Advances in co-culture promoted neuralization strategy in tissue engineering bone construction

doi:10.3877/cma.j.issn.2095-1221.2022.05.006

Abstract

Abstract:

Tissue engineering bone transplantation is an important method for the treatment of large-segment bone defects. Co-cultivation of selected tissues or cells in a specific culture mode can make tissue engineering bone more neural, which can effectively enhance the repair effect of tissue engineering bone on the damaged bone tissue. The literature related to co-culture promoting neuralization strategy in tissue engineered bone construction was reviewed from three aspects inthisarticle: tissue and seed cell selection, the molecular mechanism of tissue and seed cell interaction, and application of co-culture mode, in order to provide a reference for the further development of the technology and its clinical applications.

Key words: Co-culture, Neuralization, Tissue engineering bone

Shiwen Lin, Hui Sun, Nana Chen, Cong Zhu. Advances in co-culture promoted neuralization strategy in tissue engineering bone construction[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2022, 12(05): 293-299.

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References 67

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共培养组织/细胞	体外检测	体内检测	优势	参考文献
大隐神经/组织工程骨	n.a	NPY	大隐神经支配的组织工程骨具有更佳的促进骨修复能力	覃俊君等^[3]
SCs/自体颅骨组织	骨钙素、骨源性碱性磷酸酶、骨桥蛋白	n.a	SCs与骨组织共培养加强成骨细胞的骨修复能力	李钟鹏等^[4]
BMSCs/壳聚糖多孔支架	n.a	溴脱氧核苷尿嘧啶、胶质纤维酸性蛋白	壳聚糖多孔支架与BMSCs共培养能明显改善大鼠受损的神经功能	谭可可等^[5]
多能干细胞/神经前体细胞	巢蛋白	n.a	多能干细胞与神经前体细胞共培养可诱导未分化人类多能干细胞的神经转化	Rhee等^[6]
SCs/MSCs	磷酸化蛋白激酶B、B淋巴细胞瘤-2蛋白	n.a	SCs与MSCs共培养可抑制SCs的凋亡，对神经病变起修复作用	Zhang等^[7]
BMSCs /新生儿海马细胞	n.a	巢蛋白、微管蛋白	BMSCs与海马细胞共培养促进BMSCs向神经元分化	Majeed等^[8]
隐神经/组织工程骨	n.a	组织工程骨形成	植入隐神经的组织工程骨具有更佳的促进骨缺损修复能力	江汕等^[12]
骨皮质/骨髓	BMP2、TGF-β1	骨折愈合情况	骨皮质与骨髓共培养的MSCs具有更好的促进骨折愈合能力	Zhu等^[18]
SCs/ADSCs	神经元特异性烯醇化酶、微管关联蛋白2、胶质纤维酸性蛋白、神经元特异性核蛋白	n.a	SCs与ADSCs共培养可显著促进ADSCs向神经元样细胞分化	张振辉等^[19]
SCs/同种异体骨	S-100蛋白	n.a	SCs与支架具有良好的组织相融性	周翔等^[20]
骨膜细胞/髓核细胞	骨钙素、I型胶原、RUNX2 、ALP	n.a	骨膜细胞与髓核细胞共培养在低氧环境里成骨分化能力更强	陆海涛等^[50]

共培养组织/细胞	体外检测	体内检测	优势	参考文献
大隐神经/组织工程骨	n.a	NPY	大隐神经支配的组织工程骨具有更佳的促进骨修复能力	覃俊君等^[3]
SCs/自体颅骨组织	骨钙素、骨源性碱性磷酸酶、骨桥蛋白	n.a	SCs与骨组织共培养加强成骨细胞的骨修复能力	李钟鹏等^[4]
BMSCs/壳聚糖多孔支架	n.a	溴脱氧核苷尿嘧啶、胶质纤维酸性蛋白	壳聚糖多孔支架与BMSCs共培养能明显改善大鼠受损的神经功能	谭可可等^[5]
多能干细胞/神经前体细胞	巢蛋白	n.a	多能干细胞与神经前体细胞共培养可诱导未分化人类多能干细胞的神经转化	Rhee等^[6]
SCs/MSCs	磷酸化蛋白激酶B、B淋巴细胞瘤-2蛋白	n.a	SCs与MSCs共培养可抑制SCs的凋亡，对神经病变起修复作用	Zhang等^[7]
BMSCs /新生儿海马细胞	n.a	巢蛋白、微管蛋白	BMSCs与海马细胞共培养促进BMSCs向神经元分化	Majeed等^[8]
隐神经/组织工程骨	n.a	组织工程骨形成	植入隐神经的组织工程骨具有更佳的促进骨缺损修复能力	江汕等^[12]
骨皮质/骨髓	BMP2、TGF-β1	骨折愈合情况	骨皮质与骨髓共培养的MSCs具有更好的促进骨折愈合能力	Zhu等^[18]
SCs/ADSCs	神经元特异性烯醇化酶、微管关联蛋白2、胶质纤维酸性蛋白、神经元特异性核蛋白	n.a	SCs与ADSCs共培养可显著促进ADSCs向神经元样细胞分化	张振辉等^[19]
SCs/同种异体骨	S-100蛋白	n.a	SCs与支架具有良好的组织相融性	周翔等^[20]
骨膜细胞/髓核细胞	骨钙素、I型胶原、RUNX2 、ALP	n.a	骨膜细胞与髓核细胞共培养在低氧环境里成骨分化能力更强	陆海涛等^[50]

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