切换至 "中华医学电子期刊资源库"

中华细胞与干细胞杂志(电子版) ›› 2025, Vol. 15 ›› Issue (01) : 57 -62. doi: 10.3877/cma.j.issn.2095-1221.2025.01.007

综述

间充质干细胞制备的纳米囊泡在疾病治疗中的研究进展
卓馨怡1, 祝宇翀2, 刘军权2, 廖雨琴2,()   
  1. 1. 310053 杭州,浙江卫未生物医药科技有限公司
    2. 310053 杭州,浙江中医药大学医学技术与信息工程学院2023级临床检验诊断学
  • 收稿日期:2024-07-15 出版日期:2025-02-01
  • 通信作者: 廖雨琴
  • 基金资助:
    国家自然科学基金 (82072512)

Advances in the application of mesenchymal stem cells-derived nanovesicle in various diseases

Xinyi Zhuo1, Yuchong Zhu2, Junquan Liu2, Yuqin Liao2,()   

  1. 1. Zhejiang Weiwei Biopharmaceutical Technology Co.,Ltd.,Hangzhou 310053,Zhejiang
    2. School of Medical Technology and Information Engineering,Zhejiang University of Traditional Chinese Medicine,Clinical Laboratory Diagnosis,Class of 2023,Hangzhou 310053,China
  • Received:2024-07-15 Published:2025-02-01
  • Corresponding author: Yuqin Liao
引用本文:

卓馨怡, 祝宇翀, 刘军权, 廖雨琴. 间充质干细胞制备的纳米囊泡在疾病治疗中的研究进展[J/OL]. 中华细胞与干细胞杂志(电子版), 2025, 15(01): 57-62.

Xinyi Zhuo, Yuchong Zhu, Junquan Liu, Yuqin Liao. Advances in the application of mesenchymal stem cells-derived nanovesicle in various diseases[J/OL]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2025, 15(01): 57-62.

间充质干细胞制备的纳米囊泡 (MSCNVs)作为一种新型的细胞治疗替代方案,具有稳定性高和体积小等优势,其应用前景已在神经系统疾病、心血管疾病、炎症性疾病和组织再生修复等多个领域得到证实。研究表明,纳米囊泡 (NVs)通过促进组织再生、减轻炎症反应和调节免疫应答等机制,在疾病的治疗和组织修复中发挥积极作用。然而,目前MSCNVs 在疾病应用中仍面临有效性和长期安全性等挑战,需进一步研究改进。本综述引用最新的研究成果和试验数据,系统性分析MSCNVs 在多种疾病治疗中的应用和疗效,以全面且更新的视角展望MSCNVs 在疾病治疗中的潜在价值。

Mesenchymal stem cell derived nanovesicles (MSCNVs) represent an innovative cellular therapy alternative, characterized by high stability and reduced size, with therapeutic potential validated across various fields, including the nervous system disease, cardiovascular health,inflammatory diseases, as well as tissue regeneration and repair. Studies indicate that nanovesicles(NVs) exert positive effects on disease treatment and tissue repair through mechanisms such as promoting tissue regeneration, alleviating inflammatory responses, and modulating immune responses. However, MSCNVs still confront challenges regarding efficacy and long-term safety in current disease applications, necessitating further research and optimization. This review integrates the latest research findings and experimental data, providing a systematic analysis of the applications and therapeutic efficacy of MSCNVs in the treatment of various diseases, offering a comprehensive and updated perspective on the potential value of MSCNVs in disease therapy.

