To explore whether exosomal miRNA-1246 promotes proliferation and invasion acts of astroglioma cells.

Exosomes were isolated from the patient's blood and their components were identified. The changes in the invasiveness of astroglioma cells under the action of microrna-1246 were examined by transwell chamber test. Luciferase reporter assay was used to verify whether cell adhesion molecule 1 (CADM1) is the target gene of miRNA-1246. Finally, Western Blot and RT-qPCR were used to detect CADM1 protein in cancer tissues and analyzed its relationship with glioma. ANOVA and t test were used for statistical analysis.

The contents of microRNA-1246 in the exosomes of blood circulation in patients with malignant glioma (2.83±1.70) were significantly increased compared with control group (1.00±0.50) (P?<?0.05). Cell invasion in the miRNA-1246 inhibitor group (48.40±5.90) decreased compared with the control group (96.50±6.70). In the luciferase reporter gene assay, fluorescence intensity of the miRNA-1246 mimic group (4.98±1.86) significantly decreased compared with the control group (10.34±2.60) (P?<?0.05). In vitro experiments showed that microRNA-1246 can affect the expression of CADM1 gene, and thereby affect the proliferation and migration of glioma cells.

MicroRNA-1246 in glioma cell derived exosomes can promote the proliferation and metastasis of glioma cells by inhibiting CADM1 expression, indicating that microRNA-1246 can be used as a potential target for the diagnosis and treatment of malignant glioma.