探讨磷酸化应激诱导蛋白1 （STIP1）在乳腺癌组织中的表达情况及其与免疫细胞浸润之间的关系。

利用GEPIA2和Ualcan数据库进行STIP1在乳腺癌及不同分型中的表达差异分析；通过Kaplan-Meier Plotter分析STIP1表达与乳腺癌及不同分型患者生存的关系；使用TIMER数据库分析STIP1与不同分型乳腺癌免疫细胞浸润的相关性；并运用String数据库分析STIP1相互作用蛋白，采用Wilcoxon符号秩检验比较乳腺癌和邻近正常组织间STIP的表达差异，其他非匹配数据的比较，两组间比较采用Mann-Whitney U检验，多组间比较采用Kruskal-Wallis H检验，组间两两比较采用Dunn检验，Kaplan-Meier曲线用于分析STIP1表达差异在乳腺癌分型中的无复发生存时间（RFS）情况，Spearman相关系数用于评估免疫细胞浸润比例与STIP1表达水平间的相关性。

与正常组织比较，乳腺癌中STIP1表达上调，且在不同分型乳腺癌中均上调。STIP1高表达与乳腺癌患者的RFS降低相关，特别是在Luminal A型乳腺癌中。STIP1与CD8⁺ T细胞、CD4⁺ T细胞、中性粒细胞等多种免疫细胞浸润水平存在正相关性。STIP1与PTGES3、ASHA1、PRNP等多个蛋白存在相互作用，这些蛋白主要参与蛋白质折叠、蛋白质稳定、热应激反应、细胞周期和凋亡调控等生物学过程。

STIP1在乳腺癌中的表达上调，与患者的不良预后相关，特别是在Luminal A型乳腺癌中。STIP1可能在乳腺癌的免疫微环境中发挥重要作用，通过调节免疫细胞的功能状态影响肿瘤的发展和预后。

This study aims to investigate the expression of stress-induced phosphoprotein 1 (STIP1) in breast cancer tissues and its relationship with immune cell infiltration.

The GEPIA2 and Ualcan databases were used to analyze the expression difference of STIP1 in different types of breast cancer. Kaplan-Meier Plotter was used to analyze the relationship between the expression of STIP1 and the survival of breast cancer patients with different subtypes. TIMER database was used to analyze the correlation between STIP1 and immune cell infiltration in different types of breast cancer. The String database was used to analyze the STIP1 related interaction proteins. The difference expression of STIP1 between breast cancer and adjacent normal tissue is compared by Wilcoxon test. The difference of other indexes between two groups were compared by Mann-Whitney U test. The difference among groups were compared by Kruskal-Wallis H test, and pairwise comparisons between groups were conducted using the Dunn test. Kaplan-Meier curve was used to analyze the relapse-free survival (RFS) of STIP1 expression differences in different types of breast cancer, and Spearman correlation coefficient was used to evaluate the correlation between the proportion of immune cell infiltration and the expression level of STIP1.

Compared with normal tissues, STIP1 expression is upregulated in different types of breast cancer. High expression of STIP1 is associated with reduced RFS in breast cancer patients, especially in Luminal A breast cancer. STIP1 is positively correlated with the infiltration levels of various immune cells such as CD8⁺ T cells, CD4⁺ T cells, and neutrophils. STIP1 interacts with multiple proteins such as PTGES3, ASHA1, and PRNP, which are mainly involved in biological processes such as protein folding, protein stability, heat stress response, cell cycle, and apoptosis regulation.

The upregulation of STIP1 in breast cancer is associated with poor prognosis for patients, especially in Luminal A subtype breast cancer. STIP1 may play a significant role in the immune microenvironment of breast cancer, influencing tumor development and prognosis by modulating the functional status of immune cells.