表1 MSCNVs 在不同疾病领域中的文献研究成果汇总
1
Wang Y, Fang J, Liu B, et al. Reciprocal regulation of mesenchymal stem cells and immune responses[J]. Cell Stem Cell, 2022, 29(11):1515-1530.
2
Liu J, Gao J, Liang Z, et al. Mesenchymal stem cells and their microenvironment[J]. Stem Cell Res Ther, 2022, 13(1):429. doi:10.1186/s13287-022-02985-y.
3
朱淑芳,牟丽莎. 间充质干细胞在1 型糖尿病胰岛移植中的应用进展[J]. 器官移植, 2024, 15 (2): 214-219.
4
Yao Y, Huang J, Geng Y, et al. Paracrine action of mesenchymal stem cells revealed by single cell gene profiling in infarcted murine hearts[J].PLoS One, 2015, 10(6):e0129164. doi:10.1371/journal.pone.0129164.
5
Jo W, Kim J, Yoon J, et al. Large-scale generation of cell-derived nanovesicles[J]. Nanoscale, 2014, 6(20):12056-12064.
6
Liao Z, Liu C, Wang L, et al. Therapeutic role of mesenchymal stem cell-derived extracellular vesicles in female reproductive diseases[J].Front Endocrinol (Lausanne), 2021, 12:665645. doi:10.3389/fendo.2021.665645.
7
Sun D, Mou S, Chen L, et al. High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration[J]. Biomater Res, 2022, 26(1):83. doi:10.1186/s40824-022-00325-y.
8
Kim HY, Kim TJ, Kang L, et al. Mesenchymal stem cell-derived magnetic extracellular nanovesicles for targeting and treatment of ischemic stroke[J]. Biomaterials, 2020, 243:119942. doi:10.1016/j.biomaterials.2020.119942.
9
Lee JR, Park BW, Kim J, et al. Nanovesicles derived from iron oxide nanoparticles-incorporated mesenchymal stem cells for cardiac repair[J]. Sci Adv, 2020, 6(18):eaaz0952. doi: 10.1126/sciadv.aaz0952.
10
Park KS, Svennerholm K, Shelke GV, et al. Mesenchymal stromal cell- derived nanovesicles ameliorate bacterial outer membrane vesicle- induced sepsis via IL-10[J]. Stem Cell Res Ther, 2019,10(1):231. doi: 10.1186/s13287-019-1352-4.
11
Han C, Jeong D, Kim B, et al. Mesenchymal stem cell engineered nanovesicles for accelerated skin wound closure[J]. ACS Biomater Sci Eng, 2019, 5(3):1534-1543.
12
Zheng G, Huang R, Qiu G, et al. Mesenchymal stromal cell-derived extracellular vesicles: regenerative and immunomodulatory effects and potential applications in sepsis[J]. Cell Tissue Res, 2018, 374(1):1-15.
13
Widera D. Recent advances in translational adipose-derived stem cell biology[J]. Biomolecules, 2021, 11(11):1660. doi: 10.3390/biom11111660.
14
Mishra VK, Shih HH, Parveen F, et al. Identifying the therapeutic significance of mesenchymal stem cells[J]. Cells, 2020, 9(5):1145. doi:10.3390/cells9051145.
15
Khayambashi P, Iyer J, Pillai S, et al. Hydrogel encapsulation of mesenchymal stem cells and their derived exosomes for tissue engineering[J]. Int J Mol Sci, 2021, 22(2):684. doi: 10.3390/ijms22020684.
16
Lan T, Luo M, Wei X. Mesenchymal stem/stromal cells in cancer therapy[J]. J Hematol Oncol, 2021, 14(1):195. doi:10.1186/s13045-021-01208-w.
17
Timaner M, Tsai KK, Shaked Y. The multifaceted role of mesenchymal stem cells in cancer[J]. Semin Cancer Biol, 2020, 60:225-237.
18
Daltro SRT, Meira CS, Santos IP, et al. Mesenchymal stem cells and atopic dermatitis: a review[J]. Front Cell Dev Biol, 2020, 8:326. doi:10.3389/fcell.2020.00326.
19
Samadi P, Saki S, Manoochehri H, et al. Therapeutic applications of mesenchymal stem cells: a comprehensive review[J]. Curr Stem Cell Res Ther, 2021, 16(3):323-353.
20
Xu Q, Hou W, Zhao B, et al. Mesenchymal stem cells lineage and their role in disease development[J]. Mol Med, 2024, 30(1):207. doi:10.1186/s10020-024-00967-9.
21
