Analysis of factors associated with the quality of umbilical cord blood

doi:10.3877/cma.j.issn.2095-1221.2018.04.006

Abstract

Abstract:

Objective

To evaluate the maternal, infant and collection-processing factors associated with the quality of umbilical cord blood(UCB).

Methods

389 umbilical cord blood units were recorded, including collection volume, mother's age, gestational age, infant birth weight, delivery way, sex of newborn, number of pregnancy, time interval between collection and counting and collection method. The total nucleated cells (TNC) were counted by KX-21 automatic blood analyzer. Pearson correlation and Spearman rank correlation were used for correlation analysis, t test, mann-whitney U test, variance analysis and kruskal-wallis H test were used for group comparison, and the correlation factors affecting umbilical cord blood collection volume and TNC were analyzed.

Results

There was a significant correlation between collection volume and TNC (r = 0.723, P?< 0.001). As for maternal and infant factors, mother's age had no correlation between collection volume and TNC. Gestational age correlated negatively with collection volume (r = -0.119, P = 0.019) and positively with TNC (r = 0.138, P = 0.007). The TNC from full-term infants was significantly higher than that of preterm ones (P = 0.038). The infant birth weight correlated strongly with collection volume (r = 0.236, P < 0.001) and TNC (r = 0.275, P < 0.001). Although the collection volume of cesarean section was higher than that of vaginal delivery (P < 0.001), its TNC was lower than that of vaginal delivery. There was no difference between male and female infants in collection volume and TNC. As for collection-processing factors, the time interval between collection and counting had no correlation between collection volume and TNC.

Conclusion

These results suggest that infant weight and vaginal delivery are associated with TNC.

Key words: Umbilical cord blood, Ucleated cells, Influencing factors

Jin Zhang, Zhe Yuan. Analysis of factors associated with the quality of umbilical cord blood[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2018, 08(04): 224-228.

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Figures/Tables 2

References 10

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影响因素	采集量（ml）		TNC（×10⁸）
影响因素	r	P	r	P
年龄（岁）	0.105	0.058^*	0.002	0.967^*
孕龄（周）	-?0.119	0.019^*	0.138	0.007^*
新生儿体重（g）	0.236	< 0.001	0.275	< 0.001^*
采集至计数间隔（h）	0.149	0.103^*	0.081	0.109^*
采集量（ml）	?	?	0.723	< 0.001^*
TNC（×10⁸）	?	?	?	?

影响因素	采集量（ml）		TNC（×10⁸）
影响因素	r	P	r	P
年龄（岁）	0.105	0.058^*	0.002	0.967^*
孕龄（周）	-?0.119	0.019^*	0.138	0.007^*
新生儿体重（g）	0.236	< 0.001	0.275	< 0.001^*
采集至计数间隔（h）	0.149	0.103^*	0.081	0.109^*
采集量（ml）	?	?	0.723	< 0.001^*
TNC（×10⁸）	?	?	?	?

影响因素		例数（﹪）	采集量（ml）	TNC（×10⁸）
采集量（ml）		?	?	?
?	≤80	245（63）	?	5.5（4.1 ~ 6.9）
?	> 80	144（37）	?	9.1（7.3 ~ 11.8）
?	P值	?	?	< 0.001
孕龄（周）		?	?	?
?	36	33（9）	78±28	5.7（3.7 ~ 8.6）
?	37 ~ 41	356（91）	75±27	6.7（4.9 ~ 9.1）
?	P值	?	0.604	0.038
新生儿体重（g）		?	?	?
?	< 3 000	42（11）	60±21	4.2（3.6 ~ 6.1）
?	3 000 ~ 3 499	176（45）	74±25	6.4（4.8 ~ 9.0）
?	3 500 ~ 3 999	142（37）	79±29	7.3（5.5 ~ 9.1）
?	≥4 000	29（ 7）	88±32	8.9（6.4 ~ 12.3）
?	P值	?	< 0.001	< 0.001
分娩方式		?	?	?
?	阴道分娩	117（30）	64±23	6.7（4.9 ~ 9.1）
?	剖宫产	272（70）	80±28	6.6（4.7 ~ 9.0）
?	P值	?	< 0.001	0.971
新生儿性别		?	?	?
?	女性	185（48）	72（53 ~ 98）	6.9（5.2 ~ 9.3）
?	男性	204（52）	60（21 ~ 84）	6.4（4.5 ~ 8.9）
?	P值	?	0.435	0.061
胎次		?	?	?
?	1	196（50）	72±24	7.0±2.9
?	2	128（33）	77±30	7.1±3.2
?	3及以上	65（17）	81±29	7.9±4.3
?	P值	?	0.099	0.201
采集方式		?	?	?
?	第三产程	296（76）	76±28	6.7（4.9 ~ 9.1）
?	体外采集	46（12）	79±24	6.9（5.4 ~ 8.8）
?	三+体外	47（12）	65±27	5.2（3.9 ~ 9.3）
?	P值	?	0.022	0.037

影响因素		例数（﹪）	采集量（ml）	TNC（×10⁸）
采集量（ml）		?	?	?
?	≤80	245（63）	?	5.5（4.1 ~ 6.9）
?	> 80	144（37）	?	9.1（7.3 ~ 11.8）
?	P值	?	?	< 0.001
孕龄（周）		?	?	?
?	36	33（9）	78±28	5.7（3.7 ~ 8.6）
?	37 ~ 41	356（91）	75±27	6.7（4.9 ~ 9.1）
?	P值	?	0.604	0.038
新生儿体重（g）		?	?	?
?	< 3 000	42（11）	60±21	4.2（3.6 ~ 6.1）
?	3 000 ~ 3 499	176（45）	74±25	6.4（4.8 ~ 9.0）
?	3 500 ~ 3 999	142（37）	79±29	7.3（5.5 ~ 9.1）
?	≥4 000	29（ 7）	88±32	8.9（6.4 ~ 12.3）
?	P值	?	< 0.001	< 0.001
分娩方式		?	?	?
?	阴道分娩	117（30）	64±23	6.7（4.9 ~ 9.1）
?	剖宫产	272（70）	80±28	6.6（4.7 ~ 9.0）
?	P值	?	< 0.001	0.971
新生儿性别		?	?	?
?	女性	185（48）	72（53 ~ 98）	6.9（5.2 ~ 9.3）
?	男性	204（52）	60（21 ~ 84）	6.4（4.5 ~ 8.9）
?	P值	?	0.435	0.061
胎次		?	?	?
?	1	196（50）	72±24	7.0±2.9
?	2	128（33）	77±30	7.1±3.2
?	3及以上	65（17）	81±29	7.9±4.3
?	P值	?	0.099	0.201
采集方式		?	?	?
?	第三产程	296（76）	76±28	6.7（4.9 ~ 9.1）
?	体外采集	46（12）	79±24	6.9（5.4 ~ 8.8）
?	三+体外	47（12）	65±27	5.2（3.9 ~ 9.3）
?	P值	?	0.022	0.037

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