Mechanisms of polysaccharides of radix platycodonis in the proliferation and apoptosis of liver cancer cells via LINC01554

doi:10.3877/cma.j.issn.2095-1221.2022.01.003

Abstract

Abstract:

Objective

To explore the effects and mechanisms of polysaccharides of radix platycodonis on the proliferation and apoptosis of liver cancer cells.

Methods

Liver cancer cells Huh-7 were treated with low, medium and high dose (20, 40, 60 μg/mL) polysaccharides of radix platycodonis respectively. DMSO-treated Huh-7 cells were served as control. In addition, pcDNA and pcDNA-LINC01554 were transfected into Huh-7 cells. si-NC and si-LINC01554 were transfected into Huh-7 cells respectively and then treated with polysaccharides of radix. Cell proliferation and clone formation ability were detected by MTT experiment and plate clone formation experiment. Apoptosis rate was detected by Flow cytometry. The expression of LINC01554 was detected by RT-qPCR. The comparison between the two groups of data was performed by t test, and the comparison of multiple groups was performed by one-way analysis of variance.

Results

Compared with the control, the cell viability (1.26±0.09 vs 1.05±0.06, 0.77±0.05, 0.47± 0.03) and the number of colonies formed (117.00±4.55 vs 99.67±3.86, 71.67±2.87, 54.67±2.05) were decreased in low, medium and high dose polysaccharides of radix platycodonis treated cells in a dose-dependent manner, while the apoptosis rate [ (8.24±0.49) ﹪ vs (13.47±0.72) ﹪, (18.20±0.84) ﹪, 22.52±1.12) ﹪] and the expression level of LINC01554 (1.00±0.00 vs 1.35±0.05, 1.90±0.06, 3.38±0.09) were increased significantly in a dose-dependent manner (all P < 0.05) . Compared with the pcDNA, cell viability (1.24±0.08 vs 0.56±0.03) and the colony formation number (116.67±4.19 vs 64.33±2.49) were decreased in pcDNA-LINC01554 transfected cells, while the apoptosis rate was increased [ (8.13±0.60) ﹪vs (20.45±0.97) ﹪] (all P < 0.05) . Compared with the polysaccharides of radix platycodonis+si-NC, the cell viability (0.48±0.03 vs 1.12±0.06) and the number of colonies formed (55.00±2.16 vs 107.67±3.40) were increased in the polysaccharides of radix platycodonis+si-LINC01554 treated cells, while the apoptosis rate was decreased [ (22.51±1.10) ﹪vs 10.52±0.49) ﹪] ( all P < 0.05) .

Conclusion

Polysaccharides of radix platycodonis could reduce the proliferation and cloning ability of liver cancer cells and induce apoptosis by up-regulating the expression of LINC01554.

Key words: Polysaccharides of radix platycodonis, Long noncoding RNA, Liver cancer, Proliferation, Apoptosis

Yanwei Cai, Yijiong Yao. Mechanisms of polysaccharides of radix platycodonis in the proliferation and apoptosis of liver cancer cells via LINC01554[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2022, 12(01): 14-18.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhxbygxbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-1221.2022.01.003