Yu H, Huang Y, Yang L. Research progress in the use of mesenchymal stem cells and their derived exosomes in the treatment of osteoarthritis[J]. Ageing Res Rev, 2022, 80:101684. doi: 10.1016/j.arr.2022.101684.
22
Wadhwa S, Garg V, Gulati M, et al. Nanovesicles for nanomedicine:theory and practices[J]. Methods Mol Biol, 2019, 2000:1-17.
23
Yáñez-Mó M, Siljander PR, Andreu Z, et al. Biological properties of extracellular vesicles and their physiological functions[J]. J Extracell Vesicles, 2015, 4:27066. doi: 10.3402/jev.v4.27066. eCollection 2015.
24
Zhao Q, Hai B, Kelly J, et al. Extracellular vesicle mimics made from iPS cell-derived mesenchymal stem cells improve the treatment of metastatic prostate cancer[J]. Stem Cell Res Ther, 2021, 12(1):29. doi:10.1186/s13287-020-02097-5.
25
Jang SC, Kim OY, Yoon CM, et al. Bioinspired exosome-mimetic nanovesicles for targeted delivery of chemotherapeutics to malignant tumors[J]. ACS Nano, 2013, 7(9):7698-7710.
26
Salunkhe S, Dheeraj, Basak M, et al. Surface functionalization of exosomes for target-specific delivery and in vivo imaging & tracking:Strategies and significance[J]. J Control Release, 2020, 326:599-614.
27
Bai C, Liu J, Zhang X, et al. Research status and challenges of plant- derived exosome-like nanoparticles[J]. Biomed Pharmacother,2024, 174:116543. doi: 10.1016/j.biopha.2024.116543.
28
Guo Y, Wang H, Huang L, et al. Small extracellular vesicles-based cell- free strategies for therapy[J]. MedComm (2020), 2021, 2(1):17-26.
29
Lian MQ, Chng WH, Liang J, et al. Plant-derived extracellular vesicles: Recent advancements and current challenges on their use for biomedical applications[J]. J Extracell Vesicles, 2022, 11(12):e12283.doi: 10.1002/jev2.12283.
30
Shen Q, Huang Z, Yao J, et al. Extracellular vesicles-mediated interaction within intestinal microenvironment in inflammatory bowel disease[J]. J Adv Res, 2022, 37:221-233.
31
Lei Q, Gao F, Liu T, et al. Extracellular vesicles deposit PCNA to rejuvenate aged bone marrow-derived mesenchymal stem cells and slow age-related degeneration[J]. Sci Transl Med, 2021, 13(578):eaaz8697. doi: 10.1126/scitranslmed.aaz8697.
32
Matsuzaka Y, Yashiro R. Therapeutic strategy of mesenchymal-stemcell-derived extracellular vesicles as regenerative medicine[J]. Int J Mol Sci, 2022, 23(12):6480. doi: 10.3390/ijms23126480.
33
Dad HA, Gu TW, Zhu AQ, et al. Plant exosome-like nanovesicles:emerging therapeutics and drug deliverynanoplatforms[J]. Mol Ther,2021, 29(1):13-31.
34
Huang P, Wang L, Li Q, et al. Atorvastatin enhances the therapeutic efficacy of mesenchymal stem cells-derived exosomes in acute myocardial infarction via up-regulating long non-coding RNA H19[J].Cardiovasc Res, 2020, 116(2):353-367.
35
Huang R, Jia B, Su D, et al. Plant exosomes fused with engineered mesenchymal stem cell-derived nanovesicles for synergistic therapy of autoimmune skin disorders[J]. J Extracell Vesicles, 2023,12(10):e12361. doi: 10.1002/jev2.12361.
36
Lai RC, Yeo RW, Tan KH, et al. Mesenchymal stem cell exosome ameliorates reperfusion injury through proteomic complementation[J].Regen Med, 2013, 8(2):197-209.
37
Bucan V, Vaslaitis D, Peck CT, et al. Effect of exosomes from rat adipose-derived mesenchymal stem cells on neurite outgrowth and sciatic nerve regeneration after crush injury[J]. Mol Neurobiol, 2019,56(3):1812-1824.
38
Jiang Y, Yu M, Song ZF, et al. Targeted delivery of mesenchymal stem cell-derived bioinspired exosome-mimetic nanovesicles with platelet membrane fusion for atherosclerotic treatment[J]. Int J Nanomedicine,2024, 19:2553-2571.
