To investigate the effect of LncRNA AC130710 on proliferation, apoptosis and epithelial mesenchymal transformation (EMT) of endometrial cancer cells (HEC-1A cells) through miR-129-5p/WNT4 axis and its mechanism.

The expressions of LncRNA AC130710, miR-129-5P and WNT4 in HEC-1A and HEEC cells were detected by real-time quantitative PCR (RT-qPCR) , then the cells were transfected with (1) siRNA NC, AC130710 siRNA and WNT4 siRNA; (2) inhibitor NC, miR-129-5p inhibitor; (3) pcDNA-3.1 (+) + mimics NC, pcDNA-AC130710+ mimics NC, pcDNA-3.1 (+) +miR-129-5p mimics, pcDNA-AC130710+ miR-129-5p mimics. The effects of LncRNA AC130710, miR-129-5P and WNT4 expressions on the proliferation ability of HEC-1A cells were detected by MTT experiment. Western blot was used to detect the effects of LncRNA AC130710, miR-129-5p and WNT4 on the expression of apoptosis-related proteins Bax,cleaved-caspase-3, cleaved-caspase-9 and Bcl-2 in HEC-1A cells. Western blot assay was used to detect the effects of LncRNA AC130710, miR-129-5P and WNT4 expressions on EMT of HEC-1A cells. The targeted relationship between LncRNA AC130710 and miR-129-5P was analyzed by miRanda and double luciferase reporter gene assay. The correlation between miR-129-5P and WNT4 was analyzed by the TargetScan database, and the interaction between miR-129-5P and WNT4 was detected by the double luciferase reporter gene. RT-qPCR detected the effect of LncRNA AC130710 on WNT4 expression through miR-129-5P.

Compared with HEEC cells, AC130710 expression was increased in HEC-1A cells (1.86±0.21 vs 0.85±0.06) . The expression level of miR-129-5p in HEC-1A cells was decreased (0.89±0.16 vs 1.76±0.08) ; Compared with HEEC cells, the expression of WNT4 in HEC-1A cells was increased (1.88±0.26 vs 1.08±0.12) . Compared with the siRNA NC transfection group, the relative expression levels of Bax (1.37±0.14 vs 0.84±0.21) , cleaved caspase-3 (1.08±0.16 vs 0.37±0.07) , cleaved caspase-9 (1.26±0.24 vs 0.39±0.06) , E-cadherin (1.87±0.17 vs 1.32±0.26) in AC130710 siRNA transfection group increased, the relative expression levels of Bcl-2 (0.38±0.08 vs 1.18±0.14) , N-cadherin (0.36±0.04 vs 0.85±0.24) , Vimentin (0.35±0.09 vs 1.12±0.18) and Snail (0.42±0.10 vs 1.26±0.27) decreased. LncRNA AC130710 3'UTR has a direct targeting relationship with miR-129-5P. Compared with the inhibitor NC transfection group, the relative expression levels of Bcl-2 (0.98±0.07 vs 0.65±0.08) , N-cadherin (1.39±0.15 vs 0.68±0.09) , Vimentin (1.16±0.16 vs 0.54±0.02) and Snail (0.95±0.08 vs 0.42±0.06) protein increased, the relative expression levels of Bax (0.27±0.09 vs 0.85±0.13) , cleaved caspase-9 (0.52±0.14 vs 1.19±0.15) , cleaved caspase-3 (0.48±0.05 vs 1.16±0.28) and E-cadherin (0.43±0.09 vs 1.08±0.26) proteins decreased. LncRNA AC130710 overexpression promoted HEC-1A cell proliferation and EMT through miR-129-5P. miR-129-5P specifically binds to the WNT4 3'UTR. Compared with the siRNA NC transfected group, the proliferation ability of WNT4 siRNA relative expression level of Bcl-2 (0.23±0.08 vs 0.84±0.12) , N-cadherin (0.28±0.09 vs 1.14±0.17) , Vimentin (0.35±0.08 vs 1.13±0.08) and Snail (0.42±0.23 vs 1.06±0.15) protein decreased. The relative expression level of Bax (0.96±0.12 vs 0.42±0.08) , cleaved caspase-3 (1.13±0.25 vs 0.45±0.06) , cleaved caspase-9 (1.54±0.23 vs 0.72±0.12) , and E-cadherin (1.87±0.24 vs 1.26±0.18) increased. Downregulation of LncRNA AC130710 inhibits WNT4 expression via miR-129-5P.

LncRNA AC130710 may regulate the proliferation, apoptosis and EMT of endometrial cancer cells (HEC-1A cells) by miR-129-5P/WNT4 axis.