https://zhxbygxbzz.cma-cmc.com.cn/EN/Y2022/V12/I01/14

Figures/Tables 9

References 13

1	周孟，廖祥明，王珊, 等. 槲皮素抑制人肝癌细胞HepG2的体内外活性研究[J]. 安徽医药, 2019, 23(11):2136-2141.
2	韩康，包永睿，王帅, 等. 中药地榆对肝癌HepG2细胞的抑制作用及机制研究[J]. 辽宁中医药大学学报, 2019, 21(5):30-32.
3	展凡，王玉涵，王萌, 等. 新藤黄酸抑制人肝癌细胞BEL-7402自噬作用研究[J]. 安徽中医药大学学报, 2020, 39(3):46-50.
4	He JQ, Zheng MX, Ying HZ, et al. PRP1, a heteropolysaccharide from platycodonis radix, induced apoptosis of HepG2 cells via regulating miR-21-mediated PI3K/AKT pathway[J]. Int J Biol Macromol, 2020, 158(1):542-551.
5	Zheng YL, Li L, Jia YX, et al. LINC01554-mediated glucose metabolism reprogramming suppresses tumorigenicity in hepatocellular carcinoma via downregulating PKM2 expression and inhibiting Akt/mTOR signaling pathway[J]. Theranostics, 2019, 9(3):796-810.
6	陆文总，杨亚丽，贾光锋, 等. 桔梗多糖对U-14宫颈癌抗肿瘤作用的研究[J]. 西北药学杂志, 2013, 28(1):43-45.
7	Sukowati CHC, Anfuso B, Fiore E, et al. Hyaluronic acid inhibition by 4-methylumbelliferone reduces the expression of cancer stem cells markers during hepatocarcinogenesis[J]. Sci Rep, 2019, 9(1):4026-4036.
8	Feng B, Zhu Y, Sun C, et al. Basil polysaccharide inhibits hypoxia-induced hepatocellular carcinoma metastasis and progression through suppression of HIF-1alpha-mediated epithelial-mesenchymal transition[J]. Int J Biol Macromol, 2019, 137(1):32-44.
9	顾程远，陈秋利，李海涛. 桔梗多糖对H2O2诱导PC12细胞氧化损伤的保护作用及机制研究[J]. 南京中医药大学学报, 2017, 33(3):268-272.
10	姚欢欢，陈吉，李岚. 桔梗多糖对哮喘小鼠气道炎症和气道重塑的影响[J]. 中国中医药科技, 2020, 27(5):701-707.
11	Dasgupta A, Dey D, Ghosh D, et al. Astrakurkurone, a sesquiterpenoid from wild edible mushroom, targets liver cancer cells by modulating Bcl-2 family proteins[J]. IUBMB Life, 2019, 71(7):992-1002.
12	Ding Y, Sun Z, Zhang S, et al. Down-regulation of long non-coding RNA LINC01554 in hepatocellular cancer and its clinical significance[J]. J Cancer, 2020, 11(11):3369-3374.
13	Li L, Huang K, Lu Z, et al. Bioinformatics analysis of LINC01554 and its co-expressed genes in hepatocellular carcinoma[J]. Oncol Rep, 2020, 44(5):2185-2197.

基因	序列（5'→3'）	预期产物扩增长度（bp）
LINC01554	上游GGAGGTCGGTTGATGAGCAGT	243
	下游GTCAAGCCTGTGTGTCATCGTT
GAPDH	上游CAGGAGGCATTGCTGATGAT	293
	下游GAAGGCTGGGGCTCATTT

基因	序列（5'→3'）	预期产物扩增长度（bp）
LINC01554	上游GGAGGTCGGTTGATGAGCAGT	243
	下游GTCAAGCCTGTGTGTCATCGTT
GAPDH	上游CAGGAGGCATTGCTGATGAT	293
	下游GAAGGCTGGGGCTCATTT

分组	实验次数	细胞活力	集落形成数（个）	凋亡率（﹪）	Bax	Bcl-2
对照	3	1.26±0.09	117.00±4.55	8.24±0.49	0.13±0.01	0.74±0.06
20 μg/mL桔梗多糖	3	1.05±0.06^a	99.67±3.86^a	13.47±0.72^a	0.27±0.02^a	0.53±0.05^a
40 μg/mL桔梗多糖	3	0.77±0.05^ab	71.67±2.87^ab	18.20±0.84^ab	0.44±0.04^ab	0.35±0.03^ab
60 μg/mL桔梗多糖	3	0.47±0.03^abc	54.67±2.05^abc	22.52±1.12^abc	0.68±0.06^abc	0.14±0.01^abc
F值		93.583	194.377	166.787	118.035	110.535
P值		< 0.001	< 0.001	< 0.001	< 0.001	< 0.001

分组	实验次数	细胞活力	集落形成数（个）	凋亡率（﹪）	Bax	Bcl-2
对照	3	1.26±0.09	117.00±4.55	8.24±0.49	0.13±0.01	0.74±0.06
20 μg/mL桔梗多糖	3	1.05±0.06^a	99.67±3.86^a	13.47±0.72^a	0.27±0.02^a	0.53±0.05^a
40 μg/mL桔梗多糖	3	0.77±0.05^ab	71.67±2.87^ab	18.20±0.84^ab	0.44±0.04^ab	0.35±0.03^ab
60 μg/mL桔梗多糖	3	0.47±0.03^abc	54.67±2.05^abc	22.52±1.12^abc	0.68±0.06^abc	0.14±0.01^abc
F值		93.583	194.377	166.787	118.035	110.535
P值		< 0.001	< 0.001	< 0.001	< 0.001	< 0.001

分组	实验次数	LINC01554
对照	3	1.00±0.00
20 μg/mL桔梗多糖	3	1.35±0.05^a
40 μg/mL桔梗多糖	3	1.90±0.06^ab
60 μg/mL桔梗多糖	3	3.38±0.09^abc
F值		930.331
P值		< 0.001

Please choose a citation manager

Content to export