39
YY Ma, X Zhao, JY Chen, et al. Umbilical cord mesenchymal-stemcell-derived nanovesicles as a novel strategy to promote wound healing in diabetes[J]. Nano Select, 2023, 4(2):170-180.
40
Neupane YR, Handral HK, Alkaff SA, et al. Cell-derived nanovesicles from mesenchymal stem cells as extracellular vesicle-mimetics in wound healing[J]. Acta Pharm Sin B, 2023, 13(5):1887-1902.
41
Kim HY, Bhang SH. Stem cell-engineered nanovesicles exert proangiogenic and neuroprotective effects[J]. Materials (Basel), 2021,14(5):1078. doi: 10.3390/ma14051078.
42
Han C, Yang J, Sun J, et al. Extracellular vesicles in cardiovascular disease: biological functions and therapeutic implications[J]. Pharmacol Ther, 2022, 233:108025. doi: 10.1016/j.pharmthera.2021.108025.
43
Lu X, Xu Z, Shu F, et al. Reactive oxygen species responsive multifunctional fusion extracellular nanovesicles: prospective treatments for acute heart transplant rejection[J]. Adv Mater, 2024,36(35):e2406758. doi: 10.1002/adma.202406758.
44
Zhu Y, Liao ZF, Mo MH, et al. Mesenchymal stromal cell-derived extracellular vesicles for vasculopathies and angiogenesis: therapeutic applications and optimization[J]. Biomolecules, 2023, 13(7):1109. doi:10.3390/biom13071109.
45
Cheng J, Huang H, Chen Y, et al. Nanomedicine for diagnosis and treatment of atherosclerosis[J]. Adv Sci (Weinh), 2023,10(36):e2304294. doi: 10.1002/advs.202304294.
46
Ko KW, Yoo YI, Kim JY, et al. Attenuation of tumor necrosis factor- alpha induced inflammation by umbilical cord-mesenchymal stem cell derived exosome-mimetic nanovesicles in endothelial cells[J].Tissue Eng Regen Med, 2020, 17(2):155-163.
47
Ma J, Chen L, Zhu X, et al. Mesenchymal stem cell-derived exosomal miR-21a-5p promotes M2 macrophage polarization and reduces macrophage infiltration to attenuate atherosclerosis[J]. Acta Biochim Biophys Sin (Shanghai), 2021, 53(9):1227-1236.
48
Song Y, Dou H, Li X, et al. Exosomal miR-146a contributes to the enhanced therapeutic efficacy of interleukin-1beta-primed mesenchymal stem cells against sepsis[J]. Stem Cells, 2017, 35(5):1208-1221.
49
Wang X, Gu H, Qin D, et al. Exosomal miR-223 contributes to mesenchymal stem cell-elicited cardioprotection in polymicrobial sepsis[J]. Sci Rep, 2015, 5:13721. doi: 10.1038/srep13721.
50
Lotfy A, AboQuella NM, Wang H. Mesenchymal stromal/stem cell(MSC)-derived exosomes in clinical trials[J]. Stem Cell Res Ther,2023, 14(1):66. doi: 10.1186/s13287-023-03287-7.
51
You J, Fu Z, Zou L. Mechanism and potential of extracellular vesicles derived from mesenchymal stem cells for the treatment of infectious diseases[J]. Front Microbiol, 2021, 12:761338. doi: 10.3389/fmicb.2021.761338.
52
Xi Y, Ju R, Wang Y. Mesenchymal stem cell-derived extracellular vesicles for the treatment of bronchopulmonary dysplasia[J]. Front Pediatr, 2022, 10:852034. doi: 10.3389/fped.2022.852034.
53
Ma W, Zhang X, Liu Y, et al. Polydopamine decorated microneedles with Fe-MSC-derived nanovesicles encapsulation for wound healing[J]. Adv Sci (Weinh), 2022, 9(13):e2103317. doi: 10.1002/advs.202103317.
54
Wang L, Qiao S, Xia R, et al. Mesenchymal stromal cell-derived magnetic nanovesicles for enhanced skin retention and hair follicle growth[J]. Cytotherapy, 2023, 25(11):1176-1185.
55
Pang L, Jin H, Lu Z, et al. Treatment with mesenchymal stem cell- derived nanovesicle-containing gelatin methacryloyl hydrogels alleviates osteoarthritis by modulating chondrogenesis and macrophage polarization[J]. Adv Healthc Mater, 2023, 12(17):e2300315. doi:10.1002/adhm.202300315.
