﹪, which were lower than those in the control group (76.25±2.87) ﹪, (45.41±7.08) ﹪, (1.77±0.39) and (19.96±5.13) ﹪. The percentages of CD8⁺, B lymphocyte in the patients group were (14.23±4.61) ﹪, (14.91±3.29) ﹪, which were higher than those in the latter group (19.96±5.13) ﹪, (9.11±2.60) ﹪. The serum Naa10 of 42 patients with TNM stageⅠ~Ⅱwas (64.08±15.26) pg/mL. There was no significant difference between the serum Naa10 and the serum Naa10 of patients with TNM stage Ⅲ~Ⅳ (61.71±13.15) pg/ml (t?= 0.820, P?> 0.05) . There were 34 cases with lymph node metastasis, and the serum Naa10 was (65.15 ±14.07) ?pg/ml. There was no significant difference between those without lymph node metastasis and those without lymph node metastasis (61.45±12.66) pg/ml (t?= 1.321, P?> 0.05) . The percentages of CD3⁺, CD4⁺, CD4⁺/CD8⁺, NK cells in stageⅠ~Ⅱ patients were lower than those in stage Ⅲ~Ⅳ patients, and the percentages of CD8⁺, B lymphocyte in stageⅠ~Ⅱpatients were higher than those in stage Ⅲ~Ⅳ patients (P?< 0.05) . Pearson correlation analysis showed that Naa10 in peripheral blood was negatively correlated with CD3⁺ (-0.631) , CD4⁺ (-0.529) , CD4⁺/CD8⁺ (-0.587) , percentage of NK cells (-0.603) , and positively correlated with CD8⁺ (0.558) and percentage of B lymphocyte (0.670) (P?< 0.05) .

Conclusion

Naa10 in peripheral blood of OSCC patients was increased significantly, but not related to TNM stage and lymph node metastasis. The lymphocyte immunophenotype of OSCC patients changed significantly, and the increase of TNM stage and lymph node metastasis was accompanied by the decrease of CD3⁺, CD4⁺, CD4⁺/CD8⁺, NK cell percentage and the increase of CD8⁺, B lymphocyte percentage.

Key words: Oral squamous cell carcinoma, N-alpha-acetyltransferase 10, Lymphocyte, Immunophenotyping

Yi Zeng, Congji Zhang, Yanyan Ye, Junfeng Guo, Jun Yang, Yu Xiong. Characteristics and clinical significance of peripheral blood N-alpha-acetyltransferase 10 and lymphocyte immunophenotyping in patients with oral squamous cell carcinoma[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2019, 09(04): 193-198.

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References 18

1	Nguyen N, Bellile E, Thomas D, et al. Tumor infiltrating lymphocytes and survival in patients with head and neck squamous cell carcinoma[J]. Head Neck, 2016, 38(7):1074-1084.
2	马超, 赵继志, 张韬, 等. 细胞黏附分子-1和淋巴细胞功能相关抗原在口腔鳞癌中的表达及其临床意义[J]. 中国药物与临床, 2015, 15(9):1337-1339.
3	Nakashima H, Matsuoka Y, Yoshida R, et al. Pre-treatment neutrophil to lymphocyte ratio predicts the chemoradiotherapy outcome and survival in patients with oral squamous cell carcinoma: a retrospective study[J]. BMC Cancer, 2016, 16:41.
4	李怡宁, 陈思远, 胡济安. O-糖基化在口腔鳞状细胞癌发生发展中的作用初探[J]. 口腔生物医学, 2015, 6(4):172-177.
5	Chen S, Guo JB, Feng CJ, et al. The preoperative platelet-lymphocyte ratio versus neutrophil-lymphocyte ratio: which is better as a prognostic factor in oral squamous cell carcinoma?[J]. Ther Adv Med Oncol, 2016, 8(3):160-167.
6	Wolf GT, Chepeha DB, Bellile E, et al. Tumor infiltrating lymphocytes (TIL) and prognosis in oral cavity squamous carcinoma: A preliminary study[J]. Oral Oncol, 2015, 51(1):90-95.
7	Straub M, Drecoll E, Pfarr N, et al. CD274/PD-L1 gene amplification and PD-L1 protein expression are common events in squamous cell carcinoma of the oral cavity[J]. Oncotarget, 2016, 7(11):12024-12034.
8	余芯乐, 郑军, 徐江, 等. 口腔鳞状细胞癌患者唾液和血清中N-α-乙酰基转移酶10的水平及其临床意义[J]. 国际口腔医学杂志, 2018, 45(4):391-395.
9	Wirsing AM, Rikardsen OG, Steigen SE, et al. Presence of tumour high-endothelial venules is an Independent positive prognostic factor and stratifies patients with advanced-stage oral squamous cell carcinoma[J]. Tumor Biol, 2016, 37(2):2449-2459.
10	孙晓静. 口腔鳞状细胞癌患者治疗前后外周血免疫细胞的检测及其临床意义的研究[D]. 郑州: 郑州大学, 2017.
11	Wirsing AM, Ervik IK, Seppola M, et al. Presence of high-endothelial venules correlates with a favorable immune microenvironment in oral squamous cell carcinoma[J]. Mod Pathol, 2018, 31(6):910-922.
12	Rassouli A, Saliba J, Castano R, et al. Systemic inflammatory markers as Independent prognosticators of head and neck squamous cell carcinoma[J]. Head Neck, 2015, 37(1):103-110.
13	郑军, 徐江, 余芯乐, 等. N-乙酰基转移酶10蛋白和朊蛋白在口腔鳞状细胞癌组织中的表达及相关性研究[J]. 口腔医学研究, 2016, 32(5):483-486.
14	Satgunaseelan L, Gupta R, Madore J, et al. Programmed cell death-ligand 1 expression in oral squamous cell carcinoma is associated with an inflammatory phenotype[J]. Pathology, 2016, 48(6):574-580.
15	杨柳, 陈娟, 单忠艳, 等. 口腔鳞状细胞癌患者外周血淋巴细胞免疫分型及临床意义[J]. 口腔医学研究, 2018, 34(5):93-98.
16	Groeger S, Howaldt HP, Raifer H, et al. Oral squamous carcinoma cells Express B7-H1 and B7-DC receptors in vivo[J]. Pathol Oncol Res, 2017, 23(1):99-110.
17	Farhan-Alanie OM. McMahon J, McMillan DC. Systemic inflammatory response and survival in patients undergoing curative resection of oral squamous cell carcinoma[J]. Br J Oral Maxillofacl Surg, 2015, 53(2):126-131.
18	Kogashiwa Y, Yasuda M, Sakurai HA, et al. PD-L1 expression confers better prognosis in locally advanced oral squamous cell carcinoma[J]. Anticancer Res, 2017, 37(3):1417-1424.

