探究大鼠肝脏脱细胞的细胞外基质支架对胰岛细胞活性及分泌功能的影响，为1型糖尿病的胰岛移植治疗提供新型生物材料。

采用灌流脱细胞法处理大鼠肝脏，通过组织学染色、免疫荧光染色及扫描电子显微镜表征支架结构与核心成分；利用ELISA试剂盒检测支架中血小板源性生长因子、成纤维细胞生长因子等活性因子的保留情况；借助DNA含量检测、DAPI染色及L929细胞CCK-8实验评估支架生物相容性；以富血小板血浆（PRP）灌注对脱细胞支架进行功能化修饰；运用免疫荧光染色、细胞活力染色验证PRP修饰支架对胰岛细胞活性与功能的作用。两组间比较采用独立样本t检验；三组间比较采用单因素方差分析，组间两两比较采用LSD-t检验。双因素设计的采用两因素重复测量方差分析，并采用Bonferroni校正进行多重比较。

灌流法制备的肝脏脱细胞支架宏观呈半透明白色，保留天然多层级脉管结构，微观为多孔网状形态；多种染色显示脱细胞效果显著，且留存Ⅰ型胶原、纤维连接蛋白等核心基质蛋白及多种活性因子，其中成纤维细胞生长因子和糖胺聚糖相对保留率分别达（88.05 ± 9.87）%和（90.77 ± 3.32）%。该支架生物相容性良好，无细胞核且DNA残留量仅（5.25 ± 2.18）ng/mg,可促进L929细胞增殖。与对照组比较，支架组葡萄糖刺激胰岛素分泌实验的刺激指数（2.78 ± 0.08比2.33 ± 0.14）升高，差异具有统计学意义（P < 0.01）。PRP修饰后可提高支架中如血小板源性生长因子的生长因子含量，差异具有统计学意义（P < 0.05）；PRP修饰支架对胰岛细胞活性及功能发挥具有积极促进作用。

大鼠肝脏脱细胞支架制备工艺可靠，兼具结构完整性与生物活性，PRP修饰后的支架可有效支持胰岛细胞活力维持与功能发挥，为胰岛移植提供具有应用潜力的新型支架材料及技术思路。

To prepare the effects of rat liver decellularized extracellular matrix scaffold on pancreatic islet cell viability and secretory function, providing a novel biomaterial for pancreatic islet transplantation in type 1 diabetes treatment.

Rat livers were processed using perfusion decellularization. The scaffold structure and core components were characterized through histological staining, immunofluorescence staining, and scanning electron microscopy. The retention of bioactive factors such as platelet-derived growth factor and fibroblast growth factor in the scaffold was detected using ELISA kits. Biocompatibility was assessed via DNA content quantification, DAPI staining, and CCK-8 assay using L929 cells. Functional modification of the decellularized scaffold was achieved by perfusion with platelet-rich plasma (PRP). The effects of the PRP-modified scaffold on pancreatic islet cell viability and function were verified using immunofluorescence staining and cell viability staining. Comparisons between two groups were performed using independent samples t-test, comparisons among three groups were conducted using one-way analysis of variance (ANOVA), followed by LSD-t test for pairwise comparisons. For the two-factor design, a two-way repeated-measures ANOVA was performed, with Bonferroni correction applied for multiple comparisons.

The liver decellularized scaffold prepared by perfusion appeared translucent white macroscopically, retained the natural multi-level vascular structure, and exhibited a porous network morphology microscopically. Various staining methods confirmed significant decellularization efficiency with preservation of core matrix proteins such as type I collagen and fibronectin, as well as multiple bioactive factors. Specifically, the relative retention rates of fibroblast growth factor and glycosaminoglycans reached (88.05 ± 9.87)% and (90.77 ± 3.32)%, respectively. The scaffold demonstrated good biocompatibility, being acellular with residual DNA content as low as (5.25 ± 2.18) ng/mg, and significantly promoted L929 cell proliferation. The scaffold enhanced the insulin secretory function of pancreatic islet cells, the stimulation index in the glucose-stimulated insulin secretion assay for the scaffold group was significantly higher than that of the control group (2.78 ± 0.08 vs 2.33 ± 0.14), with statistical significance (P < 0.01). PRP modification significantly increased the content of growth factors, such as platelet-derived growth factor, in the scaffold, showing statistical significance (P < 0.05). The PRP-modified scaffold positively promoted pancreatic islet cell viability and function.

The preparation process for the rat liver decellularized scaffold is reliable, yielding a scaffold with both structural integrity and bioactivity. The PRP-modified scaffold effectively supports the maintenance of islet cell viability and function, presenting a promising novel scaffold material and technical strategy for islet transplantation.