56
Jo W, Jeong D, Kim J, et al. Self-renewal of bone marrow stem cells by nanovesicles engineered from embryonic stem cells[J]. Adv Healthc Mater, 2016, 5(24):3148-3156.
57
Pandey S, Dvorakova MC. Future perspective of diabetic animal models[J]. Endocr Metab Immune Disord Drug Targets, 2020, 20(1):25-38.
58
Pathi SD, Acevedo JF, Keller PW, et al. Recovery of the injured external anal sphincter after injection of local or intravenous mesenchymal stem cells[J]. Obstet Gynecol, 2012, 119(1):134-144.
59
Zhao M, Liu S, Wang C, et al. Mesenchymal stem cell-derived extracellular vesicles attenuate mitochondrial damage and inflammation by stabilizing mitochondrial DNA[J]. ACS Nano, 2021, 15(1):1519-1538.
60
Bandeira E, Jang SC, Lässer C, et al. Effects of mesenchymal stem cell- derived nanovesicles in experimental allergic airway inflammation[J]. Respir Res, 2023, 24(1):3.
61
Shi MM, Yang QY, Monsel A, et al. Preclinical efficacy and clinical safety of clinical-grade nebulized allogenic adipose mesenchymal stromal cells-derived extracellular vesicles[J]. J Extracell Vesicles,2021, 10(10):e12134.
62
Zhao Q, Hai B, Zhang X, et al. Biomimetic nanovesicles made from iPS cell-derived mesenchymal stem cells for targeted therapy of triple- negative breast cancer[J]. Nanomedicine, 2020, 24:102146.
63
Farinazzo A, Angiari S, Turano E, et al. Nanovesicles from adipose- derived mesenchymal stem cells inhibit T lymphocyte trafficking and ameliorate chronic experimental autoimmune encephalomyelitis[J]. Sci Rep, 2018, 8(1):7473.
64
Chu M, Wang H, Bian L, et al. Nebulization therapy with umbilical cord mesenchymal stem cell-derived exosomes for COVID-19 pneumonia[J]. Stem Cell Rev Rep, 2022, 18(6):2152-2163.
65
Hermann P, Appleby B, Brandel JP, et al. Biomarkers and diagnostic guidelines for sporadic Creutzfeldt-Jakob disease[J]. Lancet Neurol,2021, 20(3):235-246.
[1] 王彦, 张晓航, 冉素真, 钟春燕, 张晋炜, 王希. 双胎贫血-红细胞增多序列征的产前超声特征分析[J/OL]. 中华医学超声杂志(电子版), 2025, 22(05): 462-469.
[2] 朱郁芳, 宋冀军, 徐薇薇, 谢晶, 元艳, 秦晓霞. “互联网+入户护理”伤口评估体系的构建及应用效果[J/OL]. 中华损伤与修复杂志(电子版), 2025, 20(04): 325-332.
[3] 华义, 王华, 杨旭. 习惯性髌骨脱位的治疗现状与进展[J/OL]. 中华损伤与修复杂志(电子版), 2025, 20(04): 347-351.
[4] 毕小成, 王文霞, 邵金龙, 葛少华. Ⅳ期牙周炎伴病理性牙移位患者的多学科联合治疗[J/OL]. 中华口腔医学研究杂志(电子版), 2025, 19(04): 229-239.
[5] 杨志, 夏雪峰, 管文贤. DeepSurv深度学习模型辅助胃癌术后精准化疗策略研究[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(05): 501-505.
[6] 吴少锋, 王茂, 马海龙, 史英, 代引海. 新辅助治疗后肿瘤退缩分级对局部进展期直肠癌患者全直肠系膜切除术效果的临床研究[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(05): 535-538.
[7] 徐其银, 韩尚志. 术前结合术后营养支持对直肠癌患者康复的影响[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(05): 543-546.
[8] 吴菲, 袁媛, 何凡, 杜秋丽, 窦婷, 阮剑. 超声定位下冷循环射频消融术治疗甲状腺良性结节的疗效及预后分析[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(05): 562-565.
[9] 高峰, 郝少龙, 孙浩, 韩威. 三级淋巴结构在胰腺癌中的研究进展[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(05): 570-573.
[10] 曾华东, 邹雄峰, 李舒凡. 荧光腹腔镜下成功治疗自发性肝外胆汁瘤一例[J/OL]. 中华肝脏外科手术学电子杂志, 2025, 14(04): 628-630.
[11] 苟亚妮, 申群斌, 张丽, 卢佳佳. 幽门螺杆菌根除治疗与肠道菌群失调的互作机制、挑战与干预策略探究[J/OL]. 中华结直肠疾病电子杂志, 2025, 14(04): 359-363.
[12] 王乾宇, 郭寒川, 吴斌. 瘤内菌群在结直肠癌中的作用研究进展[J/OL]. 中华结直肠疾病电子杂志, 2025, 14(04): 370-374.
[13] 中国医师协会结直肠肿瘤专业委员会免疫学组. 结直肠癌免疫治疗专家共识(2025版)[J/OL]. 中华结直肠疾病电子杂志, 2025, 14(04): 289-298.
[14] 马燕红. 基于结构与功能评估的骨科康复CEST临床思路体系[J/OL]. 中华老年骨科与康复电子杂志, 2025, 11(04): 245-250.
[15] 慕佳霖, 孙萌, 李育霖, 邹卉. 甲基丙二酸血症合并肾脏并发症的发生机制和治疗研究进展[J/OL]. 中华临床医师杂志(电子版), 2025, 19(05): 382-387.
阅读次数
全文


摘要


AI


AI小编
你好!我是《中华医学电子期刊资源库》AI小编,有什么可以帮您的吗?