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中华细胞与干细胞杂志(电子版) ›› 2023, Vol. 13 ›› Issue (05) : 310 -314. doi: 10.3877/cma.j.issn.2095-1221.2023.05.008

综述

组织工程策略延缓椎间盘退变的现状与前景
韩明, 赵岩()   
  1. 010030 呼和浩特市,内蒙古自治区呼和浩特市内蒙古医科大学骨科学
    010030 呼和浩特市,内蒙古医科大学第二附属医院脊柱外科
  • 收稿日期:2023-11-15 出版日期:2023-10-01
  • 通信作者: 赵岩
  • 基金资助:
    内蒙古自治区自然科学基金(2022MS08016)

Status and prospects of tissue engineering strategies to retard intervertebral disc degeneration

Ming Han, Yan Zhao()   

  1. Inner Mongolia Medical University, Hohhot 010030, China
    Department of Spine Surgery, the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, China
  • Received:2023-11-15 Published:2023-10-01
  • Corresponding author: Yan Zhao
引用本文:

韩明, 赵岩. 组织工程策略延缓椎间盘退变的现状与前景[J]. 中华细胞与干细胞杂志(电子版), 2023, 13(05): 310-314.

Ming Han, Yan Zhao. Status and prospects of tissue engineering strategies to retard intervertebral disc degeneration[J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2023, 13(05): 310-314.

引起慢性腰痛的原因之一是椎间盘退变,目前的治疗方法均存在一定的缺陷。近年来研究发现组织工程策略延缓椎间盘退变的方式可能解决根源问题,其方式主要集中于多种生物材料与细胞、药物和活性因子等有机结合植入退变的椎间盘来实现。现有大量实验证明其可行性及优越性,但此研究仍处于基础研究阶段。本文回顾总结了不同组织工程方式延缓椎间盘退变的研究进展并分析其问题及前景。

One of the causes of chronic low back pain is intervertebral disc degeneration, and current treatments all have different shortcomings. In recent years, researchers have found that tissue engineering strategies to slow down disc degeneration may address the root cause of the problem, which mainly focuses on the implantation of a variety of biomaterials into degenerated discs with the organic combination of cells, drugs, active factors, etc. The feasibility and superiority of this approach have been demonstrated in many experiments. Many of existing experiments have proved its feasibility and superiority, but this research is still in the basic research stage. This paper reviews and summarizes the research progress of different tissue engineering methods to delay disc degeneration and analyzes their problems and prospects.