分组	例数	CD3⁺ （﹪）	CD4⁺ （﹪）	CD8⁺ （﹪）	CD4⁺/ CD8⁺	NK细胞（﹪）	B淋巴细胞（﹪）
患者组	97	71.63±9.11	34.31±4.26	33.19±4.52	1.03±0.28	14.23±4.61	14.91±3.29
对照组	50	76.25±2.87	45.41±7.08	25.61±4.03	1.77±0.39	19.96±5.13	9.11±2.60
t值	?	3.493	11.849	9.985	13.225	6.868	10.837
P值	?	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05

分组	例数	CD3⁺ （﹪）	CD4⁺ （﹪）	CD8⁺ （﹪）	CD4⁺/ CD8⁺	NK细胞（﹪）	B淋巴细胞（﹪）
患者组	97	71.63±9.11	34.31±4.26	33.19±4.52	1.03±0.28	14.23±4.61	14.91±3.29
对照组	50	76.25±2.87	45.41±7.08	25.61±4.03	1.77±0.39	19.96±5.13	9.11±2.60
t值	?	3.493	11.849	9.985	13.225	6.868	10.837
P值	?	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05

TNM分期	例数	CD3⁺ （﹪）	CD4⁺ （﹪）	CD8⁺ （﹪）	CD4⁺/ CD8⁺	NK细胞（﹪）	B淋巴细胞（﹪）
Ⅰ~Ⅱ期	42	74.17±6.42	37.08±3.90	30.32±4.11	1.22±0.17	16.09±2.29	13.35±1.74
Ⅲ~Ⅳ期	55	69.69±5.71	32.19±2.80	35.38±3.34	0.88±0.16	12.81±1.88	16.10±2.29
t值	?	3.628	7.188	6.688	10.093	7.744	6.481
P值	?	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05

TNM分期	例数	CD3⁺ （﹪）	CD4⁺ （﹪）	CD8⁺ （﹪）	CD4⁺/ CD8⁺	NK细胞（﹪）	B淋巴细胞（﹪）
Ⅰ~Ⅱ期	42	74.17±6.42	37.08±3.90	30.32±4.11	1.22±0.17	16.09±2.29	13.35±1.74
Ⅲ~Ⅳ期	55	69.69±5.71	32.19±2.80	35.38±3.34	0.88±0.16	12.81±1.88	16.10±2.29
t值	?	3.628	7.188	6.688	10.093	7.744	6.481
P值	?	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05

淋巴结转移	例数	CD3⁺ （﹪）	CD4⁺ （﹪）	CD8⁺ （﹪）	CD4⁺/ CD8⁺	NK细胞（﹪）	B淋巴细胞（﹪）
有	34	63.50±6.62	30.31±3.55	37.39±3.52	0.81±0.15	12.18±2.97	16.72±2.50
无	63	76.02±5.37	36.47±2.98	30.92±3.10	1.15±0.19	15.34±3.31	13.93±2.68
t值	?	10.084	9.076	9.349	9.02	4.646	5.006
P值	?	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05

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