Study on the changes of cellular immunity and humoral immunity in patients with cerebral infarction

doi:10.3877/cma.j.issn.2095-1221.2018.06.004

Abstract

Abstract:

Objective

To investigate the changes of cellular and humoral immune function in patients with cerebral infarction.

Methods

From May 2016 to May 2017, 120 patients with cerebral infarction admitted to the Central Hospital of Chancheng District were included and were divided into mild cerebral infarction group (middle cerebral artery infarction, the diameter of infarction area < 5 cm, n = 60) and medium and severe group (middle cerebral artery infarction, the diameter of infarction area > 5 cm, n = 60). 120 healthy persons were selected as healthy control group. Flow cytometry (FCM) and rate scattering immune transmission turbidimetric method were used to determine lgA, lgM and lgG, and the levels of complement C3 and C4 in each group. The levels of CD3⁺, CD4⁺, CD8⁺, NK cells, B cells and CD4⁺/CD8⁺ percentage in peripheral blood of patients with stroke were measured. The hemodynamic index and anticardiolipin antibody (ACA) concentration were measured. The biochemical changes in carotid atherosclerosis (CAS) reflected by ACA concentration were measured. One-way ANOVA was used for comparison between groups. The sex ratio of the patients was assayed with χ² test, the comparison between groups was chi-square analysis. The correlation between cellular immunity and humoral immunity was analyzed by linear correlation.

Results

The content of T lymphocyte in the severe cerebral infarction group was lower than that in the mild cerebral infarction group and the control group (P < 0.05). The percentage of CD8⁺ cells (27.91%±2.97%), the ratio of CD4⁺/CD8⁺ (1.98±0.03) were higher than that in the mild cerebral infarction group and the control group (P < 0.05). NK cells (12.29% ±1.58%) and B cells (12.76% ±2.00%) decreased in the severe group (P < 0.05). Compared with mild cerebral infarction group and control group, the serum levels of immunoglobulin IgG (10.60±1.06) IU/ ml decreased in the severe cerebral infarction group (P < 0.05), immunoglobulin IgA (4.01±0.35) IU/ ml, complement C3 (2.13±0.04) IU/ml and complement C4 (0.39±0.01) IU/ ml increased in severe cerebral infarction group (P < 0.05), but there was no significant difference in IgM (2.60±0.05) IU/ ml. Compared with mild cerebral infarction group (14.11±2.09) PLU/ml and control group (7.82±1.15) PLU/ml, serum ACA concentration in the severe cerebral infarction group (16.88±2.50) PLU/ml was increased (t = 9.153, P = 0.000). Compared with mild cerebral infarction group and control group, the contents of total cholesterol [(1.75±0.03) mmol/L] and triglyceride [(2.42±0.33) mmol/L] increased, the contents of high density lipoprotein cholesterol [(0.41±0.03) mmol/L] decreased, and the contents of low density lipoprotein cholesterol; (1.55 ±0.21) mmol/L) increased in moderate and severe cerebral infarction group (P < 0.05). The correlation analysis between cellular immune parameters and humoral immune parameters showed a negative correlation (P < 0.05).

Conclusion

The changes of cellular and humoral immunity in patients with cerebral infarction can guide the treatment and prognosis of cerebral infarction.

Key words: Cerebral infarction patients, Cellular immunity, Humoral immunity, Biochemical indices, Hemocyte immunoglobulin

Li Ren, Mingna Huang, Jiling Feng, Xihua Wu, Shiya Su. Study on the changes of cellular immunity and humoral immunity in patients with cerebral infarction[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2018, 08(06): 343-347.

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分组	例数	男/女	年龄（岁）
对照组	120	72/48	56.38±7.19
脑梗死轻症组	60	38/22	57.27±7.50
脑梗死中重症组	60	35/25	57.95±8.22
χ² / F值		0.665	0.922
P值		0.523	0.399

分组	例数	男/女	年龄（岁）
对照组	120	72/48	56.38±7.19
脑梗死轻症组	60	38/22	57.27±7.50
脑梗死中重症组	60	35/25	57.95±8.22
χ² / F值		0.665	0.922
P值		0.523	0.399

分组	例数	CD3⁺（%）	CD4⁺（%）	CD8⁺（%）	CD4⁺/CD8⁺	NK细胞（%）	B细胞（%）
对照组	120	56.33±8.21	39.81±4.10	21.66±5.17	0.84±0.11	27.45±2.13	19.71±3.16
脑梗死轻症组	60	40.03±4.16^a	23.20±3.52^a	24.75±2.15^a	1.21±0.02^a	15.81±1.12^a	15.79±1.57^a
脑梗死中重症组	60	45.22±5.53^ab	28.11±4.09^ab	27.91±2.97^ab	1.98±0.03^ab	12.29±1.58^ab	12.76±2.00^ab
F值		6.281	8.152	10.313	8.851	9.525	9.862
P值		0.005	0.002	0.001	0.000	0.000	0.000

分组	例数	CD3⁺（%）	CD4⁺（%）	CD8⁺（%）	CD4⁺/CD8⁺	NK细胞（%）	B细胞（%）
对照组	120	56.33±8.21	39.81±4.10	21.66±5.17	0.84±0.11	27.45±2.13	19.71±3.16
脑梗死轻症组	60	40.03±4.16^a	23.20±3.52^a	24.75±2.15^a	1.21±0.02^a	15.81±1.12^a	15.79±1.57^a
脑梗死中重症组	60	45.22±5.53^ab	28.11±4.09^ab	27.91±2.97^ab	1.98±0.03^ab	12.29±1.58^ab	12.76±2.00^ab
F值		6.281	8.152	10.313	8.851	9.525	9.862
P值		0.005	0.002	0.001	0.000	0.000	0.000

分组	例数	IgG	IgA	IgM	C3	C4
对照组	120	14.12±1.33	2.81±0.19	2.11±0.05	1.64±0.01	0.29±0.01
脑梗死轻症组	60	12.11±1.52^a	3.12±0.42^a	2.25±0.02^a	1.91±0.02^a	0.34±0.02^a
脑梗死中重症组	60	10.60±1.06^ab	4.01±0.35^ab	2.60±0.05^ab	2.13±0.04^ab	0.39±0.01^ab
F值		7.258	13.102	5.213	8.808	7.253
P值		0.015	0.002	0.025	0.003	0.007

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