表1 不同组织工程策略特点及作用
第一作者 发表年份 材料组成 搭载物质 作用
Wang [19] 2023 二氧化硅和空心多巴胺 氨硼烷 抑制细胞外基质降解和减轻氧化应激和炎症
Li [24] 2021 聚己内酯和羟基磷灰石支架 骨髓间充质干细胞 加速椎间融合,并增强骨髓间充质干细胞的旁分泌功能
Wang [25] 2021 自组装明胶纳米颗粒凝胶 间充质干细胞 细胞量、糖胺聚糖含量、椎间盘高度指数和MRI指数均有显著改善
赵宇朋[26] 2021 甲基丙烯酸明胶水凝胶 髓核间充质干细胞及纤维环源性干细胞 COL-I、COL-II、GAG蛋白表达量均增高
叶旭文[27] 2023 甲基丙烯酸酰化明胶 姜黄素 抑制H2O2诱导的髓核细胞凋亡,降低氧化应激水平
Xu [28] 2020 明胶甲基丙烯酰微球 生长分化因子-5 增强了干细胞向髓核样表型的分化
Xing [33] 2021 细胞外基质水凝胶 脂肪间充质干细胞外泌体 调节基质合成和降解,并通过减轻炎症反应来抑制焦亡
BorrelliC[34] 2020 自组装细胞外基质水凝胶 硫酸软骨素 糖胺聚糖产生增加,并增强其合成髓核样基质的能力
Chen[35] 2022 聚乙醇酸-聚乙二醇水凝胶 贝伐珠单抗 MMP3表达降低,COL II合成促进
Sun[36] 2022 卵磷脂胶束 辅酶Q10 提高了大鼠骨髓间充质干细胞的活力,恢复了线粒体结构和功能,并增强了细胞外基质组分的合成
Feng[37] 2020 聚乳酸-共乙醇酸 骨髓间充质基质细胞与抗miR-199a 促进髓核表型,产生功能性细胞外基质
Wang[38] 2021 N-异丙基丙烯酰胺基热敏水凝胶 SHP099 胶原蛋白Ⅱ和糖胺聚糖的表达增高
1
Wang L, Ye H, Li Z, et al. Epidemiological trends of low back pain at the global, regional, and national levels[J]. Eur Spine J, 2022, 31(4):953-962.
2
Zheng J, Shen C. Quantitative relationship between the degree of lumbar disc degeneration and intervertebral disc height in patients with low back pain[J]. Contrast Media Mol Imaging, 2022, 2022:5960317. doi: 10.1155/2022/5960317.
3
Wei FL, Zhou CP, Zhu KL, et al. Comparison of different operative approaches for lumbar disc herniation: a network meta-analysis and systematic review[J]. Pain Physician, 2021, 24(4):E381-E392.
4
Kirnaz S, Capadona C, Wong T, et al. Fundamentals of intervertebral disc degeneration[J]. World Neurosurg, 2022, 157:264-273.
5
Li C, Bai Q, Lai Y, et al. Advances and prospects in biomaterials for intervertebral disk regeneration[J]. Front Bioeng Biotechnol, 2021, 9:766087. doi: 10.3389/fbioe.2021.766087.
6
杨林, 石军, 郭中华, 等. 基于高分子材料的椎间盘组织工程:研究重点和热点[J]. 中国组织工程研究, 2021, 25(16):2589-2596.
7
梁辰, 朱同贺, 朱忆尧, 等. 生物医用支架仿生设计及在组织工程中的应用[J]. 中国组织工程研究, 2024, 28(15):2416-2422.
8
Yan C, Wang X, Xiang C, et al. Applications of functionalized hydrogels in the regeneration of the intervertebral disc[J]. Biomed Res Int, 2021, 2021:2818624. doi: 10.1155/2021/2818624.
9
祝昊, 尚晖, 张洋, 等. 高分子材料在髓核组织工程中的应用与研究[J]. 中国医学工程, 2022, 30(05):53-58.
10
Ho CY, Wang CC, Wu TC, et al. Peptide-functionalized double network hydrogel with compressible shape memory effect for intervertebral disc regeneration[J]. Bioeng Transl Med, 2022, 8(2):e10447. doi: 10.1002/btm2.10447.
11
Wagner EK, Vedadghavami A, Jacobsen TD, et al. Avidin grafted dextran nanostructure enables a month-long intra-discal retention[J]. Sci Rep, 2020, 10(1):12017. doi: 10.1038/s41598-020-68351-1.
12
Lv B, Lu L, Hu L, et al. Recent advances in GelMA hydrogel transplantation for musculoskeletal disorders and related disease treatment[J]. Theranostics, 2023, 13(6):2015-2039.
13
马小艺, 陈海斌. 纳米材料在生物医学领域的应用与前景展望[J]. 中国医药导报, 2006, 3(32):13-15.
14
丁丽娜. 聚乳酸及其共聚物微球的制备及在生物医学领域的应用研究[D]. 东北师范大学, 2021.
15
Khandaker M, Kotturi H, Progri H, et al. In vitro and in vivo effect of polycaprolactone nanofiber coating on polyethylene glycol diacrylate scaffolds for intervertebral disc repair[J]. Biomed Mater, 2021, 16(4). doi: 10.1088/1748-605X/abfd12.
16
Liu Z, Wang H, Yuan Z, et al. High-resolution 3D printing of angle-ply annulus fibrosus scaffolds for intervertebral disc regeneration[J]. Biofabrication, 2022, 15(1). doi: 10.1088/1758-5090/aca71f.
17
宋婵婵, 冉兵, 宗毅, 等. 椎间盘退变机制及修复生物工程支架研究进展[J]. 中国疼痛医学杂志, 2022, 28(09):657-663.
18
Li Z, Cai F, Tang J, et al. Oxygen metabolism-balanced engineered hydrogel microspheres promote the regeneration of the nucleus pulposus by inhibiting acid-sensitive complexes[J]. Bioact Mater, 2022, 24:346-360.
