Difference of TM4SF1 expression between fibroblasts and UCMSC

doi:10.3877/cma.j.issn.2095-1221.2018.05.004

Abstract

Abstract:

Objective

To find the difference between fibroblasts and umbilical cord mesemchemal stem cells (UCMSC) in surface markers and differentiation.

Methods

Flow cytometric analysis of CD105, CD90, CD73, CD44, CD14, CD34, CD45, CD79a, HLA-DR, FSP-1 and TM4SF1 was performed for fibroblasts and UCMSC. Multilineage differentiation of fibroblasts and UCMSC into fat cells, cartilage cells and bone cells were induced. The expression of FSP-1 and TM4SF1 in fibroblasts and in UCMSC was assayed by RT-PCR. Data analysis between different groups was perfermed with independent-sample t test.

Results

Those markers which were found to be expressed on UCMSC were also expressed on fibroblasts in comparable levels (P > 0.05). Fibroblasts and UCMSC could differentiate into fat cells, cartilage cells and bone cells in vitro. Both fibroblasts and UCMSC were positive for FSP-1 (98.6±0.3323)﹪ vs (98.90±0.2665)﹪, (t?=?0.4677, P?=?0.5294). TM4SF1 was positive on UCMSC (97.23±0.2250)﹪, but negative on fibroblast (0.0082±0.0018)﹪(t?=?346.9, P?<?0.01).

Conclusion

There is a significant difference in expression of TM4SF1 between fibroblasts and UCMSC.

Key words: Umbilical cord mesemchemal stem cells, Fibroblast, TM4SF1

Juan Yang, Xun Chai, Gentao Liu. Difference of TM4SF1 expression between fibroblasts and UCMSC[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2018, 08(05): 278-283.

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Figures/Tables 7

References 34

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基因名称	正向引物序列	反向引物序列
FSP-1	AAAAGGACAGATGAAGCTGC	TCATTTCTTCCTGGGCTGCT
TM4SF1	CCTAGCAGACCACCA	GTCCTGGGTTGGTTCT
GAPDH	TCAGTGGTGGACCTGACCTG	TGCTGTAGCCAAATTGGTTG

基因名称	正向引物序列	反向引物序列
FSP-1	AAAAGGACAGATGAAGCTGC	TCATTTCTTCCTGGGCTGCT
TM4SF1	CCTAGCAGACCACCA	GTCCTGGGTTGGTTCT
GAPDH	TCAGTGGTGGACCTGACCTG	TGCTGTAGCCAAATTGGTTG

细胞分类	例数	CD14	CD34	CD45	CD79a	HLA-DR	CD44	CD73	CD90	CD105
成纤维细胞	5	0.12 ± 0.02	0.22 ± 0.10	0.18 ± 0.06	0.16 ± 0.02	0.14 ± 0.02	98.36 ± 0.27	98.02 ± 0.32	97.62 ± 0.51	97.96 ± 0.33
UCMSC	5	0.08 ± 0.02	0.12 ± 0.02	0.16 ± 0.04	0.18 ± 0.04	0.16 ± 0.02	97.82 ± 0.59	98.56 ± 0.28	98.16 ± 0.22	98.22 ± 0.39
t值	?	1.18	0.97	0.33	0.64	0.58	0.81	1.20	0.89	0.45
P值	?	0.20	0.34	0.78	0.67	0.58	0.43	0.24	0.36	0.62

细胞分类	例数	CD14	CD34	CD45	CD79a	HLA-DR	CD44	CD73	CD90	CD105
成纤维细胞	5	0.12 ± 0.02	0.22 ± 0.10	0.18 ± 0.06	0.16 ± 0.02	0.14 ± 0.02	98.36 ± 0.27	98.02 ± 0.32	97.62 ± 0.51	97.96 ± 0.33
UCMSC	5	0.08 ± 0.02	0.12 ± 0.02	0.16 ± 0.04	0.18 ± 0.04	0.16 ± 0.02	97.82 ± 0.59	98.56 ± 0.28	98.16 ± 0.22	98.22 ± 0.39
t值	?	1.18	0.97	0.33	0.64	0.58	0.81	1.20	0.89	0.45
P值	?	0.20	0.34	0.78	0.67	0.58	0.43	0.24	0.36	0.62

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