19
Wang W, Xiao B, Qiu Y, et al. pH-responsive delivery of H2 through ammonia borane-loaded hollow polydopamine for intervertebral disc degeneration therapy[J]. Oxid Med Cell Longev, 2023, 2023:7773609. doi: 10.1155/2023/7773609.
20
王向阳, 刘永辉, 石洋, 等. 间充质干细胞在椎间盘退变中作用[J]. 中国矫形外科杂志, 2022, 30(10):906-910.
21
吴德升. 椎间盘退变干细胞生物学修复的临床研究进展与挑战[J]. 中国临床新医学, 2023, 16(3):201-206.
22
邓静倩, 侯丹丹, 祁丽亚, 等. 高分子微球在三维细胞培养领域的应用进展[J]. 化工新型料, 2023, 51(S2):155-160
23
卢世浩, 张子凡, 孙柏峰, 等. 间充质干细胞移植治疗椎间盘退变植入途径的研究进展[J]. 中国脊柱脊髓杂志, 2023, 33(11):1052-1056.
24
Li W, Huang C, Ma T, et al. Low-frequency electromagnetic fields combined with tissue engineering techniques accelerate intervertebral fusion[J]. Stem Cell Res Ther, 2021, 12(1):143. doi: 10.1186/s13287-021-02207-x.
25
Wang Y, Zhang Y, Chen K, et al. Injectable nanostructured colloidal gels resembling native nucleus pulposus as carriers of mesenchymal stem cells for the repair of degenerated intervertebral discs[J]. Mater Sci Eng C Mater Biol Appl, 2021, 128:112343. doi: 10.1016/j.msec.2021.112343.
26
赵宇朋. 负载髓核间充质干细胞及纤维环源性干细胞3D环状梯度GelMA水凝胶多模态一体化支架对椎间盘退变的实验研究[D]. 蚌埠医学院, 2021.
27
叶旭文, 顾勇, 陈亮. 负载姜黄素可注射微球延缓椎间盘的退变[J]. 中国组织工程研究, 2023, 27(12):1884-1891.
28
Xu H, Sun M, Wang C, et al. Growth differentiation factor-5-gelatin methacryloyl injectable microspheres laden with adipose-derived stem cells for repair of disc degeneration[J]. Biofabrication, 2020, 13(1):015010. doi: 10.1088/1758-5090/abc4d3.
29
李谦. 富血小板血浆治疗腰椎间盘突出症的研究进展[J]. 微创医学, 2023, 18(2):238-240.
30
杜薇, 丁宇, 崔洪鹏, 等. 经皮内窥镜下椎间盘摘除联合富血小板血浆凝胶微球置入治疗腰椎间盘突出症的疗效观察[J]. 中国脊柱脊髓杂志, 2020, 30(11):1001-1006.
31
赵文杰, 张文捷, 张亮, 等. 间充质干细胞来源外泌体在椎间盘退变修复中的研究进展[J/OL]. 中华损伤与修复杂志(电子版), 2021, 16(2):153-157.
32
杨超强, 张虎林, 汪小敏, 等. 间充质干细胞来源外泌体治疗椎间盘退变的机制研究进展[J]. 风湿病与关节炎, 2023, 12(5):66-70.
33
Xing H, Zhang Z, Mao Q, et al. Injectable exosome-functionalized extracellular matrix hydrogel for metabolism balance and pyroptosis regulation in intervertebral disc degeneration[J]. J Nanobiotechnology, 2021, 19(1):264. doi: 10.1186/s12951-021-00991-5.
34
Borrelli C, Buckley CT. Injectable Disc-Derived ECM Hydrogel Functionalised with Chondroitin Sulfate for Intervertebral Disc Regeneration[J]. Acta Biomater, 2020, 117:142-155.
35
Chen Q, Wang J, Xia Q, et al. Treatment outcomes of injectable thermosensitive hydrogel containing bevacizumab in intervertebral disc degeneration[J]. Front Bioeng Biotechnol, 2022, 10:976706. doi: 10.3389/fbioe.2022.976706.
36
Sun J, Yang F, Wang L, et al. Delivery of coenzyme Q10 loaded micelle targets mitochondrial ROS and enhances efficiency of mesenchymal stem cell therapy in intervertebral disc degeneration[J]. Bioact Mater, 2022, 23:247-260.
37
Feng G, Zhang Z, Dang M, et al. Nanofibrous spongy microspheres to deliver rabbit mesenchymal stem cells and anti-miR-199a to regenerate nucleus pulposus and prevent calcification[J]. Biomaterials, 2020, 256:120213. doi: 10.1016/j.biomaterials.2020.120213.
38
Wang J, Huang L, Huang Y, et al. Therapeutic effect of the injectable thermosensitive hydrogel loaded with SHP099 on intervertebral disc degeneration[J]. Life Sci, 2021, 266:118891. doi: 10.1016/j.lfs.2020.118891.
39
Wang Y, Kang J, Guo X, et al. Intervertebral disc degeneration models for pathophysiology and regenerative therapy-benefits and limitations[J]. J Invest Surg, 2022, 35(4):935-952.
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